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You searched for: Cellular Assays

Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

Calpain-Glo™ Protease Assay

Calpain-Glo™ Protease Assay

Supplier: Promega

The Calpain-Glo™ Protease Assay is a homogeneous, luminescent assay that measures calpain 1 (µ) and 2 (m) activities.

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Mitochondrial toxicity assays, Mitochondrial ToxGlo™

Mitochondrial toxicity assays, Mitochondrial ToxGlo™

Supplier: Promega

The Mitochondrial ToxGlo™ Assay is a cell-based assay method that employs a sequential addition, multiplexed assay chemistry for predicting potential mitochondrial dysfunction as a result of xenobiotic exposure.

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Cytotoxicity assays CytoTox-Glo™

Cytotoxicity assays CytoTox-Glo™

Supplier: Promega

The CytoTox-Glo™ Assay is a luminescent cytotoxicity assay that measures the relative number of dead cells in cell populations.

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CYP1A2 assay and screening systems, P450-Glo™

CYP1A2 assay and screening systems, P450-Glo™

Supplier: Promega

The P450-Glo CYP1A2 assays provide a homogeneous, luminescent method for measuring cytochrome CYP1A2 activity.

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Beta Galactosidase (β-Gal) assay system, Beta-Glo®

Beta Galactosidase (β-Gal) assay system, Beta-Glo®

Supplier: Promega

The Beta-Glo® Assay System is a homogeneous method of quantitating β-galactosidase expression in mammalian cells.

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CellTiter 96® AQueous MTS Reagent Powder

CellTiter 96® AQueous MTS Reagent Powder

Supplier: Promega

CellTiter 96® AQueous MTS Reagent Powder is a novel tetrazolium compound used in colorimetric assays to determine the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays.

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Cell viability, apoptosis and cytotoxicity, ApoTox-Glo™ Triplex Assay

Cell viability, apoptosis and cytotoxicity, ApoTox-Glo™ Triplex Assay

Supplier: Promega

The ApoTox-Glo™ Triplex Assay combines three assay chemistries to easily assess viability, cytotoxicity and apoptosis events in the same cell-based assay well.

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Luminescent kinase assay, Kinase-Glo®

Luminescent kinase assay, Kinase-Glo®

Supplier: Promega

The Kinase-Glo® Luminescent Kinase Assays are homogeneous non-radioactive methods for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction. Kinase-Glo® Assay is linear to 10 μm ATP.

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Luciferase assay system, ONE-Glo™

Luciferase assay system, ONE-Glo™

Supplier: Promega

The ONE-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous assay for detection of firefly luciferase reporter gene expression in mammalian cells in 96- and 384-well plates.

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Cell signaling assays, ProFluor® Ser/Thr PPase Assay

Supplier: Promega

ProFluor® Ser/Thr PPase Assay measures purified serine/threonine protein phosphatase activity in a multiwell plate format and involves 'add-mix-read' steps only. The assay works with protein phosphatase 1 (PP1), PP2A, PP2B and PP2C.

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MultiTox-Glo multiplex cytotoxicity assay

MultiTox-Glo multiplex cytotoxicity assay

Supplier: Promega

The MultiTox-Glo Multiplex Cytotoxicity Assay is a sequential-reagent-addition fluorescent and luminescent assay that measures the relative number of live and dead cells in cell populations.

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NADPH regeneration system

NADPH regeneration system

Supplier: Promega

NADPH regeneration system is an enzyme substrate combination that reduces NADP+ to NADPH.

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Renilla luciferase assay system

Renilla luciferase assay system

Supplier: Promega

Renilla Luciferase assay system is designed to provide a fast, sensitive method of detecting luciferase from sea pansy (Renilla reniformis). The system is a convenient alternative to firefly (Photinus pyralis) reporter systems and is designed to yield reliable, linear results for a concentration range over 7 orders of magnitude.

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CellTiter-Glo® 2,0 Cell Viability Assay Kits

CellTiter-Glo® 2,0 Cell Viability Assay Kits

Supplier: Promega

The CellTiter-Glo 2, 0 assay provides a homogeneous method of determining the number of viable cells in culture by quantitating the amount of ATP present, which indicates the presence of metabolically active cells.

