Supplier: Thermo Fisher Scientific

The Verso™ qRT-PCR system combines the Verso™ RT enzyme with ABsolute™ Blue qPCR master mix to provide maximum convenience and versatility. Available in a 1- or 2-step format, as well as multiple pack sizes, to suit all application and throughput requirements.

Supplier: Thermo Fisher Scientific

ABsolute™ qPCR low ROX mixes are optimised for use with real time instruments that are able to detect long wavelength dyes with greater sensitivity, such as the ABI 7500. The lower level of ROX still facilitates normalisation across the plate, while maintaining the sensitivity of the assay. The mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X qPCR mix contains a proprietary reaction buffer that provides high sensitivity, specificity and reproducibility.

Supplier: Promega

T4 DNA Ligase catalyses the joining of two cohesive- or blunt-ended strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides. T4 DNA Ligase is provided with 10X reaction buffer: 300 mM Tris-HCl (pH 7,8 at 25 °C), 100 mM MgCl₂, 100 mM DTT and 10 mM ATP.

Supplier: Promega

T4 DNA Ligase catalyses the joining of two cohesive- or blunt-ended strands of DNA between the 5'-phosphate and the 3'-hydroxyl groups of adjacent nucleotides. T4 DNA Ligase is provided with 10X reaction buffer: 300 mM Tris-HCl (pH 7,8 at 25 °C), 100 mM MgCl₂, 100 mM DTT and 10 mM ATP.

Supplier: Promega

The LigaFast™ rapid DNA ligation system is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just five minutes (blunt-ended inserts in as little as 15 minutes).

Supplier: Promega

T4 RNA ligase catalyses the ATP-dependent ligation of single-stranded RNA or DNA onto the 5'-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient), has an apparent molecular weight of 43,5 kDa. It also catalyses the addition of [5'-³²P] nucleoside 3',5'-bis (phosphate) onto single-stranded RNA.

Supplier: Promega

Useful host for transformation of pGEM Vectors and production of ssDNA from M18 or phagemid vectors. JM109 is recA- and lacks the E. coli K restriction system. Can be used for blue/white screening.

Supplier: Promega

GoTaq® Flexi DNA Polymerase is a proprietary formulation of Taq DNA polymerase that gives robust amplification equal to and in some cases superior to that of standard Taq polymerase.

Supplier: Promega

The GoTaq® qPCR Master Mix is optimised for fast and reproducible quantitative PCR assays using a double-stranded DNA binding dye. The master mix is provided as a ready to use, stabilised 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl₂, dNTPs, BRYT Green® dye and a proprietary reaction buffer.

Supplier: Promega

PCR Master Mix is a ready-to-use 2X solution containing Taq DNA polymerase, dNTPs, MgCl₂ and reaction buffers at optimal concentrations for efficient PCR amplification of DNA templates. The PCR Master Mix is designed for routine endpoint PCR for DNA amplicons in the range of 0,2 to 2 kb.

Supplier: Promega

The GoTaq® 2-Step RT-qPCR system facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components such as GoScript™ reverse transcription system and GoTaq® qPCR master mix. It includes an optimised reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

Supplier: Promega

The GoTaq® 1-Step RT-qPCR system enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR master mix in a single-step real-time amplification reaction. It combines optimised quantities of GoScript™ Reverse Transcriptase, RNasin® Plus RNase inhibitor and additives to enhance single-step reactions.

Supplier: Promega

GoTaq® G2 Flexi DNA Polymerase reliably amplifies a wide range of PCR templates and provides high-performing results due to improved manufacturing processes, increased reliability and consistency.

Supplier: Promega

5X Colourless GoTaq® buffer

Supplier: Promega

The GoTaq® 2-Step RT-qPCR system facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components such as GoScript™ reverse transcription system and GoTaq® qPCR master mix. It includes an optimised reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

Supplier: Promega

GoTaq® Master Mixes are ready-to-use 2X solutions that contain GoTaq® DNA Polymerase, dNTPs, MgCl₂ and reaction buffer at optimal concentrations for efficient amplification of DNA templates by PCR.

Supplier: Promega

GoTaq® Long PCR Master Mix contains the high performance GoTaq® Hot Start Polymerase in a specially formulated mixture with a proprietary thermal stable proofreading polymerase. This optimised enzyme mixture allows efficient amplification of up to 40kb from lambda DNA or 30kb from human genomic DNA.

Supplier: Promega

GoTaq® DNA polymerase is a proprietary formulation of Taq DNA polymerase that gives robust amplification equal to and, in some cases superior to that of standard Taq polymerase.

Supplier: Promega

Use Carboxy-X-Rhodamine (CXR) Reference Dye (identical to ROX™ dye) with instruments that require a higher level of reference dye that than in the GoTaq® qPCR Master Mix. Component of GoTaq® qPCR and RT-qPCR Systems.

Supplier: Promega

High performance GoTaq® G2 DNA Polymerase is bound to a proprietary antibody that blocks polymerase activity. Polymerase activity is restored during the initial denaturation step, when amplification reactions are heated at 94 to 95°C for two minutes, allowing hot-start PCR in which polymerase activity is inhibited at temperatures below 70 °C for convenient, room-temperature reaction set-up.

Supplier: Promega

The GoTaq® Probe qPCR Master Mix is optimised for fast and reproducible quantitative PCR assays in the hydrolysis probe detection format. The master mix is provided as a ready to use, stabilised 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl₂, dNTPs and a proprietary reaction buffer.

Supplier: Promega

GoTaq® Hot Start Master Mix is a premixed ready-to-use solution containing GoTaq® Hot Start Polymerase, dNTPs, MgCl₂ and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR.

Supplier: Promega

GoTaq® G2 Hot Start Master Mix is a premixed ready-to-use solution containing GoTaq® G2 Hot Start Polymerase, dNTPs, MgCl₂ and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR.

Supplier: Promega

GoTaq® G2 Master Mix is a premixed ready-to-use solution containing GoTaq® G2 Polymerase, dNTPs, MgCl₂ and reaction buffers at optimal concentrations for efficient amplification of DNA templates by PCR.

Supplier: Promega

Reliable detection of Legionella pneumophila in water supplies is important to prevent occurrence of Legionnaires’ disease.

Supplier: Promega

GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity.

Supplier: Promega

The GoTaq® 1-Step RT-qPCR system enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR master mix in a single-step real-time amplification reaction. It combines optimised quantities of GoScript™ Reverse Transcriptase, RNasin® Plus RNase inhibitor and additives to enhance single-step reactions.

Supplier: STEMCELL TECHNOLOGIES

Immunomagnetic extraction of total nucleic acid (DNA and RNA).

Supplier: STEMCELL TECHNOLOGIES

For purification of genomic DNA from cells or tissue.

Supplier: STEMCELL TECHNOLOGIES

For purification of RNA from cells.