You searched for: Proteins and Peptides

Supplier: ENZO LIFE SCIENCES

Produced in E.coli. Active Matrix Metalloproteinase-14 (MMP-14, Membrane-Type Matrix Metalloproteinase 1, MT1-MMP) catalytic domain from human cDNA.

Supplier: ENZO LIFE SCIENCES

Integrin αvβ3 antagonist. Inhibits cell adhesion events mediated by αvβ3. Inhibits angiogenesis. Induces rapid regression of histologically distinct human tumors. Induces apoptosis of angiogenic blood vessels.

Supplier: ENZO LIFE SCIENCES

Fluorogenic substrate cleaved by plasma kallikrein and kallikrein 8, but not plasmin; by papain; and by most cathepsins (cathepsins B, C/DPP-I, F, K/O2, L, L2/V, O, S, and X/Z). Ex.: 380 nm, Em.: 460 nm, although the following Ex/Em can also be used: 355,375/440,450.

Supplier: OriGene

This gene encodes an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. The encoded protein binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). Alternative splicing results in multiple transcript variants.

Supplier: ENZO LIFE SCIENCES

Highly-quenched, ultra-bright fluorogenic substrate for most MMPs. 6'-TAMRA fluorescence is thoroughly quenched by the TQ3 group until cleavage by MMPs separates the two moieties. The OMNIMMP® RED substrate offers key advantages over other MMP substrates.

Supplier: ENZO LIFE SCIENCES

The HIGHDEF® DAB Chromogen/Substrate Set can be used in conjunction with peroxidase-based immunostaining systems. Peroxidase from the antibody detection system reacts with hydrogen peroxide substrate to degrade it, which then reacts with DAB, precipitating it at positive sites and yielding a dark brown color.

Supplier: Shenandoah Biotechnology

B cell-attracting chemokine 1 (BCA-1), also known as CXCL13, is expressed at high levels in lymphoid tissues, such as the spleen, lymph nodes, and Peyer's patches.  BCA-1 activates signaling through the receptor Burkitt lymphoma receptor 1 (BLR1) to chemoattract B cells.

Supplier: ProSci Inc.

Protein G is an immunoglobulin-binding protein expressed in group C and G Streptococcal bacteria much like Protein A but with differing specificities. It is a 65-kDa (G148 protein G) and a 58 kDa (C40 protein G) cell surface protein that has found application in purifying antibodies through its binding to the Fc region. The native molecule also binds albumin, however, because serum albumin is a major contaminant of antibody sources, the albumin binding site has been removed from this recombinant form of Protein G.

Supplier: ProSci Inc.

ADAM DEC1 protein is expressed highly in the small intestine and appendix, moderately in lymph node, mucosal lining of the colon, thymus, spleen and very weakly in the bone marrow. ADAM DEC1 is induced during DC maturation and up-regulated in response to T-cell signals. It may play an important role in the control of the immune response and during pregnancy.

Supplier: SELENOZYME

The TrxR enzyme in C. elegans is the single selenoprotein in this organism, exerting a wide range of functions in redox control and for antioxidant systems. It is also together with glutathione reductase essential for the molting process.
We provide the main isoform of the C. elegans TrxR enzyme with more than 90% Sec contents and more than 95% purity in aliquots at amounts from 5 to 50 units.

Supplier: ENZO LIFE SCIENCES

This BSA Solution (10%) is specially designed to create the Blocking Buffer and Assay Buffer in ImmunoSet products. Each bottle is sufficient for use with 5×96 wells microtiter plates if following the instructions in our ImmunoSet protocol. The BSA Solution may be used for any other assay development needs. Additional assay development reagents are available as companion products to our ImmunoSet products.

Supplier: Biowest

Bovine serum albumin (BSA) is commonly used in cell culture protocols, particularly where protein supplementation is necessary and the other components of serum are unwanted. In cell culture, its main role is as a carrier of small molecules. Because of its negative charge, BSA binds water, salts, fatty acids, vitamins and hormones, then carries these bound components between tissues and cells. The binding capacity also makes BSA an effective scavenger to remove toxic substances, including pyrogens, from the medium.

