You searched for: Nucleic Acid Reagents

Supplier: Promega

The HaeIII restriction enzyme is capable of digesting DNA in 15 minutes or less. It recognises the cut site GG^˅CC and CC_˄GG.

Supplier: Promega

G sites and cuts best at 60 °C in its own unique buffer. Exhibit 25 to 50% activity in the recommended buffer and reaction conditions.

Supplier: Promega

G at 37 °C in its own unique buffer.

Supplier: Promega

The XhoI restriction enzymes are capable of digesting DNA in 15 minutes or less. It recognises the cut site C^˅TCGA G and G AGCT_˄C. Genome qualified to ensure optimal performance in genomic analysis applications

Supplier: Promega

CCGG G at 37 °C in its own unique buffer.

Supplier: Promega

G at 37 °C in its own unique buffer.

Supplier: Promega

TG at 37 °C in its own unique buffer.

Supplier: Promega

A sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

A sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

The HaeIII restriction enzyme is capable of digesting DNA in 15 minutes or less. It recognises the cut site GG^˅CC and CC_˄GG.

Supplier: Promega

GoScript™ Reverse Transcriptase system utilises the M-MLV reverse transcriptase enzyme for reliable cDNA synthesis of a full range of rare and abundant transcripts over a wide range of RNA input amounts (5 µg to 1 pg). The components of the system can effectively reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA. Optimised for use in qPCR, including with GoTaq® Real-Time PCR Systems, it can handle the most challenging templates and is resistant to PCR inhibitors.

Supplier: Promega

The AccessQuick™ RT-PCR system is an easy and convenient master mix for one-tube RT-PCR. It increases the convenience of performing RT-PCR by combining the following components in a single tube, such as Tfl DNA polymerase, dNTPs, magnesium sulphate and reaction buffer. The AMV RT enzyme is provided in a separate tube to allow important no-RT control reactions. Master mix is simply added to RNA templates in reaction vials, followed by the AMV RT, primers and water. It is intended for routine RT-PCR applications that have been previously optimised and do not require extreme conditions.

Supplier: BIOPREMIER

SUPREME RT-PCR detection kit HLVd is designed for the detection of Hop Latent Viroid (HLVd) in a single PCR reaction. The kit is suitable for use in real time instruments with FAM and Cy5 detection channels.

Supplier: PRIMERDESIGN

The oasig freeze drying process stabilises all of the active components allowing these reagents to be shipped and stored at room temperature. This hugely simplifies the logistics of purchasing, shipping and using the technology. Whether you are in a sophisticated laboratory in Texas or a mobile field hospital in Timbuktu we can supply complete qPCR kit and reagent packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.
Freeze dried qPCR reagents stored and shipped at ambient temperatures:

Supplier: Promega

CG sites and cuts best at 37 °C in its own unique buffer.

Supplier: BIOPREMIER

This Supreme RT-PCR detection kit HLVd/LCV/CanCV is designed for the detection of Hop Latent Viroid (HLVd), Lettuce Chlorotic Virus (LCV) and Cannabis Cryptic Virus (CanCV) in a single PCR reaction (TETRAPLEX).

Supplier: PRIMERDESIGN

This genesig® Easy qPCR detection kit targets the invasion protein (invA) gene from Salmonella spp. The kit is designed for the in vitro quantification of Salmonella spp genomes and to have a broad detection profile.

Supplier: Promega

Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5 kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71 kDa. The RNase H activity of M-MLV RT is weaker than that of the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Supplier: Promega

Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long messenger RNA templates (>5 kb). The enzyme is a product of the pol gene of M-MLV and consists of a single subunit with a molecular weight of 71 kDa. The RNase H activity of M-MLV RT is weaker than that of the commonly used Avian Myeloblastosis Virus (AMV) reverse transcriptase.

Supplier: Promega

The Viability PCR Reagent System uses a proprietary molecule that irreversibly modifies nucleic acid so that it cannot be amplified. This molecule is cell- and capsid-impermeable, enabling selective detection of nucleic acid from only live cells or intact viruses.

Supplier: Promega

T3 RNA Polymerase is a DNA-dependent RNA polymerase that synthesises RNA using only T3 DNA or DNA templates cloned downstream from a T3 promoter. The polymerase can incorporate ³²P, ³³P, ³H and ³⁵S nucleoside triphosphates during transcription.

Supplier: Thermo Fisher Scientific

ABsolute™ qPCR Mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers. Versions are available for use with probes or with SYBR® Green I included, and with or without ROX or Fluorescein passive reference dyes.

Supplier: Thermo Fisher Scientific

ABsolute™ qPCR Capillary Mixes contain an optimised reaction buffer specifically designed to provide the highest possible sensitivity, specificity and consistent end-point values in real time instruments that use glass capillaries, such as the Roche LightCycler®. The 2X mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes.

Supplier: Promega

SP6 RNA Polymerase is a DNA-dependent RNA polymerase used for in vitro transcription. Only SP6 DNA or DNA cloned downstream from an SP6 promoter can be used as a template for SP6 RNA Polymerase-directed RNA synthesis. The polymerase can incorporate ³²P, ³³P, ³H and ³⁵S nucleoside triphosphates.

Supplier: Promega

T7 RNA Polymerase is a DNA-dependent RNA polymerase specific for T7 promoter sequences, that exhibits extremely high specificity for its cognate promoter sequences. Only T7 DNA or DNA cloned downstream from a T7 promoter can serve as a template for T7 RNA Polymerase-directed RNA synthesis.

Supplier: Cytiva

illustra™ Hot Start Mix, Ready-To-Go™ is a pre-formulated and pre-dispensed, freeze-dried PCR reagent mix, including high quality PuReTaq™ DNA polymerase and a hot start activator protein, for increased specificity and reproducibility of PCR amplifications. The Ready-To-Go™ beads are provided in either 0,5 or 0,2 ml tubes that are compatible with most thermal cyclers.

Supplier: Thermo Fisher Scientific

ABsolute™ qPCR low ROX mixes are optimised for use with real time instruments that are able to detect long wavelength dyes with greater sensitivity, such as the ABI 7500. The lower level of ROX still facilitates normalisation across the plate, while maintaining the sensitivity of the assay. The mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers/probes. The 2X qPCR mix contains a proprietary reaction buffer that provides high sensitivity, specificity and reproducibility.

Supplier: Cytiva

illustra™ Hot Start Master Mix is a 2X premixed formulation that can effectively reduce non specific priming and primer-dimer formation during PCR. illustra™ Hot Start Master Mix uses a novel hot start method called primer sequestration.

Supplier: Thermo Fisher Scientific

The Verso™ qRT-PCR system combines the Verso™ RT enzyme with ABsolute™ Blue qPCR master mix to provide maximum convenience and versatility. Available in a 1- or 2-step format, as well as multiple pack sizes, to suit all application and throughput requirements.

Supplier: Thermo Fisher Scientific

The Verso™ RT-PCR system combines a reverse transcription enzyme, improved priming options and optimised buffers to generate high yield and full length cDNA. With a variety of kit options for both 1- and 2-step RT-PCR reactions, Verso™ provides optimum flexibility and sensitivity in an easy to use format.