Supplier: Promega

The RealTime-Glo™ MT Cell Viability Assay is a non lytic, homogeneous, bioluminescent method to measure cell viability in real time using a simple, plate-based method. RealTime-Glo™ MT Cell Viability Assay determines the number of viable cells in culture by measuring the reducing potential of cells and thus metabolism (MT).

Supplier: Promega

The BacTiter-Glo™ Microbial Cell Viability Assay is used for determining the number of viable microbial cells in culture based on quantitation of the ATP present. This homogeneous assay involves adding a single reagent, BacTiter-Glo™ Reagent, directly to bacterial cells cultured in medium and measuring luminescence.

Supplier: Promega

The Kinase-Glo® Max Luminescent Kinase Assays are homogeneous non-radioactive methods for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction. The Kinase-Glo® Max Assay is linear to 500 μm ATP, making it well suited for use with kinases with high Km for ATP, as well as for screening for kinase inhibitors that do not compete at the ATP binding site.

Supplier: Promega

The Caspase-Glo® 9 assay is a homogeneous luminescent assay that measures caspase-9 activities. The assay provides a proluminescent caspase-9 substrate in a buffer system optimised for caspase-9 activity, luciferase activity and cell lysis. The addition of a single Caspase-Glo® 9 reagent results in cell lysis, followed by caspase cleavage of the substrate and generation of 'glow-type' luminescent signal. The signal is proportional to caspase-9 activity.

Supplier: Promega

The Caspase-Glo® 3/7 assay is a homogeneous luminescent assay that measures caspase-3/7 activities. The assay provides a proluminescent caspase-3/7 DEVD-aminoluciferin substrate and a proprietary thermostable luciferase in a reagent optimised for caspase-3/7 activity, luciferase activity and cell lysis. This liberates free aminoluciferin, which is consumed by the luciferase, generating a 'glow-type' luminescent signal. The signal is proportional to caspase-3/7 activity. It is designed for use with multiwell plate formats using either purified enzyme or cells in culture.

Supplier: Promega

PDE-Glo™ Phosphodiesterase Assay is a luminescent, high-throughput screening (HTS) method for measuring cyclic nucleotide phosphodiesterase (PDE) activity from purified sources. The PDE-Glo™ Assay is used for screening library compounds to identify inhibitors of either cAMP- or cGMP-specific PDEs. It is optimised to work with both cAMP- and cGMP-dependent phosphodiesterases.

Supplier: Promega

Pgp-Glo™ Assay Systems provide the necessary reagents to perform luminescent P-glycoprotein (Pgp) ATPase assays. Pgp, also known as MDR1 and ABCB1, is a 170 kDa integral plasma membrane protein that functions as an ATP-dependent drug efflux pump and plays an important role in multidrug resistance and certain adverse drug-drug interactions. Compounds that interact with Pgp can be identified as stimulators or inhibitors of its ATPase activity. Compounds that are substrates for transport by Pgp typically stimulate its ATPase activity.

Supplier: Promega

The PepTag® Non-Radioactive Protein Kinase Assay Systems use brightly coloured fluorescent peptide substrates that are highly specific for PKC (PepTag® C1 Peptide-PLSRTLSVAAK) and PKA (PepTag® A1 Peptide-LRRASLG). Phosphorylation of the peptide alters the net charge from +1 to –1, allowing phosphorylated and non phosphorylated versions of the substrate to be rapidly separated on an agarose gel at neutral pH.

Supplier: Promega

The ROS-Glo™ H₂O₂ Assay is a homogeneous, fast and sensitive bioluminescent assay that measures the level of hydrogen peroxide (H₂O₂), a reactive oxygen species (ROS), directly in cell culture or in defined enzyme reactions. A derivatised luciferin substrate is incubated with sample and reacts directly with H₂O₂ to generate a luciferin precursor. The ROS-Glo™ detection solution converts the precursor to luciferin and provides Ultra-Glo™ Recombinant Luciferase to produce light signal that is proportional to the level of H₂O₂ present in the sample.

Supplier: Promega

CellTiter 96® Non-radioactive Cell Proliferation Assay is a collection of qualified reagents that provide a convenient method of determining viable cell number. The CellTiter 96® Assay is a modification of the MTT assay method described by Mosmann and incorporates several improvements to the method that address previous technical problems, including serum protein precipitation caused by adding organic solvent, interference by phenol red, incomplete solubilisation of the formazan crystals resulting in lower sensitivity, and stability of the coloured product.

Supplier: Promega

The Luciferase Assay System is an extremely sensitive and rapid reagent for quantitation of firefly luciferase. Linear results are seen over at least eight orders of magnitude of enzyme concentration, and less than 10 to 20 moles of luciferase can be measured under optimal conditions. Generally, 100-fold greater sensitivity can be achieved over the chloramphenicol acetyltransferase (CAT) assay.

