Protein Expression Systems
Protein expression systems make it very easy to sensitively detect various proteins used widely in bioanalytics and molecular biology. Fluorophores or certain vitamins are used to tag antibodies or other proteins, and these labelled molecules can then be purified or detected using other solutions such as streptavidin or monomeric avidin. These protein expression systems are optimized for the rapid synthesis of functionally active proteins.
Cell-free protein expression kits, 1-step human coupled IVT kit - DNA
Supplier: Thermo Fisher Scientific
The 1-Step Human Coupled IVT Kit – DNA is a mammalian in vitro translation (IVT) system based on HeLa cell lysates. The kit contains all of the cellular components required for protein synthesis, including ribosomes, initiation factors, elongation factors and tRNA. When supplemented with the included proprietary accessory proteins, reaction mix and a DNA template cloned into the Thermo Scientific pT7CFE1-based vector, this system can synthesise protein for up to 6 hours. The kit includes a pT7CFE-CHis vector for ease of use in downstream applications.
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Cell-free protein expression kits, 1-step human high yield mini IVT kit
Supplier: Thermo Fisher Scientific
The Thermo Scientific 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems.
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Pierce™ Cell-free Protein Expression Kits, 1-Step Human High Yield Maxi IVT Kit
Supplier: Thermo Fisher Scientific
The 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per ml than other mammalian IVT systems.
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Autoinduction systems, Overnight Express™ Instant LB and TB Media
Supplier: Merck Millipore (Novagen)
The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.
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Autoinduction systems, Overnight Express™ NMR Media
Supplier: Merck Millipore (Novagen)
The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.
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Rapid mouse antibody isotyping kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Rapid isotyping kit is a 5-minute lateral-flow assay with ELISA sensitivity for class and subclass determination of monoclonal antibodies.
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Autoinduction systems, Overnight Express™ Autoinduction System 2
Supplier: Merck Millipore (Novagen)
The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.
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Autoinduction systems, Overnight Express™ Autoinduction System 1
Supplier: Merck Millipore (Novagen)
The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.
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CFXpress™ E.coli Cell-free Protein Synthesis Kit
Supplier: Genscript
The GenScript CFXpress™ Cell-free E. coli Protein Synthesis Kit (CFPS) is a ready-to-use platform for rapid, high-yield in vitro protein expression from DNA or mRNA templates under the control of a T7 promoter.
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High yield protein expression system, S30 T7
Supplier: Promega
S30 T7 high yield protein expression system can be used to express up to 500 μg/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site.
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Quick coupled transcription/translation system, TnT®
Supplier: Promega
Quick Coupled Transcription/Translation System provides convenient, single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression.
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Coupled Reticulocyte Lysate Systems, TnT®
Supplier: Promega
TnT® Coupled Reticulocyte Lysate System offers researchers an alternative for eukaryotic cell-free protein expression. It greatly simplifies the process and reduces the time required to obtain in vitro translation results.
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T7 insect cell extract protein expression system, TNT®
Supplier: Promega
The TnT® T7 Insect Cell Extract Protein Expression System is a convenient, quick, single-tube, coupled transcription/translation system for the cell-free expression of proteins using an extract derived from the Spodoptera frugiperda Sf21 cell line.
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T7 quick protein expression system, for PCR, TNT®
Supplier: Promega
The TnT® T7 Quick for PCR is a simple, single-tube coupled transcription/translation system designed for optimum protein expression directly from PCR templates.
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Regulated mammalian expression system
Supplier: Promega
Regulated mammalian expression system is based on a novel on/off switch that relies on the rapid and sensitive modulation by coumarin-related compounds of a chimeric transactivator protein.
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High yield wheat germ protein expression system, TNT® SP6
Supplier: Promega
The TNT® SP6 high yield wheat germ protein expression system contains optimised wheat germ extract in a single-tube, coupled transcription/translation system that allows you to express proteins in only two hours from plasmid or PCR-generated templates.



