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Nucleic Acid Reagents

Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.

Cyanine 3-UTP (enhanced)

Cyanine 3-UTP (enhanced)

Supplier: Enzo Life Sciences

Cyanine 3-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP .

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TriLink® CleanAmp® dNTP Mix

TriLink® CleanAmp® dNTP Mix

Supplier: TriLink BioTechnologies

CleanAmp® dNTPs are chemically-modified nucleotides designed to improve specificity, sensitivity, and robustness of your PCR.

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Fragment selection kits, Axygen® AxyPrep™ FragmentSelect-I Kit

Fragment selection kits, Axygen® AxyPrep™ FragmentSelect-I Kit

Supplier: Corning

The AxyPrep™ FragmentSelect Kits utilise a paramagnetic bead technology for quick high throughput optimised DNA size selection suitable for various next generation sequencing platforms. The AxyPrep™ FragmentSelect-I kit is optimised for Illumina next generation sequencing platforms and Life Technologies (SOLiD™) fragment size selection needs simplifying the library construction workflow.

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DpnI, FastDigest™, fermentas

DpnI, FastDigest™, fermentas

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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Restriction enzymes, EcoRI, Fermentas

Restriction enzymes, EcoRI, Fermentas

Supplier: Thermo Fisher Scientific

AATTC sites and cuts best at 37 °C in its own unique buffer. Exhibit 100% activity in the recommended buffer and reaction conditions.

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Restriction enzymes, HindIII, FastDigest™, Fermentas

Restriction enzymes, HindIII, FastDigest™, Fermentas

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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Genomic DNA extraction from dried blood spots, Extracta™ DBS

Genomic DNA extraction from dried blood spots, Extracta™ DBS

Supplier: Quantabio

Extracta™ DBS is a ready to use DNA extraction reagent for rapid and efficient recovery of PCR-ready DNA from dried blood spots (DBS) on Guthrie cards or Whatman® 903 filter/TFN or 226 from Ahlstrom Munksjö paper. This patented single-solution process produces DNA eluates that are substantially free of PCR inhibitors and compatible with a variety of end-point PCR, real-time PCR and Next Generation Sequencing (NGS) or Sanger Sequencing reagents. Application of Extracta DBS with PerfeCTa® qPCR ToughMix® or PerfeCTa MultiPlex qPCR ToughMix® enables accurate and reproducible quantification of DNA sequences in blood using TaqMan® hydrolysis probe real-time qPCR.

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Restriction enzymes, SalI, FastDigest™, Fermentas

Restriction enzymes, SalI, FastDigest™, Fermentas

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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Restriction enzymes, XhoI, FastDigest™, Fermentas

Restriction enzymes, XhoI, FastDigest™, Fermentas

Supplier: Thermo Fisher Scientific

FastDigest™ XhoI is one of an advanced line of fast restriction enzymes that are all 100% active in the universal FastDigest™ and FastDigest™ Green reaction buffers.

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Restriction enzymes, BpiI, FastDigest™, fermentas

Restriction enzymes, BpiI, FastDigest™, fermentas

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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Bio-16-ddUTP dideoxynucleotide pack for 3’-oligonucleotide labeling

Supplier: Enzo Life Sciences

The procedure for labeling of DNA probes with a polynucleotide 'tail' containing hapten-labeled nucleotides was developed by Enzo. In such terminal labeling reactions, terminal transferase catalyzes the addition of nucleotides to any 3'-OH terminus in a template independent manner. This rapid and convenient nonradioactive labeling procedure is free of any sequence bias that is normally observed in random priming or nick translation reactions. Terminal labeling is an ideal procedure for the labeling of oligonucleotides.

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Cytomegalovirus BioProbe®

Supplier: Enzo Life Sciences

The Cytomegalovirus (CMV) BioProbe® labeled probe is prepared by nick translation of cloned fragments of the CMV Towne strain. The fragments include a total of 30-31 kb of DNA, approximately 20% of the CMV genome. The probe is specific for cytomegalovirus DNA. It does not hybridize to DNAs of other herpesviruses (i.e., herpes simplex virus or Epstein-Barr virus) but it has been found to hybridize to the DNA of CMV clinical isolates. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).

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Adenovirus BioProbe®

Supplier: Enzo Life Sciences

The Adenovirus BioProbe® labeled probe is prepared by nick translation of cloned fragments of the adenovirus 5 genome. The fragments constitute a total of 20 kb of DNA, approximately 56% of the genome. The probe is specific for adenovirus and, in addition to hybridization to adenovirus 5 sequences, the probe will cross hybridize with adenovirus types 4, 7, 11, 20, 40 and 41 due to their considerable sequence homology. Fragment size range: 100-1000 base pairs (as estimated by agarose gel electrophoresis).

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Fast DNA™ SPIN kit for faeces, MP Biomedicals

Fast DNA™ SPIN kit for faeces, MP Biomedicals

Supplier: MP Biomedicals

The Fast DNA® SPIN Kit for Faeces quickly and efficiently isolates PCR-ready genomic DNA from fresh or frozen human and animal stool samples.

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Restriction enzymes, NdeI, Fermentas

Restriction enzymes, NdeI, Fermentas

Supplier: Thermo Fisher Scientific

TATG sites and cuts at 37 °C in O buffer (Isoschizomers: FauNDI).

