You searched for: Protein Purification Resins

Supplier: Cytiva

Sephadex™ G-75 is well established gel filtration medium for desalting and buffer exchange of large biomolecules >80 000 molecular weight.

Supplier: Cytiva

Good resolution separations of proteins and other biomolecules according to size, Wide separation range – separations in two molecular weight ranges from 1,000 up to 5 million, Excellent reproducibility and durability

Supplier: Cytiva

Capto™ L is a bioprocessing chromatography resin for the capture of antibodies and antibody fragments. It combines a rigid, high-flow agarose matrix with the immunoglobulin-binding recombinant protein, L ligand, which has a strong affinity to the variable region of antibody kappa light chains. It is suitable for the capture of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies (Dabs).

Supplier: Cytiva

SP Sephadex™ C-25 is a strong cation exchanger. The ion exchange group is a sulphopropyl group which remains charged and maintains consistently high capacity over the entire working range of pH 4 to 13.

Supplier: Cytiva

DEAE Sepharose™ Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform. It is composed of cross-linked 6% agarose beads, with diethylaminoethyl (DEAE) weak anion exchange groups. Scale-up with DEAE Sepharose™ Fast Flow is straight forward.

Supplier: Cytiva

The 2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution.

Supplier: Cytiva

Ni Sepharose™ excel is an IMAC medium pre-charged with nickel ions very strongly bound to a chelating ligand. Samples that usually cause stripping of metal ions can, therefore, be loaded to the medium. Ni Sepharose™ excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.

Supplier: Promega

The Maxwell® 16 Polyhistidine Protein Purification Kit is used with the Maxwell® 16 instrument to provide an easy method for the efficient, automated purification of polyhistidine-tagged protein from bacterial cultures and other sample types including mammalian and insect cells. With minor modifications, the reagents can also be used for purification of HQ-tagged proteins from bacterial cultures.

Supplier: Promega

The MagneGST™ protein purification system provides a simple, rapid and reliable method for the purification of glutathione-S-transferase (GST) fusion proteins. Immobilised glutathione paramagnetic particles are used to isolate GST-tagged protein directly from a crude or cleared E. coli lysate using either a manual or automated procedure.

Supplier: Promega

The MagneHis™ protein purification system provides a simple, rapid, reliable method for the purification of polyhistidine- or HQ-tagged, expressed proteins. Paramagnetic pre-charged nickel particles are used to isolate polyhistidine- or HQ-tagged protein directly from a crude E. coli cell lysate using either a manual or automated procedure.

Supplier: Promega

SoftLink™ resin is a rigid, methacrylate polymeric gel filtration matrix, functionalised with covalently bound, monomeric avidin. Used for the isolation and purification of biotinylated molecules.

Supplier: Merck

Chelex® 100 Chelating Resin is a weak cation exchange resin that has high selectivity, approximately 5,000:1, for copper, iron, and other heavy metal ions over monovalent cations. Chelex 100 Resin is made of a styrene divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions.

Supplier: Merck

Sepharose Fast Flow is composed of macroporous, beaded, cross-linked agarose and modified with sulphopropyl (SP) strong cation exchange groups which remains charged and maintains consistently high capacity over the entire working range of pH 4 to 13.