Supplier: SELENOZYME

The human DIO2 selenocprotein is encoded by the DIO2 gene and catalyzes the conversion of prohormone thyroxine (3,5,3',5'-tetraiodothyronine, T4) to the bioactive thyroid hormone (3,5,3'-triiodothyronine, T3) by outer ring 5'-deiodination.
We provide the human DIO2 enzyme with approx. 20% Sec contents, mixed with inactive forms containing Sec-to-Lys and Sec-to-Gln substitutions, at more than 95% purity in aliquots at amounts from 50 to 500 µg.

Supplier: ProSci Inc.

Bcl-2-like protein 2 (BCL2L2) belongs to the Bcl-2 family. BCL2L2 is highly expressed in thebrain, spinal cord, testis, pancreas, heart, spleen, and mammary glands. BCL2L2 is a peripheral membrane protein containing three motifs, BH1, BH2 and BH4. The BH4 motif appears to be involved in the anti-apoptotic function. The BH1 and BH2 motifs form a hydrophobic groove which acts as a docking site for the BH3 domain of some pro-apoptotic proteins. BCL2L2 promotes cell survival and blocks dexamethasone-induced apoptosis. Furthermore, BCL2L2 mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX.

Supplier: Shenandoah Biotechnology

Cytokines for polarizing T cells into T regulatory cells. T regulatory cells (Tregs) are a subset of T cells that suppress immune function. They are characterized by the expression of CD4, CD25 and Foxp3 and are known to produce immunosuppressive cytokines TGF-β 1 and IL-10. Tregs can be cultured from naive CD4+ T cells by culturing them in the presence of IL-2 and TGF-β 1 with CD3 and CD28 stimulation. In some cases cells are also cultured with neutralizing antibodies to IL-4, IFNγ and IL-12 and/or retinoic acid.

Supplier: SELENOZYME

The TGR selenoprotein of Schistosoma mansoni is the only major reductive cytosolic flavoprotein of this parasite, taking over the combined role of TrxR1 and glutathione reductase, thereby exerting a wide range of functions in redox control and antioxidant systems and being a promising drug target for treatment of schistosomiasis.
We provide the main the Schistosoma mansoni TGR enzyme with more than 90% Sec contents and more than 95% purity in aliquots at amounts from 5 to 50 units.

Supplier: Biosensis

Mouse NGF (2.5S) was isolated from mouse submaxillary glands by method of Mobley et al (1976) and is a form of beta-NGF that has identical biological properties. NGF is known to regulate the survival and development of certain sympathetic and sensory neurons. It is a dimer with 2 identical polypeptide chains and dimeric molecular weight of approximately 26,500 Da. Isolation and purification of NGF from mouse submaxillary glands yields preparations of NGF (2.5S) with identical biological activity but with cleavages at the amino terminus (with the loss of 8 amino acids) and/or at the carboxy-terminus (with the loss of arginine). These preparations are named 2.5 NGF.

Supplier: ENZO LIFE SCIENCES

Quenched fluorogenic substrate for cathepsin D (kcat/Km=1,09×10 M-1s-1) and cathepsin E (kcat/Km=1,56×10 M-1s-1). Mca fluorescence is quenched by the Dnp group until cleavage separates them. Activity of the two enzymes on this substrate can be distinguished by the use of Ascaris pepsin inhibitor, which inhibits cathepsin E, but not cathepsin D. This substrate is also cleaved by MMP-2, MMP-3, MMP-9, but not MMP-1 nor MMP-13. This substrate is useful for inhibitor screening and kinetic analysis. Ex.: 320-340 nm, Em.: 393-420 nm. Mca-Pro-Leu-OH (BML-P127) can be used to calibrate for Mca fluorescence.

Supplier: ENZO LIFE SCIENCES

Microcystins are a group of cyclic heptapeptide hepatotoxins produced by a number of cyanobacterial genera. The most notable of which, and namesake, is the widespread genus Microcystis. Structurally, all microcystins consist of the generalized structure cyclo(-D-Ala1-X2-D-MeAsp3-Y4-Adda5-D-Glu6-Mdha7-). X and Y are variable L-amino acids, D-MeAsp is D-erythro-β-methylaspartic acid and Mdha is N-methyldehydroalanine. Adda is the cyanobacteria unique C20 β-amino acid 3-amino-9-methoxy-2,6,8-trimethyl-10-phenyl-deca-4,6-dienoic acid. Substitutions of the variable L-amino acids at positions 2 and 4 give rise to at least 21 known primary microcystin analogs and alterations in the other constituent amino acids result in more than 90 reported mycrocystins to date.

