You searched for: Protein Extraction Kits

Supplier: Thermo Fisher Scientific

Imject™ Maleimide-Activated Blue Carrier™ Protein enables conjugation of a sulfhydryl-containing hapten to elicit an immune response and antibody production against the hapten. Blue Carrier™ Protein is a haemocyanin from the Concholepas concholepas mollusc with immunogenic properties similar to keyhole limpet haemocyanin. Blue Carrier™ Protein has a large mass (~8 × 106 Da) and has been successfully used in polyclonal and monoclonal antibody production for
synthetic haptens and peptides.

Supplier: Merck Millipore (Calbiochem‎)

Native endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyses amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease: protein by weight) for sequence analysis.

Supplier: Promega

The Flexi® rabbit reticulocyte lysate system allows translation reactions to be optimised for a wide range of parameters, including Mg²⁺ and K⁺ concentrations and the choice to add or omit DTT.

Supplier: Promega

The TNT® Coupled Wheat Germ Extract Systems offer an alternative for eukaryotic cell-free protein expression. Standard wheat germ extract translations commonly use RNA synthesised in vitro from SP6 or T7 RNA polymerase promoters. This entire process requires separate reactions with several steps between each reaction. The TnT® Extracts bypass many of these steps by incorporating transcription directly in the translation mix. Additionally, the TnT® Extract reactions often produce significantly more protein in a 1.5-hour reaction than do standard in vitro wheat germ extract translations using RNA templates.

Supplier: Promega

Rabbit Reticulocyte Lysate System, Nuclease Treated can be used to identify mRNA species, characterise their protein products and investigate transcriptional and translational control.