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You searched for: Electrophoresis Reagents

Electrophoresis Reagents

Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Diluent for western blotting, Swift™ Western Diluent

Diluent for western blotting, Swift™ Western Diluent

Supplier: G-Biosciences

The Swift™ Western Diluent is a solution that reduces the blocking and antibody incubations on Western blot membranes to <60 minutes. Swift™ Western Diluent generates comparable results to traditional Western blotting procedures and other commercial “fast” Western blotting kits.

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Chemicon® ReBlot Plus Strong Antibody Stripping Solution, 10X

Chemicon® ReBlot Plus Strong Antibody Stripping Solution, 10X

Supplier: Merck

Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

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Nitrotyrosine BSA for immunoblotting experiments

Nitrotyrosine BSA for immunoblotting experiments

Supplier: Cayman Chemical

This Nitrotyrosine BSA can be used as a positive control for immunoblotting experiments using Nitrotyrosine Polyclonal Antibody and Nitrotyrosine Monoclonal Antibody.

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Western blots, pre-made, mouse subcellular fraction specific blot

Western blots, pre-made, mouse subcellular fraction specific blot

Supplier: G-Biosciences

The subcellular fractions from liver tissue are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse livers.

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Luminata™ Western HRP Substrates Samples for Antibodies of All Affinities

Luminata™ Western HRP Substrates Samples for Antibodies of All Affinities

Supplier: Merck

Western blotting, binding assays, amino acid analysis, N-terminal protein sequencing, dot/slot blotting, glycoprotein visualisation, lipopolysaccharide analysis.

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Chemicon® ReBlot Plus Kits

Chemicon® ReBlot Plus Kits

Supplier: Merck

Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electrophoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

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Rat single tissue blot, single species

Rat single tissue blot, single species

Supplier: G-Biosciences

This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.

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Mouse single tissue blot, single species

Mouse single tissue blot, single species

Supplier: G-Biosciences

This blot contains two protein lanes, a protein marker lane and a lane with one GenLysate™ sample. After the proteins are resolved, they are transferred to a PVDF membrane using Western Transfer Buffer. The blots are ready to be blocked and probed with the antibodies of choice.

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Heart tissue region blots, single species

Heart tissue region blots, single species

Supplier: G-Biosciences

These are ready to screen, normal tissue, heart tissue region blots from human and pig. The heart tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult pig and adult human hearts.

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Visualizer and Visualizer EC Western Blot Detection Kits

Visualizer and Visualizer EC Western Blot Detection Kits

Supplier: Merck

The Visualizer Western Blot Detection kit is designed for the sensitive detection of proteins in chemiluminescent Western blots.

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Chemicon® ReBlot Western Blot Recycling Kit

Chemicon® ReBlot Western Blot Recycling Kit

Supplier: Merck

Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

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Brain tissue region blots, single species

Brain tissue region blots, single species

Supplier: G-Biosciences

G-Biosciences offers ready-to-screen, normal tissue, brain tissue region blots. The brain tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse, rat, human, and primate brains.

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Decontamination bags, activated charcoal

Decontamination bags, activated charcoal

Supplier: Biotium

Activated charcoal retains ethidium bromide, GelRed™ and GelGreen™ efficiently in a convenient bag for decontamination of used gel staining solutions. Each bag has a total capacity of at least 10 mg ethidium bromide.

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Chemicon® ReBlot Plus Mild Antibody Stripping Solution, 10X

Chemicon® ReBlot Plus Mild Antibody Stripping Solution, 10X

Supplier: Merck

Western blotting is a commonly used technique for studying protein function and localisation. Typically, protein samples are electro-phoresed on SDS-page and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of southern and northern blots, it has been difficult to reuse western blots.

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Western blots, pre-made, human single tissue single species

Western blots, pre-made, human single tissue single species

Supplier: G-Biosciences

Ready-to-screen, human, single tissue blots, from both normal and tumour tissues. G-Biosciences pre-made blots are prepared with the appropriate GenLysate™ and 50 μg protein is loaded onto a 4 to 20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved, they are transferred to a PVDF membrane, using our Western transfer buffer.

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Transfer pads for western blotting, Swift™

Transfer pads for western blotting, Swift™

Supplier: G-Biosciences

SWIFT™ transfer pads are treated with a proprietary electrolyte buffer to enhance Western blot transfer efficiency.

