You searched for: Nucleic Acid Reagents

Supplier: Promega

The Access RT-PCR system is designed for the reverse transcription (RT) and PCR amplification of a specific target RNA from total RNA or mRNA. The system uses AMV reverse transcriptase (AMV RT) from Avian Myeloblastosis virus for first-strand DNA synthesis and thermostable Tfl DNA polymerase from Thermus flavus for second-strand cDNA synthesis and DNA amplification. Included is an optimised single-buffer system that permits extremely sensitive detection of RNA transcripts without a requirement for buffer additions between the reverse transcription and PCR amplification steps. This simplifies the procedure and reduces the potential for contaminating the samples.

Supplier: Promega

The Reverse Transcription system provides reagents to efficiently reverse transcribe RNA into cDNA. The cDNA prepared from each reaction using this system may be used directly in multiple PCR amplifications using Taq DNA polymerase. The AMV Reverse Transcriptase synthesises single-stranded cDNA from total or poly(A)+ RNA. Both Oligo(dT)15 and random primers are included, allowing cDNA synthesis from virtually any RNA source. The system contains sufficient reagents for 100 cDNA synthesis reactions, processing 1 μg of RNA per reaction. Each cDNA synthesis reaction may be divided and used in up to 20 separate PCR amplifications.

Supplier: Promega

G at 37 °C in its own unique buffer.

Supplier: Promega

(T/C)TG at 37 °C in its own unique buffer.

Supplier: Promega

CCGG G at 37 °C in its own unique buffer.

Supplier: Promega

CATG G sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

GoScript™ Reverse Transcriptase system utilises the M-MLV reverse transcriptase enzyme for reliable cDNA synthesis of a full range of rare and abundant transcripts over a wide range of RNA input amounts (5 µg to 1 pg). The components of the system can effectively reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA. Optimised for use in qPCR, including with GoTaq® Real-Time PCR Systems, it can handle the most challenging templates and is resistant to PCR inhibitors.

Supplier: Promega

G at 37 °C in its own unique buffer.

Supplier: Promega

The TaqI restriction enzyme recognises the cut site T^˅CG A and A GC_˄T.

Supplier: Promega

TAA sites and cuts best at 37 °C in its own unique buffer. Exhibit 100% activity in the recommended buffer and reaction conditions.

Supplier: Promega

These SmaI restriction enzymes are genome qualified to ensure optimal performance in genomic analysis applications. Recognises CCC^˅GGG and GGG_˄CCC.

Supplier: Promega

C sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

C sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

G sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

TA GCG sites and cuts best at 37 °C in its own unique buffer. Star activity may be observed with glycerol concentrations >12% or with enzyme: DNA ratios >25 U/μg.

Supplier: Promega

AC sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

Oligo (dT)₁₅ primer is suitable for first-strand cDNA synthesis with a reverse transcriptase. The primer hybridises to the poly(A) tail of mRNA. Random hexamers are primers used for first-strand cDNA synthesis and cloning.

Supplier: Promega

G at 37 °C in its own unique buffer.

Supplier: Promega

TAG at 37 °C in its own unique buffer.

Supplier: Promega

The XhoI restriction enzymes are capable of digesting DNA in 15 minutes or less. It recognises the cut site C^˅TCGA G and G AGCT_˄C. Genome qualified to ensure optimal performance in genomic analysis applications

Supplier: Promega

T sites and cuts best at 50 °C in its own unique buffer. Exhibit 10 to 25% activity in the recommended buffer and reaction conditions.

Supplier: Promega

CGA sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

These DdeI restriction enzyme recognises the cut site C^˅TNA G and G ANT_˄C. Produces an ambiguous extension, which is difficult to ligate.

Supplier: Promega

These DpnI restriction enzyme recognises the cut size of G^meA^˅TC and CT_˄^meAG. Requires N6-methylation of the adenine residue for activity.

Supplier: Promega

The ImProm-II™ reverse transcription system produces efficient, robust synthesis of first-strand cDNA in preparation for PCR amplification. The components can be used to reverse transcribe RNA templates starting with total RNA, poly(A)+ mRNA or synthetic transcript RNA. The optimised reaction buffer and ImProm-II™ Reverse Transcriptase provided in the system together enable robust, full-length cDNA synthesis for the reproducible analysis of rare or long messages. The cDNA synthesis conditions were formulated for standalone applications or for easy transition to gene-specific target amplification. An aliquot of the reverse transcription reaction (1 to 20 μl) can be amplified directly using Taq DNA polymerase in coupled or uncoupled PCR.

Supplier: Promega

The Xbal restriction enzyme is capable of digesting DNA in 15 minutes or less. It recognises T^˅CTAG A and A GATC_˄T. Genome qualified to ensure optimal performance in genomic analysis applications.

Supplier: Promega

A at 37 °C in its own unique buffer.

Supplier: Promega

CATG G sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

T sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

GTAC sites and cuts best at 37 °C in its own unique buffer.