Supplier: ENZO LIFE SCIENCES

Fluor de Lys® Substrate is a highly sensitive and convenient alternative to radiolabeled, acetylated histones or peptide/HPLC methods for the assay of histone deacetylases. The Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with a sample containing HDAC activity (nuclear or cellular extract, purified enzyme, bead-bound immunocomplex, etc.). Deacetylation of the substrate sensitizes the substrate so that, in a second step, treatment with the Fluor de Lys® Developer produces a fluorophore. Histone deacetylases from all known classes have been successfully assayed with the Fluor de Lys® Substrate. These include human HDACs 1, 2, 3 and 8 (class I), human HDACs 4 and 9 (class II) and the sirtuins yeast Sir2, human SIRT1 and human SIRT2 (class III; reaction must include the sirtuin substrate NAD+). The substrate is cell-permeable and serves as the basis for the HDAC Cellular Activity Assay Kit (Prod. No. BML-AK503). NOTE: Must be used in conjunction with Fluor de Lys® Developer (Prod. No. BML-KI105). Sufficient for 100-1000 assays of HDACs or sirtuins (50 µl reaction volume, 50-500 µM (substrate).

Supplier: Shenandoah Biotechnology

Cytokines for expansion and cell culture of human hematopoietic stem cells. Hematopoietic stem cells are self renewing pluripotent cells often characterised by the expression of CD34. Studies have shown that decreased expression of CD34 tends to correlate with decreased self renewal properties and pluripotent potential. Culturing CD34+ cells in a cytokine mixture of TPO, SCF, and FLT-3 Ligand for short time periods allows CD34+ cells to proliferate without decreasing CD34 expression, thus retaining their stem cell properties. The combination of these cytokines allows for the ex vivo expansion of the most immature hematopoietic stem cells in cord blood or bone marrow preparations, but can be supplemented with other cytokines to promote specific lineages. For example, GM-CSF and/or G-CSF can be added to promote monocytes and granulocytes while EPO can be added to promote erthroid cells.

Supplier: ProSci Inc.

Neutrophil gelatinase-associated lipocalin(LCN2) is a secreted protein and belongs to the calycin superfamily. This protein is released from injured tubular cells after various damaging stimuli, is already known by nephrologists as one of the most promising biomarkers of incoming Acute Kidney Injury (AKI). Recent evidence also suggests its role as a biomarker in a variety of other renal and non-renal conditions. Moreover, recent studies seem to suggest a potential involvement of this factor also in the genesis and progression of chronic kidney diseases. NGAL is the first known mammalian protein which specifically binds organic molecules called siderophores, which are high-affinity iron chelators. NGAL, first known as an antibacterial factor of natural immunity, and an acute phase protein, is currently one of the most interesting and enigmatic proteins involved in the process of tumor development. acting as an intracellular iron carrier and protecting MMP9 from proteolytic degradation, NGAL has a clear pro-tumoral effect, as has already been observed in different tumors (e.g. breast, stomach, oesophagus, brain) in humans. In thyroid carcinomas, NGAL is strongly induced by NF-kB, an important factor involved both in tumor growth and in the link between chronic inflammation and neoplastic development. Thus, Lipocalin-2 (LCN2/NGAL) has been implicated in a variety of processes including cell differentiation, proliferation, survival and morphogenesis.

Supplier: ANTIBODIES.COM

Recombinant Human Peptide YY/PYY (from HEK293 cells)

Supplier: ENZO LIFE SCIENCES

AMPK substrate

Supplier: Apollo Scientific

Recombinant Protein G

Supplier: ENZO LIFE SCIENCES

Mast cell degranulating peptide.

Supplier: ENZO LIFE SCIENCES

Mast cell degranulating peptide.

Supplier: Abcam

Protein A (Gold)

Supplier: Apollo Scientific

Avidin

Supplier: Apollo Scientific

Buserelin