You searched for: Microscopy and staining reagents

Supplier: Merck

Kaiser's glycerol gelatin is an aqueous mounting medium for microscopy and works well with specimens prepared for enzyme, lipid, and immunohistochemistry determinations.

Supplier: Merck

The defined hardness and uniformity of the material to be cut are vital requirements in creating consistent quality in cutting. These requirements can be met using a range of suitable substances, such as paraffins, resins, gelatine and celloidin.

Supplier: SIGMA ALDRICH MICROSCOPY

Zinc formalin fixative is a non-precipitating fixative. This fixative is compatible with histochemical, immunochemical and special stains. It may be used with automated tissue processors and also in manual methods. The zinc chloride component of the formulation increases the rigidity of cellular components to withstand subsequent processing, sectioning and staining procedures. Tissue specimens initially fixed using 10% neutral buffered formalin solution may be post-fixed using the zinc formalin fixative.

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion Oil has a defined refractive index of about 1,516. It is important that the refractive index (nD) is about 1,5, similar to that of glass. This enables a homogeneous oil immersion to be achieved.

Supplier: SIGMA ALDRICH MICROSCOPY

PolyFreeze is a water-soluble support matrix and a form of embedding medium for frozen sectioning. PolyFreeze sections flawlessly at routine frozen specimen thickness of 5-6 μ and leaves no slide residue. It provides for -20 ºC cryostat sectioning in rapid set embedment. This medium freezes quickly supporting the tissue for sectioning at 3 mm and up with no cracking of the matrix at temperatures from -8 °C to -25 °C.

Supplier: SIGMA ALDRICH MICROSCOPY

CC/mount is an aqueous based permanent mounting medium with a very high refractive index, which when applied to the stained tissue sections can store the tissue specimens permanently without the fading of the chromogens. Because of the superior refractive index of CC/mount, tissues mounted in this medium look like specimens mounted with organic based mounting media. No coverslipping is required with CC/mount. However, if coverslipping is required, dried CC/mount can be post mounted by using an organic based mounting medium. CC/mount permits the long-term storage of antigens localised using organic insoluble chromogen substrates, including AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT and fluorescent dyes like FITC and phycobiliproteins. The high pH of CC/mount ensures increased stability of fluorescence intensity.

Supplier: Merck

Entellan® new is a new mounting medium for cover slippers and is specially suited for commercial automated mounting instruments that operate with cover slippers. Its viscosity ranges between 250 and 600 mPas, 1,00869 has a small viscosity range. This narrow viscosity range improves and accelerates the application, as neither the dropping rate nor the volume need to be reset after the first run with a new bottle of Entellan® new.

Supplier: SIGMA ALDRICH MICROSCOPY

Suitable for use with large and small mineralized (undecalcified) bone sections. Yields clear, hard blocks for cutting sections. Not water-soluble.

Supplier: Merck

Hard tissue like bones or teeth, and all specimens of calcified tissue like bone marrow punches require special pre-treatment before routine processing by a histological laboratory.

Supplier: VWR Chemicals

Decalcifier according to Kristenson.

Supplier: VWR Chemicals

HistoChoice® clearing agent is a much less hazardous replacement for xylene/toluene.

Supplier: Avantor

Mounting medium for coverslipping of slides.

Supplier: VWR Chemicals

Fixing solution with 0,02% formaldehyde and 0,01% citric acid in aqueous solution.

Supplier: VWR Chemicals

Contains a mixture of hydrochloric acid and alcohols with some EDTA.

Supplier: Avantor

Mounting medium for coverslipping of slides.

Supplier: VWR Chemicals

Safemount Q Path® is a mounting medium, supplied in an PET bottle, which is compatible with Safesolv. It glues the coverglass to the slide and fixes the probe in position after staining.

Supplier: VWR Chemicals

High viscosity mounting medium for cryotomy.

Supplier: VWR Chemicals

Glue for improving adherance of histologicals sections to slides. Alternative to albumin-glycerol.

Supplier: Merck

Water-free mounting media, such as Neo-Mount®, should be used for permanent mounting and long-term storage of samples. Slides processed in pathologyl laboratories need to be stored up to 10 years, whereby the staining results need to be stable and fading-out is not acceptable. Neo-Mount®, anhydrous mounting medium for microscopy is an extremely colour-stable mounting medium for microscopy, which is produced with solvents based on mixtures of aliphatic hydrocarbons. It contains an aromatic-free substitute for xylene, thus, Neo-Mount® needs to be combined with Neo-Clear® exclusively. Xylene must be avoided in the mounting step, as it will cause the slides to become cloudy and streaked. In addition, by placing the dehydrated slides on filter paper for approx. 1 minute prior to mounting, any excess Neo-Clear® should be circumvented, as air bubbles might arise under the cover slip. The same pre-condition should also be met when mounting specimens using cover slip machines; in this area, Neo-Clear® can be most efficiently eliminated by incubating the slides for 1 minute in an empty slide rack.

Supplier: Avantor

Formalin (dissolved formaldehyde) is used in histopathology laboratories.

Supplier: VWR Chemicals

High viscosity mounting media for cryotomy. Less viscous than O.C.T.

Supplier: VWR Chemicals

Cytology fixative specially designed for the conservation and transport of cytology specimens.

Supplier: VWR Chemicals

A range of mounting media for manual and automatic mounting of slides. The Q Path® Coverquick range consists of four different mounting media with different viscosities to meet all microscopy needs. The number after the name indicates increasing viscosity: Q Path® Coverquick 1000 has the lower viscosity (around 260 mPaS) and Q Path® Coverquick 4000 has the higher viscosity (around 920 mPaS).

Supplier: VWR Chemicals

When sectioning frozen tissue for rapid surgical biopsy diagnosis or for research (typically in Histochemistry), the tissue sample must be firmly attached to the microtome stage or chuck. Embedding material such as OCT are preferred as these also provide a supportive and protective aid to sectioning.

Supplier: VWR Collection

Immersion oil is used to increase microscope resolution.

Supplier: VWR Chemicals

Fixative, combining the coagulant qualities of alcohol, to enable a better fixation of nuclear and cytoplasmic elements. The erythrocytes are lysed and, therefore, do not obscure observation of the elements of interest. This fixative can be used according to the Saccomanno method.

Supplier: VWR Chemicals

VWR® Bouin tissue fixative solution.

Supplier: VWR Chemicals

Specially used for freezing of surgical parts before the cutting process with cryostat or with a freezing microtome.

Supplier: VWR Chemicals

Fixing solution with 0,02% formaldehyde in methanol 50% (v/v)).

Supplier: VWR Chemicals

This medium-acting decalcifier contains a mixture of hydrochloric acids.