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D-luciferin luminescent substrates
  10103.
 :  Biotium
 :  
D-luciferin luminescent substrates
  10103.
 :  Biotium
 :  

 

  • Pk:
    5 mg
  • Host:
  • Description:
    DMNPE-caged D-luciferin
  • Environmentally Preferable:

 

 

Luciferin is a substrate for the enzyme firefly luciferase.

D-luciferin potassium salt is the more commonly water soluble substrate for cell-based and in vivo reporter assays. D-luciferin sodium salt is an alternative salt form with higher water solubility. D-luciferin free acid is soluble in methanol or DMSO, but only sparingly soluble in water. DMNPE-caged D-luciferin is a D-luciferin ester derivative which can cross cell membranes efficiently. Once inside the cells, the ester is continuously hydrolysed to a supply of D-luciferin. Alternatively, a burst of D-luciferin release can be generated by UV photolysis.

Luciferase is encoded by the luc gene, which is widely used as a reporter gene. Luminescence at 560 nm is generated when luciferase catalyses the oxidation of luciferin by an ATP-dependent process. Cells and tissues have very low intrinsic bioiluminescence, allowing for highly sensitive detection of lucifease expression. In addition, luciferin/luciferase can be used for highly sensitive measurements of ATP levels.