You searched for: Cellular Assays

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 70,000 MW, and contains a formaldehyde-fixable free-amine group.

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 250,000 MW, and contains a formaldehyde-fixable free-amine group.

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 10000 MW, and contains a formaldehyde-fixable free-amine group.

Supplier: ENZO LIFE SCIENCES

This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy.

Supplier: MOLECULAR DEVICES

The FLIPR® Calcium 6 and 6-QF Assay Kits provide a fast, simple, and reliable fluorescence based assay for detecting changes in intracellular calcium. The Calcium 6 Assay Kit significantly reduces fluorescence background with a one-step protocol. The Calcium 6-QF Assay Kit provides a quench-free option for sensitive targets or multiplexing applications.

Supplier: ENZO LIFE SCIENCES

MITO-ID® membrane potential detection kit monitors energetic status using a simple mix-and-read, no-wash protocol. It measures mitochondrial membrane potential with a cationic dye that fluoresces either green or orange depending upon membrane potential status.

Supplier: ENZO LIFE SCIENCES

For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.

Supplier: MOLECULAR DEVICES

SpectraMax® Quant™ dsDNA Assay kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations.

Supplier: Biotium

Apoptosis and necrosis are two processes by which cells die. Apoptosis is an active, regulated disassembly of the cell from within. During apoptosis, phosphatidylserine is translocated from the inner to the outer surface of the cell, allowing the dying cell to be engulfed by phagocytic cells. Annexin V is a 35 kD Ca2+-dependent phospholipid binding protein with a high affinity for phosphatidylserine.

Supplier: Cayman Chemical

Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.

Supplier: Biotium

This NucView™488 and RedDot™2 Apoptosis and Necrosis Assay Kit contains NucView™488 caspase-3 substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.

Supplier: Biotium

The Bacterial Viability and Gram Stain Kit contains three components: CF™488A-WGA, DAPI, and EthD-III solutions for distinguishing between gram-negative and gram-positive, as well as live versus dead bacteria.

Supplier: Biotium

Flow Cytometry Fixation/Permeabilisation Kit contains optimally formulated buffers for fixation and permeabilisation of suspension cells for immunofluorescence staining of intracellular antigens for analysis by flow cytometry. The bufffers are also available separately.

Supplier: Biotium

RedDot™2 is a far-red cell membrane-impermeant nuclear dye similar to Draq7™. Ideal for staining the nuclei of dead or fixed and permeabilized cells with minimal cytoplasmic RNA staining.

Supplier: ENZO LIFE SCIENCES

GFP-Certified™ Apoptosis/Necrosis Detection Kit is a multiplex assay that distinguishes between healthy, early apoptotic, late apoptotic and necrotic cells, compatible with GFP and other green fluorescent probes.

Supplier: MOLECULAR DEVICES

The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. The assay kit is optimised for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis.

Supplier: ENZO LIFE SCIENCES

The kit is designed to directly monitor real time reactive oxygen species (ROS) production and Specifically Superoxide in live cells using fluorescence microscopy or flow cytometry.

Supplier: Biotium

TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyses the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry.

Supplier: MOLECULAR DEVICES

The FLIPR® Membrane Potential Assay Kit provide a fast, simple and reliable, fluorescence-based assay for detecting changes in voltage across the cell membrane and its homogenous fluorescence-based formulations for observation of real-time membrane potential changes associated with ion channel activation and ion transporter proteins.

Supplier: ENZO LIFE SCIENCES

A Fluor-de-Lys® fluorescent assay system. The SIRT2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.

Supplier: MOLECULAR DEVICES

The Fura-2 QBT™ Calcium Kit eliminates the cause of data variability and reduces the number of steps compared to conventional wash protocols using Fura-2.

Supplier: ENZO LIFE SCIENCES

Kit utilises Live-dye™, a cell permeable green fluorescent dye (Ex(max): 488 nm; Em(max): 518 nm), to stain live cells. Dead cells can be easily stained by propidium iodide (PI), a red fluorescent dye (Ex(max): 488 nm; Em(max): 615 nm), which in viable cells is actively pumped out of the cytoplasm.

Supplier: MOLECULAR DEVICES

The FLIPR® Calcium 5 Assay Kit provides a fast, simple, and reliable fluorescence-based assay for detecting changes in intracellular calcium.

Supplier: MOLECULAR DEVICES

The FLIPR® Potassium Assay Kit measures functional activity of ligand- and voltage-gated potassium channels. The homogeneous, no-wash assay protocol provides a large signal window and high Z' values.

Supplier: Biotium

MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.

Supplier: ENZO LIFE SCIENCES

LYSO-ID® Green Detection Kit is an acidic organelle-selective dye specially designed to use with RFP-expressing cell lines, as well as cells expressing blue, cyan or orange fluorescent proteins (BFPs, CFPs, OFPs).

Supplier: MOLECULAR DEVICES

The FLIPR® Calcium 4 Assay Kit provides a fast, simple and reliable fluorescence-based assay for detecting changes in intracellular calcium.

Supplier: ENZO LIFE SCIENCES

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from polyunsaturated fatty acids, are unstable and decompose to form a complex series of compounds. These include reactive aldehydes, of which the most abundant is malondialdehyde (MDA). Therefore, measurement of malondialdehyde is widely used as an indicator of lipid peroxidation. Increased levels of lipid peroxidation products have been associated with a variety of chronic diseases in both humans and model systems.

Supplier: ENZO LIFE SCIENCES

The HDAC3/NCOR1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC3 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.

Supplier: Biotium

ExoBrite™ WGA EV staining kits were designed to overcome some of the challenges of EV detection, particularly in flow cytometry. ExoBrite™ WGA EV stains bind to molecules in the EV membrane for bright, specific staining, with little background.