Supplier: Promega

The NAD/NADH-Glo™ assays are bioluminescent, homogeneous, single-reagent-addition methods to rapidly detect NAD+ and NADH levels in cells and enzymatic reactions. Usual methods to detect nicotinamide adenine dinucleotides lack sensitivity and reproducibility and require sample manipulation and large numbers of cells for cell-based applications. The sensitivity and robustness of the assay chemistry allows fewer cells to be used to detect individual nucleotides directly in multiwell plates or analysis of enzymes with low activity or with low Km values.

Supplier: Promega

The Glycogen-Glo™ Assay is a fast and sensitive method for detecting glycogen from various biological samples, such as cultured cells and tissue homogenates.

Supplier: Promega

The GSH/GSSG-Glo™ Assay is a luminescence-based system for the detection and quantification of total glutathione (GSH +GSSG), GSSG and GSH/GSSG ratios in cultured cells. A change in GSH levels is important in the assessment of toxicological responses and is an indicator of oxidative stress, potentially leading to apoptosis or cell death. The assay provides a simple, rapid multiwell-plate format where stable luminescent signals are correlated with either the total GSH or the GSSG concentration of a sample directly in culture wells.

Supplier: Promega

The MultiTox-Fluor Multiplex Cytotoxicity Assay is a single-reagent-addition, homogeneous, fluorescent assay measures cell viability and cytotoxicity by detecting two distinct protease activities. The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant peptide substrate (GF-AFC Substrate). A cell-impermeant, fluorogenic peptide substrate (bis-AAF-R110 substrate) is used to measure dead-cell protease activity that has been released from cells that have lost membrane integrity.

Supplier: Promega

CaspACE™ FITC-VAD-FMK in situ Marker is a fluorescent analogue of the pan caspase inhibitor Z-VAD-FMK carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone). The fluorescein isothiocyanate (FITC) group has been substituted for the carbobenzoxy (Z) N-terminal blocking group to create the fluorescent apoptosis marker. This structure allows delivery of the inhibitor into the cell where it irreversibly binds to activated caspases. The FITC label allows for a single-reagent addition to assay for caspase activity in situ.

Supplier: Promega

High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells is commonly performed by batch processing of 96- and 384-well plates. Steady-Glo® Luciferase Assay System is designed for this purpose of providing long-lived luminescence when added to cultured cells. The homogeneous assay provides signal half-lives of over 5 hours in commonly used cell culture media, and does not require sample preprocessing. Throughput rates of several thousand samples per hour may be achieved with high reproducibility under standard laboratory conditions.

Supplier: Promega

SignaTECT® Calcium/Calmodulin-Dependent Protein Kinase (CaM KII) Assay provides an improved method to quantitate CaM KII protein
kinase activity, both in purified enzyme preparations and in cell or tissue extracts. The method is based on capture of phosphorylated labeled substrates on the SAM2® Biotin capture membrane, which offers low background and high signal-to-noise ratios, even for complex samples such as crude cell extracts, while retaining the high substrate capacity necessary to maintain optimum reaction kinetics.

Supplier: HIMEDIA

Cellular assay, EZAssay™ TBARS estimation kit for lipid peroxidation

Supplier: STEMCELL TECHNOLOGIES

Sensitive fluorescent dye that detects superoxide production in mitochondria - 500 tests.

Supplier: STEMCELL TECHNOLOGIES

ALDEFLUOR™ kit for the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH - 1 kit.

Supplier: STEMCELL TECHNOLOGIES

Staining kit for human CFU-Mk assays - 1 kit.

Supplier: STEMCELL TECHNOLOGIES

Apoptosis staining and detection kit - 100 tests.

Supplier: STEMCELL TECHNOLOGIES

For the detection of CD34+ and ALDHbr cells in human cord blood - 6×10⁷ cells.

Supplier: STEMCELL TECHNOLOGIES

Fluorescent hydrophobic dye that selectively accumulates in mitochondria - 500 tests.

Supplier: STEMCELL TECHNOLOGIES

For detection of caspase-3/7 activity in cell lysates prepared from mammalian cells undergoing apoptosis - 200 tests.

Supplier: STEMCELL TECHNOLOGIES

Apoptosis staining and detection kit - 100 tests.

Supplier: STEMCELL TECHNOLOGIES

Apoptosis staining and detection kit - 100 tests.

Supplier: STEMCELL TECHNOLOGIES

Complete kit for optimisation of genome editing in human cells.

Supplier: STEMCELL TECHNOLOGIES

Fluorescent hydrophobic dye that selectively accumulates in mitochondria - 500 tests.