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Apoptosis assays, Apo-ONE™ Homogeneous Caspase-3/7

Apoptosis assays, Apo-ONE™ Homogeneous Caspase-3/7

Supplier: Promega

The Apo-ONE® Homogeneous Caspase-3/7 Assay provides the necessary reagents for fast and sensitive measurement of active caspase-3 and -7 in a homogeneous format.

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cAMP assay, GloSensor™

cAMP assay, GloSensor™

Supplier: Promega

The GloSensor™ cAMP assay provides an extremely sensitive and easy to use format for the interrogation of overexpressed or endogenous GPCRs that signal via changes in the intracellular concentration of cAMP.

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Luciferase assay system, Bio-Glo™

Luciferase assay system, Bio-Glo™

Supplier: Promega

The Bio-Glo™ Luciferase Assay System provides a highly sensitive, robust, homogeneous reagent for the detection of firefly luciferase reporter gene expression in the ADCC Reporter Bioassay.

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Griess reagent system

Griess reagent system

Supplier: Promega

The Griess Reagent System measures nitrite (NO²⁻), which is one of two primary stable and non volatile breakdown products of nitric oxide (NO).

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HDAC-Glo™ I/II assays and screening systems

HDAC-Glo™ I/II assays and screening systems

Supplier: Promega

The HDAC-Glo I/II Assays and Screening Systems are single-reagent-addition, homogeneous, luminescent assays that measure the relative activity of histone deacetylase (HDAC) class I and II enzymes from cells, extracts, or purified enzyme sources.

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CYP2C8 assay systems, P450-Glo™

CYP2C8 assay systems, P450-Glo™

Supplier: Promega

The P450-Glo CYP2C8 assays provide a homogeneous, luminescent method for measuring cytochrome CYP2C8 activity.

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Proteasome-Glo™ assays

Proteasome-Glo™ assays

Supplier: Promega

The Proteasome-Glo™ 3-Substrate System consists of three homogeneous bioluminescent assays that individually measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome in a purified enzyme-based format. Each of these three assays is also available separately.

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Cell viability assays, CytoTox-ONE™ Homogeneous membrane integrity assay

Cell viability assays, CytoTox-ONE™ Homogeneous membrane integrity assay

Supplier: Promega

The CytoTox-ONE™ Homogeneous Membrane Integrity Assay is a fluorometric method for estimating the number of non viable cells present in multiwell plates.

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Colorimetric TUNEL system, DeadEnd™

Supplier: Promega

The DeadEnd™ Colorimetric TUNEL system is a modified TUNEL (TdT-mediated dUTP Nick-End Labeling) assay used to detect the apoptotic cells in situ at the single-cell level.

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Live cell substrate, EnduRen™

Live cell substrate, EnduRen™

Supplier: Promega

EnduRen™ Live Cell Substrate is useful to detect live cell kinetic measurements, streamline assay development and for multiplexing with other lytic assays.

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Cell proliferation assays, CellTiter 96® AQueous One Solution

Cell proliferation assays, CellTiter 96® AQueous One Solution

Supplier: Promega

The CellTiter 96® AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays.

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Fluorometric TUNEL system, DeadEnd™

Fluorometric TUNEL system, DeadEnd™

Supplier: Promega

The DeadEnd™ Fluorometric TUNEL system is a classic TUNEL (TdT-mediated dUTP Nick-End Labeling) assay designed for the specific detection and quantitation of apoptotic cells within a cultured cells or FFPE tissue sections.

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CYP2C19 assay and screening systems, P450-Glo™

CYP2C19 assay and screening systems, P450-Glo™

Supplier: Promega

The P450-Glo CYP2C19 assays provide a homogeneous, luminescent method for measuring cytochrome CYP2C19 activity.

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CYP2D6 assay and screening systems, P450-Glo™

CYP2D6 assay and screening systems, P450-Glo™

Supplier: Promega

The P450-Glo CYP2D6 assays provide a homogeneous, luminescent method for measuring cytochrome CYP2D6 activity.

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Kinase assay, ADP-Glo™

Kinase assay, ADP-Glo™

Supplier: Promega

The ADP-Glo™ kinase assay measures ADP formed from a kinase reaction. The ADP is converted into ATP, which is used to generate light in a luciferase reaction. The luminescence generated correlates with kinase activity.

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Dual-Luciferase® reporter (DLR™) assay system

Dual-Luciferase® reporter (DLR™) assay system

Supplier: Promega

The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample.

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