You searched for: Nucleic Acid Reagents

Supplier: MP Biomedicals

Taq-&LOAD™ PCR Master Mix is an optimised 5X reaction mix for PCR containing recombinant Taq DNA polymerase, dNTPs, MgCl₂, and incubation and loading buffers. Also included are a compound increasing the density of the sample and a red/purple dye.

Supplier: Promega

The DNA polymerase I large (Klenow) fragment mini kit provides polymerase and dNTPs ready to use in a variety of applications.

Supplier: Promega

dNTP Mix is a solution containing sodium salts of dATP, dCTP, dGTP and dTTP, each at 10 mM in water at pH 7,5. The total concentration of nucleotides is 40 mM. One microliter of the dNTP Mix in a 50 μl reaction will give a final dNTP concentration of 200 μM for each dNTP.

Supplier: Promega

The Ribo m⁷G Cap Analog is a modified ribonucleotide with the structure (m⁷G(5´)ppp(5´)G). This methylated ribonucleotide can be incorporated onto the 5´-end of transcripts synthesised in vitro and simulates the 7-methyl guanosine 5´-cap structure found on most eukaryotic mRNA molecules.

Supplier: Promega

Ribonucleotide triphosphates (rNTPs) compatible with the Riboprobe® and HeLaScribe® systems.

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

Supplier: MP Biomedicals

The Taq CORE kits are designed for the amplification of specific regions of DNA using PCR. The PCR CORE Kits - Direct Loading provide Taq DNA Polymerase, PCR Direct Loading buffers and dNTPs necessary for amplification and subsequent loading of samples on an agarose gel.

Supplier: Promega

High-quality deoxynucleotide triphosphates (dNTPs) are important for key procedures including cDNA synthesis, sequencing, cloning, and preparation of libraries for next generation sequencing.

Supplier: G-Biosciences

Taq DNA Polymerase is a highly thermostable recombinant DNA polymerase derived from the thermophile Thermus aquaticus. The molecular weight of the recombinant protein is 94 kD. The Taq Polymerase is able to amplify DNA up to 5 kb with an elongation velocity of 0,9 to 1,2 kb/min at 70 to 75 °C. The error rate of this Taq Polymerase is ~2,2×10⁻⁵ nucleotide⁻¹ cycle⁻¹. Taq DNA polymerase catalyses the 5’→3’ synthesis of DNA. The enzyme has no detectable 3’→5’ proofreading exonuclease activity, and possesses low 5’→3’ exonuclease activity, which results in a 3’-dA overhang on the PCR product

Supplier: MP Biomedicals

Taq-&GO™ is a ready to use optimised reaction mix for PCR.

Supplier: Promega

The T7 RiboMAX™ Express RNAi system is an in vitro transcription system designed for producing milligram amounts of double-stranded RNA (dsRNA) in a short amount of time. The dsRNA is free of protein and other contaminants and is suitable for use in RNA interference (RNAi) in both mammalian and non mammalian systems.

Supplier: Promega

The SP6 and T7 promoter primers are designed for sequencing inserts cloned into the pGEM® Vectors.

Supplier: Promega

The pUC/M13 primers are used to sequence inserts cloned into the M13 vectors and pUC plasmids developed by Messing. These primers also can be used for sequencing other lacZ-containing plasmids such as the pGEM®-Z and pGEM®-Zf Vectors. The primers are purified by gel electrophoresis or HPLC.

Supplier: Promega

G at 37 °C in its own unique buffer.

Supplier: Promega

(T/C)TG at 37 °C in its own unique buffer.

Supplier: Promega

CCGG G at 37 °C in its own unique buffer.

Supplier: Promega

CATG G sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

G at 37 °C in its own unique buffer.

Supplier: Promega

The TaqI restriction enzyme recognises the cut site T^˅CG A and A GC_˄T.

Supplier: Promega

TAA sites and cuts best at 37 °C in its own unique buffer. Exhibit 100% activity in the recommended buffer and reaction conditions.

Supplier: Promega

These SmaI restriction enzymes are genome qualified to ensure optimal performance in genomic analysis applications. Recognises CCC^˅GGG and GGG_˄CCC.

Supplier: Promega

C sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

C sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

G sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

TA GCG sites and cuts best at 37 °C in its own unique buffer. Star activity may be observed with glycerol concentrations >12% or with enzyme: DNA ratios >25 U/μg.

Supplier: Promega

AC sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

Oligo (dT)₁₅ primer is suitable for first-strand cDNA synthesis with a reverse transcriptase. The primer hybridises to the poly(A) tail of mRNA. Random hexamers are primers used for first-strand cDNA synthesis and cloning.

Supplier: Promega

G at 37 °C in its own unique buffer.

Supplier: Promega

TAG at 37 °C in its own unique buffer.

Supplier: Promega

The XhoI restriction enzymes are capable of digesting DNA in 15 minutes or less. It recognises the cut site C^˅TCGA G and G AGCT_˄C. Genome qualified to ensure optimal performance in genomic analysis applications