Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.
Green detection kit, LYSO-ID®
Supplier: ENZO LIFE SCIENCES
LYSO-ID® Green Detection Kit is an acidic organelle-selective dye specially designed to use with RFP-expressing cell lines, as well as cells expressing blue, cyan or orange fluorescent proteins (BFPs, CFPs, OFPs).
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ApoSENSOR™ ADP/ATP ratio assay kit
Supplier: ENZO LIFE SCIENCES
The assay is designed to detect ADP/ATP ratio. The changes in ADP/ATP ratios have been used to conveniently differentiate apoptosis from necrosis.
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Red hydrogen peroxide assay kit
Supplier: ENZO LIFE SCIENCES
Red Hydrogen Peroxide Assay Kit provides a simple absorbance-based or fluorometric assay to detect and quantify hydrogen peroxide or peroxidase activity in biochemical assays, cell extracts and in live cells. It can also be used to detect a variety of oxidase activities through enzyme-coupled reactions. The kit is an optimized ‘mix and read’ assay that is suitable for both 96- and 348-well formats and is compatible with HTS liquid handling.
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Comet SCGE assay kit
Supplier: ENZO LIFE SCIENCES
COMET SCGE Assay measures DNA damage by fluorescently detecting the integrity of DNA liberated from cells embedded in low melting point agarose. Upon electrophoresis, fragmented DNA produces a characteristic "comet" shaped tail as small DNA fragments migrate in the gel more rapidly than in-tact genomic DNA.
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LEADING LIGHT® Wnt cell line assay reagents
Supplier: ENZO LIFE SCIENCES
For use with the LEADING LIGHT® Wnt Reporter Cell Line.
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Epigenetics Screening Library
Supplier: Cayman Chemical
For screening a variety of epigenetic compounds.
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Calcium assay kits, FLIPR® Calcium 4 assay kit
Supplier: MOLECULAR DEVICES
The FLIPR® Calcium 4 Assay Kit provides a fast, simple and reliable fluorescence-based assay for detecting changes in intracellular calcium.
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FLUOR DE LYS® HDAC3/NCOR1 fluorometric drug discovery kit
Supplier: ENZO LIFE SCIENCES
The HDAC3/NCOR1 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC3 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.
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APO-BRDU™ kit, TUNEL Assay
Supplier: Tonbo Biosciences
The APO-DIRECT™ reagent kit provides a two color TUNEL (Terminal deoxynucleotide transferase dUTP Nick End Labelling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry. The kit contains the instructions and reagents required for measuring apoptosis in cells including: positive and negative control cells for assessing reagent performance; washing, reaction and rinsing buffers for processing individual steps in the assay; terminal deoxynucleotidyl tranferase enzyme (TdT), fluorescein isothiocyanate (FITC) deoxyuridine triphosphate (FITC-dUTP) for labeling DNA breaks and PI/RNase A solution for counter staining the total DNA.
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LEADING LIGHT® Wnt cell line medium pack
Supplier: ENZO LIFE SCIENCES
For use with the LEADING LIGHT® Wnt Reporter Cell Line.
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Apoptosis assay kit (Dual), NucView™ 488
Supplier: Biotium
NucView® 488 and CF™dye (594, 640R and Texas RED) conjugated annexin V provides two apoptosis events, caspase-3 activity and phosphatidylserine (PS) translocation.
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ApoSENSOR™ Cell viability assay kit
Supplier: ENZO LIFE SCIENCES
The assay utilizes bioluminescent detection of the ATP level for a rapid screening of apoptosis and cell proliferation simultaneously in mammalian cells. The assay utilizes the enzyme luciferase to catalyze the formation of light from ATP and luciferin. The light can be measured using a luminometer or Beta Counter. The assay can be fully automatic for high throughput (10 seconds/sample) and is highly sensitive (detects 10-100 mammalian cells/well).
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Nuclear-ID™ red cell cycle kit, GFP-CERTIFIED®, for flow cytometry
Supplier: ENZO LIFE SCIENCES
GFP-Certified™ Nuclear-ID™ Red Cell Cycle Analysis Kit provides a convenient approach for studying the induction and inhibition of cell cycle progression by flow cytometry.
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CF® Dye Dextran 150000 MW, anionic and fixable
Supplier: Biotium
CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 150,000 MW, and contains a formaldehyde-fixable free-amine group.
