illustra™ NICK™ columns
About this item
NICK™ columns were originally designed for purification of nick-translated DNA fragments but are also suitable for separation of any labelled probe from unincorporated labelled nucleotides.
- For rapid purification of labeled DNA (20 bases in length) from unincorporated radiolabeled nucleotides using gravity-flow chromatography
- Prepacked with Sephadex™ G-50 DNA Grade in distilled water with 0,15% Kathon CG/ICP Biocide
- Can accommodate sample volumes up to 100 μl
They are ready for immediate use, and operate by gravity flow. Tests show that at least 97% of radioactivity applied in a nick-translated mixture elutes in two well-separated peaks, corresponding to labeled DNA and unincorporated nucleotides, respectively. At least 90% of the applied DNA is recovered.
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Product Details & Documents
NICK™ columns were originally designed for purification of nick-translated DNA fragments but are also suitable for separation of any labelled probe from unincorporated labelled nucleotides.
- For rapid purification of labeled DNA (20 bases in length) from unincorporated radiolabeled nucleotides using gravity-flow chromatography
- Prepacked with Sephadex™ G-50 DNA Grade in distilled water with 0,15% Kathon CG/ICP Biocide
- Can accommodate sample volumes up to 100 μl
They are ready for immediate use, and operate by gravity flow. Tests show that at least 97% of radioactivity applied in a nick-translated mixture elutes in two well-separated peaks, corresponding to labeled DNA and unincorporated nucleotides, respectively. At least 90% of the applied DNA is recovered.
Documents
Specifications for Product Family
Specifications
Specifications
- Catalog No:
- 17-0855-01
- 17-0855-02
- Description:
- Illustra™ NICK™ columns
- Illustra™ NICK™ columns
- Target molecule:
- DNA
- DNA
- Refine target molecule:
- Amplified/modified DNA
- Amplified/modified DNA
- Sample type:
- PCR and other enzymatic reactions
- PCR and other enzymatic reactions
- Method / Format:
- Gel filtration
- Gel filtration
- Prep size:
- Scalable
- Scalable
Specifications
Product Family Options
Product Information
- PkAvailabilityPrice
- 17-0855-01Illustra™ NICK™ columnsSpecifications:
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Specifications
Cat. No.17-0855-01DescriptionIllustra™ NICK™ columnsTarget moleculeDNARefine target moleculeAmplified/modified DNASample typePCR and other enzymatic reactionsMethod / FormatGel filtrationPrep sizeScalable - 17-0855-02Illustra™ NICK™ columnsSpecifications:
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Specifications
Cat. No.17-0855-02DescriptionIllustra™ NICK™ columnsTarget moleculeDNARefine target moleculeAmplified/modified DNASample typePCR and other enzymatic reactionsMethod / FormatGel filtrationPrep sizeScalable
#: 20864.320
Supplier: VWR Chemicals
#: 83640.320
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck
#: 31770.294
Supplier: VWR Chemicals
#: 83645.320
Supplier: VWR Chemicals
#: 1.09439.1000
Supplier: Merck