Enzyme Assays

Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.

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Amplite® Cholesterol Quantitation Kit

Supplier: AAT Bioquest

Cholesterol is required to build and maintain cell membranes.

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Amplite® Fluorimetric Alkaline Phosphatase Assay Kit Blue Fluorescence

Supplier: AAT Bioquest

Alkaline phosphatase is a highly sensitive enzyme for ELISA, immuno-histochemical, Northern, Southern and Western blot applications.

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Amplite® Fluorimetric Glucose-6-Phosphate Assay Kit

Supplier: AAT Bioquest

Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.

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Amplite® Gaussia Luciferase Reporter Gene Assay Kit Bright Glow

Supplier: AAT Bioquest

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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MMP Green™ Substrate

Supplier: AAT Bioquest

The matrix metalloproteinases (MMPs) constitute a family of zinc-dependent endopeptidases that function within the extracellular matrix.

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Amplite® Renilla Luciferase Reporter Gene Assay Kit Bright Glow

Supplier: AAT Bioquest

Common reporter genes include beta-galactosidase, beta-glucuronidase and luciferase.

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Amplite® Fluorimetric Glycerol Assay Kit

Supplier: AAT Bioquest

Glycerol is a precursor for the synthesis of triglycerides and phospholipids in liver and adipose tissue.

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Amplite® Fluorimetric Total NADP and NADPH Assay Kit Red Fluorescence

Supplier: AAT Bioquest

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Fluorimetric Lipase Activity Assay Kit

Supplier: AAT Bioquest

Designed for consistent and dependable results. The Amplite® Fluorimetric Lipase Activity Assay Kit provides a simple and quick protocol for measuring lipase activity.

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Amplite® Colorimetric Hexokinase Assay Kit

Supplier: AAT Bioquest

Designed for consistent and dependable results. The Amplite® Colorimetric Hexokinase Assay Kit is designed to accurately measure the activity of hexokinases from various sources using a simple and quick protocol.

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Amplite® Fluorimetric L-Lactate Dehydrogenase (LDH) Assay Kit

Supplier: AAT Bioquest

Lactate dehydrogenase (LDH) is an oxidoreductase enzyme that catalyses the interconversion of pyruvate and lactate.

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Amplite® Colorimetric NADPH Assay Kit

Supplier: AAT Bioquest

Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.

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Amplite® Rapid Colorimetric Total Protein Thiol Quantitation Assay Kit

Supplier: AAT Bioquest

Protein thiols are very important to protein structure, protein function and biological system redox environment.

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Amplite® Colorimetric Hydrogen Peroxide Assay Kit

Supplier: AAT Bioquest

Hydrogen peroxide (H2O2) is a reactive oxygen metabolic by-product that serves as a key regulator for a number of oxidative stress-related states.

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Amplite® Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit Red Fluorescence

Supplier: AAT Bioquest

Horseradish Peroxidase (HRP) is a small molecule (MW ~40 KD) that is widely used in a variety of biological detections.

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Gaussia-firefly luciferase dual assay kit, Pierce™, Thermo Scientific

Supplier: Thermo Fisher Scientific

The Thermo Scientific Pierce Gaussia-Firefly Luciferase dual assay kit provides the necessary reagents to simultaneously detect intracellular Gaussia and red firefly luciferase activity in mammalian whole cell lysates.