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Black IHC chromogen (HRP), HIGHDEF®
Supplier: ENZO LIFE SCIENCES
HIGHDEF® black IHC chromogen (HRP) is intended to be used in conjunction with horseradish peroxidase (HRP)-based immunostaining or in situ hybridisation systems.
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HIF-1α transcription factor assay Kit
Supplier: Cayman Chemical
HIF-1α Transcription Factor Assay Kit is a sensitive, non radioactive method for detecting HIF-1α from whole cell lysates.
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GFP-CERTIFIED® FLUOFORTE® Calcium assay kit
Supplier: ENZO LIFE SCIENCES
The GFP-Certified™ FluoForte™ Calcium Assay kit provides a homogeneous fluorescence-based assay for detecting intracellular calcium mobilization across a broad spectrum of biological targets.
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Catalase fluorometric detection kit
Supplier: ENZO LIFE SCIENCES
The Catalase fluorometric detection kit provides a higher throughput sensitive method for measuring catalase activity in just one hour. This is a homogeneous mix-and-read assay that enables you to monitor multiple time points for a kinetic read. Positive control is provided.
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CV-Caspase 3 & 7 Detection kit
Supplier: ENZO LIFE SCIENCES
Based on the dye cresyl violet and the caspase 3/7 DEVD peptide recognition sequence, this kit allows the detection of caspases 3 and 7 in living cells by fluorescence microscopy.
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Glutathione Cell-Based Detection Kit (Blue Fluorescence)
Supplier: Cayman Chemical
For rapid and easy determination of GSH in whole cells.
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Apoptosis inducer set
Supplier: G-Biosciences
Apoptosis inducers are used for induction of apoptosis in cultured cells. The selection of apoptosis inducers include actinomycin D, camptothecin, cycloheximide, dexamethasone, doxorubicin and etoposide.
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FLUOR DE LYS® SIRT3 fluorometric drug discovery assay kit
Supplier: ENZO LIFE SCIENCES
A Fluor-de-Lys® fluorescent assay system. The SIRT3 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human SIRT3 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions.
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Prostaglandin Screening Library III
Supplier: Cayman Chemical
For A and J series prostaglandin analog screening.
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Calcium assay kits, FLIPR® Calcium 5 assay kit
Supplier: MOLECULAR DEVICES
The FLIPR® Calcium 5 Assay Kit provides a fast, simple, and reliable fluorescence-based assay for detecting changes in intracellular calcium.
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CF® Dye Dextran 40000 MW, anionic and fixable
Supplier: Biotium
CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 40,000 MW, and contains a formaldehyde-fixable free-amine group.
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Millicell® µ-Migration Assay Kit
Supplier: Merck
This Kit overcomes the limitations of traditional multiwell migration assays. Its microfluidic, low-volume technology promotes a stable, diffusion-generated concentration gradient that is consistently linear and lasts for more than 48 hours.
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Cell integrity kit, EarlyTox™
Supplier: MOLECULAR DEVICES
The EarlyTox™ Cell Integrity Kit enables the differentiation of live cells from dead cells via fluorescent labeling. This is useful for the rapid quantification of cell viability when used with the SpectraMax® MiniMax™ 300 Imaging Cytometer or other fluorescence-based cellular imaging instruments.
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LEADING LIGHT® Wnt reporter cell line
Supplier: ENZO LIFE SCIENCES
Wnt reporter cell line for discovering Wnt pathway modulators.
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FLUOR DE LYS® HDAC fluorometric activity assay kit
Supplier: ENZO LIFE SCIENCES
The FLUOR DE LYS® HDAC fluorometric activity assay kit is a sensitive and convenient alternative to protocols utilizing radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. It is based on the unique FLUOR DE LYS® (Fluorimetric Histone deAcetylaseLysyl) substrate and developer combination and provides an assay that can be carried out in two simple mixing steps, all on the same 96-well plate. Histone deacetylase inhibitors have shown promise as anti-tumor agents and naturally this has stimulated interest in the screening of compounds for HDAC inhibition.
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Cell viability assay kits, EarlyTox™ live/dead assay kits
Supplier: MOLECULAR DEVICES
EarlyTox™ Cell Viability Assay Kits are a family of fluorescence-based reagents for the assessment of cell viability, cell proliferation, and various apoptosis events using mammalian cells. Optimised for use with microplate readers, these assay kits employ a no-wash, homogeneous assay protocol that enables characterisation of a full concentration-response profile of test compounds.