You searched for: Cellular Assays

Supplier: Promega

CellTiter-Fluor™ Cell Viability Assay is a a non-lytic, single-reagent-addition fluorescence assay that measures the relative number of viable cells in a population based on measurement of conserved and constitutive protease activity within live cells.

Supplier: Promega

NADPH regeneration system is an enzyme substrate combination that reduces NADP+ to NADPH.

Supplier: Promega

The P450-Glo CYP1B1 assays provide a homogeneous, luminescent method for measuring cytochrome CYP1B1 activity. These assays can be used to selectively measure CYP1A1 activity in hepatocytes where cross-reacting CYP1B1 and CYP3A7 are absent.

Supplier: Promega

Renilla Luciferase assay system is designed to provide a fast, sensitive method of detecting luciferase from sea pansy (Renilla reniformis). The system is a convenient alternative to firefly (Photinus pyralis) reporter systems and is designed to yield reliable, linear results for a concentration range over 7 orders of magnitude.

Supplier: Promega

The Renilla-Glo® Luciferase Assay System is a single-addition reagent that generates a glow-type signal with Renilla luciferase. It has the capacity to lyse cells, reduce the auto luminescence of the coelenterazine substrate and produce a stable signal (half-life greater than 60 minutes at 22 °C).

Supplier: Promega

The P450-Glo CYP3A4 with luciferin-PPXE assays provide a homogeneous, luminescent method for measuring cytochrome CYP3A4 activity in hepatocytes.

Supplier: Promega

The Apo-ONE® Homogeneous Caspase-3/7 Assay provides the necessary reagents for fast and sensitive measurement of active caspase-3 and -7 in a homogeneous format.

Supplier: Promega

The Caspase-Glo® 8 Assay is a homogeneous luminescent assay that measures caspase-8 activity. The assay provides a proluminescent caspase-8 substrate in a buffer system optimised for caspase-8 activity, luciferase activity and cell lysis. The addition of a single Caspase-Glo® 8 reagent results in cell lysis, followed by caspase cleavage of the substrate and generation of "glow-type" luminescent signal. The signal is proportional to caspase-8 activity.

Supplier: Promega

The P450-Glo CYP1B1 assays provide a homogeneous, luminescent method for measuring cytochrome CYP1B1 activity. These assays can be used to detect a mixture of CYP1A1 and CYP1B1 activities in cell types that express both enzymes.

Supplier: Promega

The Kinase-Glo® Plus Luminescent Kinase Assays are homogeneous non-radioactive methods for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction. Kinase-Glo® Plus Assay is linear to 100 μM ATP.

Supplier: Promega

The CellTiter-Blue® Cell Viability Assay provides a homogeneous, fluorescent method for monitoring cell viability. The assay is based on the ability of living cells to convert a redox dye (resazurin) into a fluorescent end product (resorufin). Nonviable cells rapidly lose metabolic capacity and thus do not generate a fluorescent signal.

Supplier: Promega

The β-Galactosidase Enzyme Assay System with reporter lysis buffer is a convenient method for assaying β-galactosidase activity in lysates prepared from cells transfected with β-galactosidase reporter vectors such as the pSV-β-Galactosidase control vector.

Supplier: Promega

The CytoTox-ONE™ Homogeneous Membrane Integrity Assay is a fluorometric method for estimating the number of non viable cells present in multiwell plates.

Supplier: Promega

The CytoTox-Glo™ Assay is a luminescent cytotoxicity assay that measures the relative number of dead cells in cell populations.

Supplier: Promega

High-throughput quantitation of firefly (Photinus pyralis) luciferase expression in mammalian cells requires highly sensitive reagents that can adapt to continuous-process robotic systems. Bright-Glo™ Luciferase Assay System is designed specifically to meet the needs of continuous-process systems by providing robust, homogeneous assay chemistry that achieves high assay sensitivity and approximately 30-minutes signal half-life without prior sample processing.

Supplier: Promega

The ADCC Reporter Bioassay provides a biologically relevant and specific MOA-based measure of ADCC without the complex workflow and variability inherent in primary cell-based assays.

