You searched for: Enzyme Assays
Either measuring substrate consumption or overall production, the enzyme assays are used to monitor enzymatic activity. Provided with the required supplies and reagents for any preferred method, enzyme kinetics and inhibition studies can be successfully completed. While continuous assay sampling methods give activity readings throughout development, discontinuous techniques calculate after the reaction has been stopped. The standardized enzyme assays will provide accurate identification and measurement of the protein molecules present in samples.
Amplite® Fluorimetric Fluorescamine Protein Quantitation Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Fluorescamine is intrinsically non-fluorescent but reacts rapidly with primary aliphatic amines, including those in peptides and proteins, to yield a blue-green-fluorescent derivative.
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Screen Quest™ Fluorimetric Neutrophil Elastase Inhibitor Screening Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. The Screen Quest™ fluorimetric neutrophil elastase inhibitor screening kit offers a fast, sensitive, and high-throughput method for screening and characterising potential inhibitors of NE.
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Amplite® Colorimetric Glycerol Assay Kit
Supplier: AAT Bioquest
Glycerol is a precursor for synthesis of triglycerides and phospholipids in the liver and adipose tissue.
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Amplite® Colorimetric BCA Protein Quantitation Assay Kit
Supplier: AAT Bioquest
The traditional BCA protein assay is widely used for quantifying proteins.
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Amplite® Colorimetric Glucose-6-Phosphate Assay Kit
Supplier: AAT Bioquest
Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells.
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Amplite® Fluorimetric Caspase 3/7 Assay Kit *Blue Fluorescence*
Supplier: AAT Bioquest
Caspases play important roles in apoptosis and cell signaling.
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Amplite® Fluorimetric L-Lactate Assay Kit
Supplier: AAT Bioquest
Lactic acid is chiral and has two optical isomers: L-lactic acid and D-lactic acid.
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Amplite® Fluorimetric Sphingomyelin Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Sphingomyelin (SM) is largely found in the exoplasmic leaflet of the cell membrane, primarily in nervous tissue.
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Amplite® Colorimetric Glucose Quantitation Kit
Supplier: AAT Bioquest
Glucose, a monosaccharide, is the most important carbohydrate in biology.
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Amplite® Colorimetric Butyrylcholinesterase Activity Assay Kit
Supplier: AAT Bioquest
Butyrylcholinesterase (EC 3.1.1.8; BChE), also known as pseudocholinesterase or plasma cholinesterase, is mainly synthesised in liver and present in blood.
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Amplite® Colorimetric Aspartate Aminotransferase (AST) Assay Kit
Supplier: AAT Bioquest
Aspartate aminotransferase (AST), also called serum glutamic oxaloacetic transaminase (GOT), is a member of transferase family.
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Amplite® Fluorimetric Alanine Aminotransferase Assay Kit
Supplier: AAT Bioquest
Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family.
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Amplite® Colorimetric Oxaloacetate Assay Kit *Red Color*
Supplier: AAT Bioquest
Oxaloacetate is an important part of citric acid cycle, where it reacts with Acetyl-CoA to form citrate.
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Amplite® Fluorimetric Formaldehyde Quantitation Kit *Green Fluorescence*
Supplier: AAT Bioquest
Formaldehyde is a naturally occurring substance.
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Amplite® Colorimetric Alanine Aminotransferase Assay Kit *Blue Color*
Supplier: AAT Bioquest
Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family.
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Amplite® Colorimetric Total NAD and NADH Assay Kit
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NAD⁺) and nicotinamide adenine dinucleotide phosphate (NADP⁺) are two important cofactors found in cells.
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Amplite® Fluorimetric NADPH Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Amplite® Colorimetric NAD/NADH Ratio Assay Kit
Supplier: AAT Bioquest
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells.
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Yeast β-galactosidase assay kit, Pierce™
Supplier: Thermo Fisher Scientific
Yeast β-Galactosidase Assay Kit is specially formulated for yeast cells and ideal for identifying protein interactions in vivo using two-hybrid systems.
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Amplite® Fluorimetric Glutamate Oxidase Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Glutamate oxidase belongs to the family of oxidoreductases, specifically those acting on the CH-NH₂ group of donors with oxygen as an acceptor.
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Amplite® Fluorimetric Hypochlorite (Hypochlorous Acid) Assay Kit
Supplier: AAT Bioquest
Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems.
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Amplite® Fluorimetric Glutathione GSH/GSSG Ratio Assay Kit *Green Fluorescence*
Supplier: AAT Bioquest
When cells are exposed to increased levels of oxidative stress, GSSG will accumulate and the ratio of GSH to GSSG will decrease.
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Amplite® Rapid Colorimetric Protein Aldehyde Content Quantitation Kit
Supplier: AAT Bioquest
Site-selective modification of proteins is of major interest in chemical biology.
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Amplite® Fluorimetric Mercuric Ion Quantitation Kit
Supplier: AAT Bioquest
Mercuric ion is an essential metal ion that plays an important role in a number of biological processes.
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Amplite® Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*
Supplier: AAT Bioquest
Horseradish Peroxidase (HRP) is a small molecule (MW ~40 Kd) that is widely used in a variety of biological detections.
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Amplite® Choline Quantitation Kit
Supplier: AAT Bioquest
Choline and its metabolites play an important role in the structural integrity and signaling of cell membranes and cholinergic neurotransmission (acetylcholine synthesis).
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ReadiUse™ NADP Regenerating Kit
Supplier: AAT Bioquest
Designed for consistent and dependable results. AAT Bioquest's ReadiUse™ NADP regenerating kit provides a flexible prepackaged NADP regenerating system to accommodate various experimental designs.
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Amplite® Colorimetric Aldehyde Quantitation Kit *Blue Color*
Supplier: AAT Bioquest
The formation, reactivity and toxicity of aldehydes originating from the peroxidation of lipids of cellular membranes have received great attention in recent years.
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Amplite® Blue Peroxidase Substrate
Supplier: AAT Bioquest
A variety of substrates are available for detecting peroxidase activity in ELISA assays. colourimetric HRP substrates (e.g., TMB, OPD and ABTS) have been widely used for years. Each of these substrates varies greatly with respect to its performance characteristics such as detection sensitivity, working range and attainable signal-to-noise ratios. Amplite® Blue peroxidase substrate is a soluble fluorogenic substrate for detection of peroxidase activity. Amplite® Blue allows rapid HRP detection assays to be performed with greater sensitivity than the colourimetric HRP substrates (such as TMB and OPD). The fluorescent product of Amplite® Blue does not photobleach. Amplite® Blue exhibits a flat baseline in assays, which facilitates low-level detection sensitivity and allows for high signal-to-noise ratios.
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Amplite® Colorimetric Sphingomyelinase Assay Kit *Blue Color*
Supplier: AAT Bioquest
Sphingomyelinase (SMase) is an enzyme that is responsible for cleaving sphingomyelin (SM) to phosphocholine and ceramide.
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Supplier: GRANT INSTRUMENTS
Supplier: BOCHEM
Supplier: VWR Collection
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Supplier: DWK Life Sciences
Supplier: Thermo Fisher Scientific
Supplier: VWR Collection
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Supplier: Industrial Physics
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Supplier: VWR Collection
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Supplier: Greiner Bio-One