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Restriction enzymes, PstI, FastDigest™, Fermentas

Restriction enzymes, PstI, FastDigest™, Fermentas

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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Restriction enzymes, BsmBI, FastDigest™, fermentas

Restriction enzymes, BsmBI, FastDigest™, fermentas

Supplier: Thermo Fisher Scientific

Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.

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Restriction enzymes, HindIII, 10 U/μl, Fermentas

Restriction enzymes, HindIII, 10 U/μl, Fermentas

Supplier: Thermo Fisher Scientific

HindIII restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions.

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Restriction enzymes, NotI, 10 U/µl, Fermentas

Restriction enzymes, NotI, 10 U/µl, Fermentas

Supplier: Thermo Fisher Scientific

GGCCGC sites and cuts best at 37 °C in O buffer.

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BioProbe® 3’-Oligonucleotide labeling system reagent pack

Supplier: Enzo Life Sciences

The procedure for labeling of DNA probes with a polynucleotide "tail" containing hapten-labeled nucleotides was developed by Enzo. In such terminal labeling reactions, terminal transferase catalyzes the addition of nucleotides to any 3'-OH terminus in a template independent manner. This rapid and convenient nonradioactive labeling procedure is free of any sequence bias that is normally observed in random priming or nick translation reactions. Terminal labeling is an ideal procedure for the labeling of oligonucleotides.

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Restriction enzymes, Restriction enzyme, Eco147I (StuI), Fermentas

Restriction enzymes, Restriction enzyme, Eco147I (StuI), Fermentas

Supplier: Thermo Fisher Scientific

CCT sites and cuts best at 37 °C in B buffer (Isoschizomers: AatI, PceI, SseBI, StuI).

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Restriction enzymes, SfiI, Fermentas

Restriction enzymes, SfiI, Fermentas

Supplier: Thermo Fisher Scientific

NGGCC sites and cuts best at 50 °C in G buffer.

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RNA Purification kits, RNAID® spin kit, MP Biomedicals

Supplier: MP Biomedicals

The RNaid® Spin Kit is designed to isolate and purify RNA (75 bases to 10 kb) from native and denaturing agarose and polyacrylamide gels. The kit includes spin filters which prevent silica particle carry-over into cleaned RNA.

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RNA extraction, MPure™ Total RNA extraction kit , MP Biomedicals

Supplier: MP Biomedicals

MPure™ Total RNA Extraction Kit is used with the MPure-12™ Automated Nucleic Acid Purification System for extraction of total RNA from whole blood, blood cells, animal tissue, plant tissue, yeast or cultured cells and viral RNA from human biological specimens such as serum, plasma, and other cell-free fluids.

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DNA extraction, MPure™ Blood DNA extraction kits, MP Biomedicals

DNA extraction, MPure™ Blood DNA extraction kits, MP Biomedicals

Supplier: MP Biomedicals

MPure™ Blood DNA Extraction Kit is used with the MPure-12™ aNAP System for extraction of DNA from 100 to 400 µl mammalian whole blood, peripheral blood mononuclear cells or buffy coat. Nucleic acids extracted from MPure Blood DNA Extraction Kit 200 can be used in a number of downstream application including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP, Southern Blot Analysis.

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DNA purification kits, Geneclean® kit, MP Biomedicals

DNA purification kits, Geneclean® kit, MP Biomedicals

Supplier: MP Biomedicals

The GeneClean® Kit is designed to purify fragments of DNA from 200 bp to 20 kb from solutions and agarose gels.

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Genomic RNA purification kits, Fast RNA™ Pro Blue kit, MP Biomedicals

Genomic RNA purification kits, Fast RNA™ Pro Blue kit, MP Biomedicals

Supplier: MP Biomedicals

FastRNA® Pro Blue Kit is used to isolate total RNA from gram positive and gram negative bacteria. Isolated RNA is suitable for RT-PCR analysis and other downstream applications.

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Fast RNA™ Pro Soil-Direct Kit, MP Biomedicals

Supplier: MP Biomedicals

FastRNA™ Pro Soil-Direct Kit is used to isolate total RNA from organic material found in soil samples. The total RNA isolated is suitable for RT-PCR analysis and other downstream applications.

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DNA purification kits, GeneClean® spin kit, MP Biomedicals

DNA purification kits, GeneClean® spin kit, MP Biomedicals

Supplier: MP Biomedicals

The GeneClean® Spin Kit is used to purify fragments of DNA from 200 bp to 300 kb from solutions and agarose gels. The kit includes spin filters whose usage prevents silica particle carry-over into cleaned DNA.

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SPINeasy® DNA Kit for Tissue (with Lysing Matrix)

Supplier: MP Biomedicals

SPINeasy® DNA Kit for Tissue (with Lysing Matrix) is a high-performance genomic DNA (gDNA) extraction kit which combines mechanical, chemical and enzymatic disruption of tissue samples in 30 minutes. The addition of proteinase K allows a more efficient and complete digestion of any tissue type. Homogenizing time is greatly reduced while substantially increasing the gDNA yield and purity. The kit is validated for tissue samples of fatty, tough, and fibrous samples, and suitable for downstream applications such as PCR and qPCR.

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