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You searched for: Protein Purification

Protein Purification

Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.

Protease affinity chromatography media, PreScission™, Cytiva

Protease affinity chromatography media, PreScission™, Cytiva

Supplier: Cytiva

Enables the low-temperature cleavage of fusion proteins containing the PreScission Protease recognition sequence.

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Affinity chromatography media, IMAC Sepharose™ 6 Fast Flow

Affinity chromatography media, IMAC Sepharose™ 6 Fast Flow

Supplier: Cytiva

IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.

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Gel filtration media, Sephadex™ G-15

Gel filtration media, Sephadex™ G-15

Supplier: Cytiva

Sephadex™ G-15 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1500 molecular weight.

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Affinity chromatography media, IgG Sepharose™ 6 Fast Flow

Affinity chromatography media, IgG Sepharose™ 6 Fast Flow

Supplier: Cytiva

lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose 6 Fast Flow matrix, with human IgG covalently coupled to it.

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SDS-PAGE Clean Up Kit

SDS-PAGE Clean Up Kit

Supplier: Cytiva

SDS-PAGE clean up kit is designed to prepare samples for SDS-PAGE that are frequently difficult to analyse due to the presence of salts or low protein concentration.

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Ion exchange chromatography media, SP Sepharose™ High Performance

Ion exchange chromatography media, SP Sepharose™ High Performance

Supplier: Cytiva

SP Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with sulphopropyl (SP) strong cation exchange groups.

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Superose™ 6 Gel Filtration Media, Prep Grade

Superose™ 6 Gel Filtration Media, Prep Grade

Supplier: Cytiva

Superose™ 6 PG for separations of proteins and other biomolecules according to size. Wide separation in two molecular weight ranges from 1000 up to 5 million.

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Ion exchange chromatography media, IEX Sepharose™ Fast Flow

Ion exchange chromatography media, IEX Sepharose™ Fast Flow

Supplier: Cytiva

Q Sepharose Fast Flow is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades.

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Ion exchange chromatography media, Capto™ S ImpAct

Ion exchange chromatography media, Capto™ S ImpAct

Supplier: Cytiva

Capto™ S ImpAct is a strong cation exchange chromatography medium (resin) developed for polishing of monoclonal antibodies (MAbs) and other biomolecules.

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SP Sepharose™ XL Ion Exchange Chromatography Media

SP Sepharose™ XL Ion Exchange Chromatography Media

Supplier: Cytiva

SP Sepharose™ XL is a strong cation exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.

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Ion exchange chromatography media, SOURCE™ 15Q

Ion exchange chromatography media, SOURCE™ 15Q

Supplier: Cytiva

Mono-sized 15 um rigid beads gives low back pressure and high flow rates allowing for high productivity.

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Affinity chromatography media, MabSelect SuRe™ LX

Affinity chromatography media, MabSelect SuRe™ LX

Supplier: Cytiva

MabSelect SuRe™ LX is a protein A affinity medium with very high dynamic binding capacity at extended residence times and is developed for high titer antibody processes.

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Sepharose™ 4B Gel Filtration Base Matrix

Sepharose™ 4B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ 4B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.

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Gel filtration media, Sephadex™ G-50

Gel filtration media, Sephadex™ G-50

Supplier: Cytiva

Sephadex™ G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.

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Affinity coupling media, CNBr-activated Sepharose™ 4

Affinity coupling media, CNBr-activated Sepharose™ 4

Supplier: Cytiva

CNBr-activated Sepharose™ 4 Fast Flow is based on the Sepharose™ Fast Flow platform. The resin is composed of cross-linked 4% agarose beads that has been pre-activated with cyanogen bromide.

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Sepharose™ 4 FF Octyl Fast Flow Hydrophobic Interaction Chromatography Media

Sepharose™ 4 FF Octyl Fast Flow Hydrophobic Interaction Chromatography Media

Supplier: Cytiva

Octyl Sepharose™ 4 Fast Flow resin is based on cross-linked 4% agarose. The aliphatic ligand is immobilised to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.

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Reversed phase chromatography media, SOURCE™ 15RPC

Reversed phase chromatography media, SOURCE™ 15RPC

Supplier: Cytiva

SOURCE™ 15RPC is designed for reversed phase chromatography (RPC) with full pH range stability and suitable for polishing steps in industrial processes requiring high flow velocities and low back-pressure.

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Gel filtration media, Sephadex™ G-100

Gel filtration media, Sephadex™ G-100

Supplier: Cytiva

Sephadex™ G-100 is useful for desalting and buffer exchange of very large biomolecules. Sephadex™ G-100 Superfine is designed for specific cases requiring very high resolution.

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Ion exchange chromatography media, CM Sepharose™ Fast Flow

Ion exchange chromatography media, CM Sepharose™ Fast Flow

Supplier: Cytiva

CM Sepharose™ Fast Flow is a weak cation exchanger based on the well established Sepharose™ Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

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Plasmid purification media, PlasmidSelect Xtra

Plasmid purification media, PlasmidSelect Xtra

Supplier: Cytiva

PlasmidSelect Xtra is a thiophilic aromatic adsorption chromatography medium with a selectivity that allows generic purification of supercoiled covalently closed circular forms of plasmid DNA to be separated from open circular forms.

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Affinity chromatography media, MabSelect™

Affinity chromatography media, MabSelect™

Supplier: Cytiva

MabSelect™ is a protein A affinity resin designed for capturing monoclonal antibodies (mAbs) from large sample volumes.

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Affinity chromatography media, Benzamidine Sepharose™ 4 Fast Flow (High Sub)

Affinity chromatography media, Benzamidine Sepharose™ 4 Fast Flow (High Sub)

Supplier: Cytiva

Benzamidine Sepharose™ 4 Fast Flow (High Sub) is a tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as zymogens, including urokinase and prekallikrein.

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Con A Sepharose™ 4B Affinity Chromatography Media, Cytiva

Supplier: Cytiva

Con A Sepharose is Concanavalin A coupled to Sepharose 4B by the cyanogen bromide method.

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Gel filtration media, Superdex™ 30 PG

Gel filtration media, Superdex™ 30 PG

Supplier: Cytiva

Superdex prep grade is a preparative gel filtration medium with a composite matrix of dextran and agarose

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CM Sephadex™ C-25 Ion Exchange Chromatography Media

CM Sephadex™ C-25 Ion Exchange Chromatography Media

Supplier: Cytiva

Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.

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Sepharose™ CL-6B Gel Filtration Base Matrix

Sepharose™ CL-6B Gel Filtration Base Matrix

Supplier: Cytiva

Sepharose™ CL-6B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.

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Hydrophobic interaction chromatography media, Butyl Sepharose™ 4 Fast Flow

Hydrophobic interaction chromatography media, Butyl Sepharose™ 4 Fast Flow

Supplier: Cytiva

Butyl Sepharose 4 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.

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Ion exchange chromatography media, DEAE Sephacel™

Ion exchange chromatography media, DEAE Sephacel™

Supplier: Cytiva

DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of ~10000 kDa for globular proteins. Supplied pre-swollen in 20% ethanol.

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Affinity chromatography media, MabSelect SuRe™

Affinity chromatography media, MabSelect SuRe™

Supplier: Cytiva

MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.

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Affinity coupling media, Epoxy-Activated Sepharose™ 6B

Supplier: Cytiva

Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.

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