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Nucleic Acid Reagents

Nucleic Acid Reagents

Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.

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PerfeCTa® PreAmp 5X SuperMix

PerfeCTa® PreAmp 5X SuperMix

Supplier: Quantabio

PerfeCTa® PreAmp SuperMix is a 5X concentrated, ready to use reaction cocktail for unbiased, selected enrichment of target sequences from limited amounts of starting material for downstream gene expression profiling or targeted resequencing. It contains all components, except primers and templates. The 5X concentrated SuperMix allows addition of higher template volumes when working with low concentration samples, and/or reduced reaction volumes. Inclusion of an inert light blue tracer dye helps visualise small reaction volumes and ensure accurate pipetting.

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Genomic DNA purification kit, Fermentas

Genomic DNA purification kit, Fermentas

Supplier: Thermo Fisher Scientific

DNA purification kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.

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Restriction enzymes, NotI, 50 U/µl, Fermentas

Restriction enzymes, NotI, 50 U/µl, Fermentas

Supplier: Thermo Fisher Scientific

GGCCGC sites and cuts best at 37 °C in O buffer.

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AMPIGENE® qPCR 1-Step Probe Kit Lo-ROX

Supplier: Enzo Life Sciences

Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.

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AccuStart™ master mixes, long range SuperMix

AccuStart™ master mixes, long range SuperMix

Supplier: Quantabio

Superior sensitivity and multiplexing for DNA amplification of long targets.

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T7 RNA polymerase

Supplier: Thermo Fisher Scientific

T7 RNA polymerase is ideal for synthesis of unlabeled and labeled RNA. It catalyses the 5'→3' synthesis of RNA on either single-stranded DNA or double-stranded DNA downstream from it promoter. It is also used in the study of RNA secondary structure and RNA-protein interactions, RNA splicing.

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TriLink® Engineered RNase Inhibitor

TriLink® Engineered RNase Inhibitor

Supplier: TriLink BioTechnologies

Engineered RNase Inhibitor is a protein to inhibit RNase activities to prevent RNA degradation in IVT.

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Tissue DNA kit, genomic DNA isolation from tissue, E-Z 96®

Tissue DNA kit, genomic DNA isolation from tissue, E-Z 96®

Supplier: OMEGA BIO-TEK

By adapting HiBind® technology with 96-well plates, the E-Z 96® Tissue DNA kit provides a high throughput method to purify genomic DNA from whole blood, buccal swabs, mouse tail, rat tail, tissues and animal cells and tissues in a 96-well plate format. Purified DNA is suitable for most downstream applications such as PCR, restriction enzyme digestion, and hybridisation techniques.

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Mag-Bind® Plant DNA DS 96 kit

Supplier: OMEGA BIO-TEK

The Mag-Bind® Plant DNA DS 96 Kit is designed for difficult samples (DS) which are hard to lyse or contain high amounts of polysaccharides and polyphenols. The system uses a CTAB-based lysis buffer which does not require organic solvents. The proprietary binding system prevents inhibitors from binding to the magnetic beads. The wash buffer system further removes polysaccharides, phenolic compounds and enzyme inhibitors from plant tissue lysate. An optional rebinding step is included with the kit but most samples do not require the additional treatment.

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DNA Library Prep Kit, sparQ

DNA Library Prep Kit, sparQ

Supplier: Quantabio

An optimised kit for the rapid construction of DNA libraries from fragmented double-stranded DNA for sequencing on Illumina® NGS platforms and prepares for sequencing success with the highest quality library.

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Montage® SEQ96 Sequencing Reaction Cleanup Kits

Montage® SEQ96 Sequencing Reaction Cleanup Kits

Supplier: Merck

The Montage® SEQ96 sequencing reaction cleanup kit provides the filtration plate and solution necessary to remove contaminating salts and unincorporated dye terminators from DNA sequencing reactions.

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Viral RNA Xpress kit, Mag-Bind®

Viral RNA Xpress kit, Mag-Bind®

Supplier: OMEGA BIO-TEK

Isolate viral RNA from nasopharyngeal swab specimens (dry or in VTM) using magnetic beads.

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qScript™ XLT One-Step RT-qPCR ToughMix®

qScript™ XLT One-Step RT-qPCR ToughMix®

Supplier: Quantabio

qScript™ XLT One-Step RT-qPCR ToughMix® is a ready to use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates.

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PerfeCTa® qPCR ToughMix®

Supplier: Quantabio

PerfeCTa® qPCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues or animal tissues. It is a versatile and robust Real-Time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR ToughMix® contains all required reaction components, except primers, probe(s) and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.

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DNA mini columns for genomic/plasmid DNA extraction, Hi-Bind®

Supplier: OMEGA BIO-TEK

Hi-Bind® DNA mini columns are silica glass fibre columns that can bind up to 100 μg of genomic DNA or 35 μg of plasmid DNA per prep. Columns feature an attached lid and a vacuum luer for processing with vacuum manifolds and by centrifugation.

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sparQ PureMag beads

sparQ PureMag beads

Supplier: Quantabio

sparQ PureMag beads is a fast and reliable nucleic acid purification system for reaction cleanup and size selection in Next Generation Sequencing (NGS) workflows. Based on the reversible nucleic acid-binding properties of magnetic beads; this product can be used to quickly remove primers, primer-dimers, unincorporated nucleotides, salts, adapters and adapter-dimers from NGS library prep reactions to improve downstream sequencing performance. sparQ PureMag beads allows excellent recovery of fragments greater than 100 bp without centrifugation or filtration. Consistent and reliable size selection can be achieved by simply adjusting the beads to sample ratio. This product is designed for both manual and automated processing, allowing seamless integration into existing workflows.

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