Electroforese, Tampões

Os tampões de eletroforese mantêm níveis de pH consistentes durante a separação de DNA, RNA e outras proteínas. Fornecendo iões que transferem a corrente aplicada, as soluções de baixa carga permitem que moléculas de diferentes tamanhos viajem a várias velocidades para análises. Oferecendo proteção adicional às proteínas, as substâncias preparativas previnem a degradação, garantindo a viabilidade das amostras e resultados precisos. Disponíveis em diferentes pesos moleculares, o melhor tampão de eletroforese pode ser selecionado com base nas macromoléculas específicas em análise.

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Phosphate Buffer, pH 6.6 at 25 °C

Supplier: SIGMA ALDRICH MICROSCOPY

Phosphate buffer's buffering capacity is pH sensitive and is preferred to be used at physiological pH (7,2 to 7,4).

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VWR® Transfer Buffer Concentrate, CAPS 10X

Supplier: VWR Chemicals

For transfer of protein to membranes for sequencing or blotting.

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VWR®, MOPS 10X buffer for biotechnology

Supplier: VWR Chemicals

MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.

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VWR® UltraPure, Tris-glycine buffers, 10X concentrate

Supplier: VWR Chemicals

Running buffers for both 1-D and 2-D protein gel electrophoresis.

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VWR® Buffer, MES-SDS, 20X, UltraPure Grade

Supplier: VWR Chemicals

Useful for high resolution of proteins on neutral pH SDS-PAGE gels.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Millipore®

Supplier: Merck

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Tampão fosfato salino (PBS), baixo teor de fosfatos

Supplier: VWR Chemicals

Solução irradiada para utilização em lavagem de células.

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VWR® TBE Buffer Solution, 10X Concentrate (Tris-Borate-EDTA Buffer), UltraPure Grade

Supplier: VWR Chemicals

Running Buffer, TBE buffer solution 10X concentrate (Tris-borate-EDTA buffer)

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VWR® Proteomics Grade, Tris-glycine buffer

Supplier: VWR Chemicals

Um pó de Tris-Glicina pre-misturado para preparação de corrida de Laemmli SDS_PAGE. A embalagem de 40 L prepara 40 Litros de solução 1X Tris-glicina (0.025 M Tris base e 0.192 M Glicina). Cada Ready-Pack™ prepara 1 L de uma solução 10X concentrada.

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VWR® UltraPure, Tris-acetate-EDTA (TAE) buffer

Supplier: VWR Chemicals

TAE is an extensively used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE buffer is more compatible with in-gel manipulations and band recovery procedures than TBE buffer.

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VWR®, solução salina tamponada com fosfato (PBS), baixo teor de fosfato, 10X

Supplier: VWR Chemicals

Concentrado de PBS irradiado, adequado para uso em lavagem de células após diluição.

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Tampão fosfato salino (PBS), com alto teor de fosfatos

Supplier: VWR Chemicals

Solução irradiada, pH de 7,2, para lavagem de blots.

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VWR®, Hybridisation buffers with 50% formamide

Supplier: VWR Chemicals

Hybridisation cocktail offering blocking and hybridisation for optimised complimentary base pairing of DNA and RNA in Southern blot, Northern blot, dot blot, colony and in situ hybridisation.

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VWR®, SSC buffer, 20X, ultra-pure grade

Supplier: VWR Chemicals

SSC buffer is a hybridisation buffer for Northern and Southern blots.

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VWR® Separating Gel Buffer, 4X

Supplier: VWR Chemicals

4X concentrated buffer solution (1,5 M Tris, pH 8,8 and 0,4% SDS). Used for preparation of standard acrylamide:bis-acrylamide gels as well as preparation of gels using PAGE-PLUS™ acrylamide solution.