Supplier: Promega

EnduRen™ Live Cell Substrate is useful to detect live cell kinetic measurements, streamline assay development and for multiplexing with other lytic assays.

Supplier: Promega

The cAMP-Glo™ Assay is designed to monitor cAMP production in response to the effects of test compounds on G protein-coupled receptors (GPCR). GPCRs that couple with adenylate cyclase will increase or decrease intracellular cAMP. The assay is based on the principle that cyclic AMP (cAMP) stimulates protein kinase A (PKA) holoenzyme activity, decreasing available ATP and leading to decreased light production in a coupled luciferase reaction.

Supplier: Promega

The Dual-Glo® Luciferase Assay System is a homogeneous reagent system that enables fast and simple quantisation of a stable luminescent signal from two reporter genes in a single sample. The system is optimised for batch processing both 96- and 384-well plates and is compatible with a wide variety of mammalian cell culture media.

Supplier: Promega

GSH-Glo™ Assay is a luminescent-based assay for the detection and quantification of glutathione (GSH) in cells or in various biological samples. The assay is based on the conversion of a luciferin derivative into luciferin in the presence of GSH.

Supplier: Promega

The RealTime-Glo™ MT Cell Viability Assay is a non lytic, homogeneous, bioluminescent method to measure cell viability in real time using a simple, plate-based method. RealTime-Glo™ MT Cell Viability Assay determines the number of viable cells in culture by measuring the reducing potential of cells and thus metabolism (MT).

Supplier: Promega

The P450-Glo CYP3A4 with luciferin-IPA assays provide a homogeneous, luminescent method for measuring cytochrome CYP3A4 activity. These assays can be used to selectively measure CYP3A activity in hepatocytes.

Supplier: Promega

CellTiter-Glo® One Solution Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, which indicates the presence of metabolically active cells.

Supplier: Promega

The Cell-Based Proteasome-Glo™ Assays are homogeneous, luminescent assays that individually measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome complex in cultured cells. These assays can also be used to screen inhibitor libraries and monitor proteasome-regulated protein degradation in cells.

Supplier: Promega

The P450-Glo CYP2C9 assays provide a homogeneous, luminescent method for measuring cytochrome CYP2C9 activity in human hepatocytes and other human cell types.

Supplier: Promega

The CellTiter-Glo 2, 0 assay provides a homogeneous method of determining the number of viable cells in culture by quantitating the amount of ATP present, which indicates the presence of metabolically active cells.

Supplier: Promega

The Caspase-Glo® 3/7 assay is a homogeneous luminescent assay that measures caspase-3/7 activities. The assay provides a proluminescent caspase-3/7 DEVD-aminoluciferin substrate and a proprietary thermostable luciferase in a reagent optimised for caspase-3/7 activity, luciferase activity and cell lysis. This liberates free aminoluciferin, which is consumed by the luciferase, generating a 'glow-type' luminescent signal. The signal is proportional to caspase-3/7 activity. It is designed for use with multiwell plate formats using either purified enzyme or cells in culture.

Supplier: Promega

The P450-Glo CYP2C8 assays provide a homogeneous, luminescent method for measuring cytochrome CYP2C8 activity.

Supplier: Promega

The PepTag® Non-Radioactive Protein Kinase Assay Systems use brightly coloured fluorescent peptide substrates that are highly specific for PKC (PepTag® C1 Peptide-PLSRTLSVAAK) and PKA (PepTag® A1 Peptide-LRRASLG). Phosphorylation of the peptide alters the net charge from +1 to –1, allowing phosphorylated and non phosphorylated versions of the substrate to be rapidly separated on an agarose gel at neutral pH.

Supplier: Promega

The BacTiter-Glo™ Microbial Cell Viability Assay is used for determining the number of viable microbial cells in culture based on quantitation of the ATP present. This homogeneous assay involves adding a single reagent, BacTiter-Glo™ Reagent, directly to bacterial cells cultured in medium and measuring luminescence.