You searched for: Western Blotting Reagents

Supplier: Thermo Fisher Scientific

ECL™ Western blotting substrate is an entry-level Western blotting substrate.

Supplier: MOLECULAR DEVICES

The ScanLater™ Western Blot Assay Kits are a time-resolved fluorescence (TRF)-based assay, optimal for quantitating as little as femtogram protein samples.

Supplier: Thermo Fisher Scientific

The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.

Supplier: Thermo Fisher Scientific

Some antibodies are difficult to remove from Western blots and require Longer incubation times or incubation temperatures greater than 22 °C. Restore™ PLUS Western Blot Stripping Buffer was developed to reduce incubation times while keeping incubations at room temperature. High-affinity antibodies can be quickly and effectively stripped from Western blots without removing transferred proteins, thereby allowing multiple reprobes of the target.

Supplier: Merck

Chemiluminescent HRP detection reagent for use on immunoblots, Western blotting, dot/slot blotting.

Supplier: Merck

Immobilon® Block Noise cancelling reagents are designed especially for Western blotting applications. This family of protein-free, ready-to-use buffers has been optimised to reduce background levels when using chemiluminescence, fluorescent, or phosphotyrosine detection.

Supplier: SIGMA ALDRICH MICROSCOPY

It contains p-Phenylenediamine diHCL (1 part) and catechol (2 parts). Commonly referred to as Hanker Yates reagent. Kit component in the Sigma Leukocyte peroxidase (myeloperoxidase) Procedure, 390A.

Supplier: VWR Chemicals

VWR®, Fixing solution for isoelectric focusing in polyacrylamide ge

Supplier: Merck

Western blotting, binding assays, amino acid analysis, N-terminal protein sequencing, dot/slot blotting, glycoprotein visualisation, lipopolysaccharide analysis.

Supplier: Abcam

The ECL Western Blotting Substrate is a highly sensitive, nonradioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots.

Supplier: Merck

Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electrophoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

Supplier: G-Biosciences

The subcellular fractions from liver tissue are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse livers.

Supplier: Rockland Immunochemicals

These accurate and sensitive kits are ideal for Western blotting or dot blotting methods. They offer flexibility with two unique substrates: Bright Blue TMB substrate and Stable Brown DAB substrate. Components are colour-coded for error-free results. With MaxTag™ you can run up to 40 (10×10cm) immunoblots.

Supplier: Merck

Luminata™ Western HRP substrates are a family of premixed, ready to use chemiluminescent reagents for the detection of HRP-based Westerns.

Supplier: Abcam

Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) is a sensitive immunoenzymatic system for detecting antibodies bound to antigens within tissue sections by light microscopy.

Supplier: Cayman Chemical

This Nitrotyrosine BSA can be used as a positive control for immunoblotting experiments using Nitrotyrosine Polyclonal Antibody and Nitrotyrosine Monoclonal Antibody.

Supplier: Enzo Life Sciences

Whole-cell extract of human HL-60 leukemia cells.

Supplier: G-Biosciences

The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with alkaline phosphatase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

Supplier: G-Biosciences

The femtoLUCENT™ PLUS Reagent Kit facilitates the quick and sensitive non-isotopic detection of proteins on transfer membranes.

Supplier: G-Biosciences

The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with horseradish peroxidase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

Supplier: Abcam

Avidin/biotin blocking kit blocks signals from endogenous avidin, biotin and biotin-binding proteins in tissues when used with biotin-based IHC detection. - Cited in over 35 publications - Ideal for tissues rich in biotin (e.g., liver, kidney) - Easy-to-use, quick protocol

Supplier: Abcam

Universal antibody diluent is a PBS formulation at pH 7.3 with protein carrier and preservative that has been developed for stability during long-term storage and for improved antibody performance.

Supplier: G-Biosciences

These are ready to screen, normal tissue, kidney tissue region blots from mouse and rat. The kidney tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse and rat kidney.

Supplier: G-Biosciences

The Swift™ Western Diluent is a solution that reduces the blocking and antibody incubations on Western blot membranes to <60 minutes. Swift™ Western Diluent generates comparable results to traditional Western blotting procedures and other commercial “fast” Western blotting kits.

Supplier: Merck

Immobilon® Western HRP substrates are compatible with both PVDF and nitrocellulose membranes, as well as all commonly used buffers and blocking reagents.

Supplier: Merck

Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

Supplier: Abcam

Very High Sensitivity ECL Substrate Kit () ab133408 uses a new horseradish peroxidase (HRP) substrate specially developed to take advantage of the large linear dynamic range provided by CCD imaging.

Supplier: Abcam

The Optiblot SDS-PAGE Sample Preparation Kit (ab133414) provides a means to quickly concentrate protein samples, and separate them from buffers that interfere with electrophoresis.

Supplier: G-Biosciences

Ready to screen, normal tissue human cell line blots from epithelial, endothelial, skin, and muscle tissue.

Supplier: Enzo Life Sciences

HIGHDEF® red IHC chromogen can be used in conjunction with peroxidase-based immunostaining systems.