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Caspase-3 substrates, NucView®

Caspase-3 substrates, NucView®

Supplier: Biotium

NucView® caspase-3 substrates are fluorescent probes that allow for the real-time detection of apoptosis by monitoring caspase-3/7 activity in intact cells using confocal microscopy, flow cytometry or microplate readers.

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NPP ELISA alkaline phosphatase substrate

Supplier: Rockland Immunochemicals

NPP ELISA alcaline phosphatase substrate is a chromogenic substrate used in immunosorbent assays.

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Botanical garden blot

Botanical garden blot

Supplier: G-Biosciences

G-Biosciences' Botanical Garden Blot is prepared using total protein extracted from a variety of 5 to 10 day old, whole plant tissues.

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Peqlab peqGOLD, Protein Marker V (Pre-Stained)

Peqlab peqGOLD, Protein Marker V (Pre-Stained)

Supplier: VWR Chemicals

Protein Marker V (pre-stained) for monitoring protein separation, transfer efficiency and size determination.

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VWR® EZ-Vision®, DNA Ladder, 1 kb

VWR® EZ-Vision®, DNA Ladder, 1 kb

Supplier: VWR Chemicals

EZ-Vision® 1 kb DNA ladder is a pre-mixture of DNA marker, loading buffer and a proprietary fluorescent DNA visualisation dye that can be applied directly to the gel. This non toxic, non mutagenic dye eliminates the need for ethidium bromide staining. The 1 kb ladder consists of 10 double-stranded bands with molecular weights between 200 and 10 000 bp. The intensity of individual bands varies to facilitate easy identification.

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Peqlab peqGOLD, Protein Marker IV (Pre-Stained)

Peqlab peqGOLD, Protein Marker IV (Pre-Stained)

Supplier: VWR Chemicals

peqGOLD pre-stained protein marker IV is a mixture of 10 recombinant, highly purified coloured proteins supplied 'ready to use' in loading buffer. The protein concentrations are optimised to yield well-defined bands directly visible in SDS-polyacrylamide gels.

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Peqlab peqGOLD, DNA Ladder, 100 bp Plus

Peqlab peqGOLD, DNA Ladder, 100 bp Plus

Supplier: VWR Chemicals

peqGOLD DNA ladder 100 bp plus is ideal for size determination in the range of 100 to 3000 base pairs. There are no unspecific bands besides the fragments described. 1000 and 500 bp bands have a higher DNA content and serve as a size reference.

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VWR® PCR DNA Marker

VWR® PCR DNA Marker

Supplier: VWR Chemicals

PCR DNA Marker™ offers 8 fragments ranging from 50 to 2000 bp. The marker contains high intensity reference bands that are ideal for quick sizing of PCR products and restriction digests. Simply mix 10 μl of PCR DNA Marker™ with required quantity of loading buffer and load into well. There is sufficient material for 100 assays.

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VWR®, DNA ladders

VWR®, DNA ladders

Supplier: VWR Chemicals

VWR® DNA ladders are supplied in a loading buffer that is ready to use on agarose and polyacrylamide DNA gels. The ladders are suitable with TBE, TAE, SB and LB electrophoresis systems.

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VWR® RiboReady™, Color RNA Ladder, 1 kb

VWR® RiboReady™, Color RNA Ladder, 1 kb

Supplier: VWR Chemicals

RiboReady™ Color 1 kb RNA ladder allows precise sizing of RNA molecules in denaturing acrylamide gels.

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Peqlab peqGOLD, DNA Ladder, 1 kb

Peqlab peqGOLD, DNA Ladder, 1 kb

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

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VWR®, DNA ladder, 100 bp

VWR®, DNA ladder, 100 bp

Supplier: VWR Chemicals

Ladder with 13 fragments ranging from 100 to 3000 bp for easy band identification.

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VWR® EZ-Vision®, DNA Ladder, 100 bp

VWR® EZ-Vision®, DNA Ladder, 100 bp

Supplier: VWR Chemicals

EZ-Vision® 100 bp DNA ladder is a pre-stained molecular weight ladder in loading buffer that allows immediate visualisation of DNA ladder post-electrophoresis.

   Sustainable Options Available
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Peqlab peqGOLD, DNA Ladders, 50 bp

Peqlab peqGOLD, DNA Ladders, 50 bp

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

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