Protein Extraction Reagents

Supplier: Merck Millipore (Calbiochem‎)

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyses proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Supplier: G-Biosciences

Protein-OUT™ is a washing solution that cleans plastic and glassware used in the proteolytic digestion and peptide isolation for mass spectrometry analysis. The rinsing of plastic and glassware with Protein-OUT™ will remove interfering chemicals and peptides that can leach from the tube walls and interfere or suppress the mass spectrometry signal. In addition, rinsing with Protein-OUT™ removes inadvertent contamination with dust and keratin.

Supplier: Promega

Rabbit Reticulocyte Lysate, Untreated contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors) but has not been treated with micrococcal nuclease. Untreated Lysate is used primarily for the isolation of these components and as an abundant source of endogenous globin mRNA.

Supplier: Promega

rLys-C, MS grade, is a recombinant Lys-C expressed in E. coli. It cleaves at C-terminus of lysines.

Supplier: Promega

FastBreak™ cell lysis reagent is provided as a 10X concentrate and contains a proprietary non ionic detergent to facilitate lysis. Recombinant proteins can be directly screened in the cell extract or purified by the addition of the appropriate affinity matrix such as the MagneHis™ protein purification system.

Supplier: Promega

Arg-C, sequencing grade, is an endopeptidase (clostripain) that cleaves at the C-terminus of arginine residues, including the sites next to proline and it occurs also at proline.

Supplier: Promega

Endoproteinase hydrolyses peptide bonds on the N-terminal side of aspartic and cysteic acid residues (Asp and Cys).

Supplier: Promega

Glu-C sequencing grade serine protease (S. aureus V8) specifically cleaves at the C-terminus of either aspartic or glutamic acid residues.

Supplier: Promega

ProteaseMAX™ Surfactant ensures fast and efficient protein digestion with proteases such as trypsin, chymotrypsin and Lys-C.

Supplier: Promega

Trypsin Gold, mass spectrometry grade, has been manufactured to provide maximum specificity. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion. The specificity of the purified trypsin is further improved by TPCK treatment, which inactivates chymotrypsin. The treated trypsin is then purified by affinity chromatography and lyophilised.

Supplier: Promega

Trypsin/Lys-C mix improves peptide digestion over trypsin alone, increasing peptide recovery and resulting in better protein quantitation by using standard digestion conditions.

Supplier: Promega

Wheat germ extract contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors).

Supplier: Promega

Fast, efficient and ultra-specific cysteine protease for use alone or with other proteases for bottom-up proteomics.

Supplier: VARIAN

Uniform latex particles are used in a wide range of applications that exploit their physico-chemical properties in sophisticated immunoassay formats, both in medicine and veterinary science research.