Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
Wheat Germ Agglutinin, XFD647 Labelled *XFD647 Same Structure to Alexa Fluor® 647*
Supplier: AAT BIOQUEST
XFD647, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 647, a trademark of ThermoFisher.
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Buccutite™ Rapid APC-iFluor® 700 Tandem Antibody Labelling Kits (2 Labellings)
Supplier: AAT BIOQUEST
APC-iFluor® 700 Tandem is an excellent replacement for APC-Alexa Fluor® 700 Tandem since they have almost identical spectra.
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HIS Lite™ iFluor® 647 Tris NTA-Ni Complex
Supplier: AAT BIOQUEST
iFluor 647-Tris NTA compound is used as a sensitive fluorescent probe for detecting polyhistidine-labelled proteins in cells, solution and solid surfaces.
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Buccutite™ Rapid APC-iFluor® 700 Tandem Antibody Labelling Kit
Supplier: AAT BIOQUEST
Production scale optimised for labelling 1 mg antibody per reaction. Buccutite™ rapid APC-iFluor® 700 tandem antibody labelling kits, designed for large-scale production, provide a streamlined approach for labelling antibodies with PE, APC, PerCP and iFluor® tandem dyes.
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MitoROS™ 580 Kit for Detecting Reactive Oxygen Species (ROS) in Mitochondria
Supplier: AAT BIOQUEST
Oxidation of MitoROS™ 580 by superoxide generates red fluorescence.
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Buccutite™ Rapid APC Antibody Labelling Kits (2 Labellings)
Supplier: AAT BIOQUEST
APC is an red fluorescent protein which has an excitation wavelength of 651 nm and an emission wavelength of 662 nm.
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ReadiLink™ Rapid mFluor™ Violet 610 Antibody Labelling Kit
Supplier: AAT BIOQUEST
Microscale optimised for labeling 50 µg antibody per reaction. ReadiLink™ Rapid mFluor™ Violet 610 antibody labelling kits provide a quick and convenient method to label antibodies, proteins (<10 kDa), or other amine-containing biomolecules.
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Buccutite™ Rapid PE Antibody Labelling Kits (2 Labellings)
Supplier: AAT BIOQUEST
R-Phycoerythrin (PE) is an orange fluorescent protein which has an excitation wavelength of 565 nm and an emission wavelength of 575 nm.
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ReadiLink™ iFluor® 647 Nick Translation dsDNA Labeling Kit
Supplier: AAT BIOQUEST
It is compatible with a wide variety of sample materials, including bacterial artificial chromosome (BAC) DNA, human genomic DNA, purified PCR products, supercoiled and linearized plasmid DNA.
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ReadiLink™ Rapid iFluor® 647 Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT BIOQUEST
AAT Bioquest's iFluor® dyes are optimised for labelling proteins, in particular, antibodies.
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ReadiLink™ Rapid XFD488 Antibody Labelling Kit 'XFD488 Same Structure to Alexa Fluor™ 488' (2×50 µg Labellings)
Supplier: AAT BIOQUEST
XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher).
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ReadiLink™ Rapid Cy7 Antibody Labelling Kit
Supplier: AAT BIOQUEST
ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with Cy7.
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ReadiLink™ Rapid Cy3 Antibody Labelling Kit
Supplier: AAT BIOQUEST
ReadiLink™ Rapid antibody labelling kits, designed for production scale, provide a convenient and efficient method for labelling large volumes of antibodies with Cy3.
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Fluorescent dye removal columns, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions.
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HIS Lite™ iFluor® 568 Tris NTA Chelator
Supplier: AAT BIOQUEST
iFluor® 568 Tris-NTA Chelator is used as a sensitive fluorescent probe for detecting polyhistidine-labelled proteins in combination with the addition of a heavy metal ion (such as Ni²⁺) in cells, solution and solid surfaces.
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Pierce™, EZ-Link™ Sulfhydryl Reactive Biotinylation Reagents
Supplier: Thermo Fisher Scientific
Sulfhydryl groups are the second most common target for modification in biological studies. They are found as exposed cysteines in protein and peptides. Sulfhydryl group biotinylation provides an advantage in some applications; for example, targeting a sulfhydryl group can help preserve the biological activity of an enzyme when amines are found at the active site.
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ReadiLink™ DIG (Digoxigenin) Oligo and ssDNA Labeling Kit
Supplier: AAT BIOQUEST
The kit is optimised for efficient labelling and contains all the essential reagents required for efficient labelling of ssDNA or oligos.
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ReadiLink™Rapid mFluor™ Antibody Labeling Kit "Microscale Optimized for Labeling 50 µg Antibody Per Reaction" (2 Labelings)
Supplier: AAT BIOQUEST
mFluor™ dyes are developed for flow cytometry-focused applications.
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HIS Lite™ Cy3 Bis NTA Chelator
Supplier: AAT BIOQUEST
Cy3 Bis NTA-Ni Chelator can be applied either to an SDS-PAGE gel or Western blot membrane for fluorescence imaging or used in living cells in combination with the addition of a heavy metal ion such as Ni²⁺.
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Helixyte™ iFluor® 594 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 µg DNA/RNA*
Supplier: AAT BIOQUEST
Helixyte™ iFluor® 594 Nucleic acid labelling dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.
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Helixyte™ iFluor® 350 Nucleic Acid Labelling Dye *Optimised for Labelling 2×100 μg DNA/RNA*
Supplier: AAT BIOQUEST
Helixyte™ iFluor® 350 Nucleic Acid labelling Dye is a key member of our enabling Helixyte™ nucleic acid labelling and conjugation technology.
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ReadiLink™ xtra Rapid XFD488 Antibody Labelling Kit *BSA-Compatible, XFD488 Same Structure to Alexa Fluor™ 488* (2 Labelings)
Supplier: AAT BIOQUEST
XFD488 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 488 (Alexa Fluor® is the trademark of ThermoFisher).
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ReadiLink™ BSA Conjugation Kit (2 Reactions)
Supplier: AAT BIOQUEST
Bovine serum albumin (BSA) is a serum albumin protein that has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry.
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CytoWatch™ Wash-free Fluorescence Cell Imaging Buffer, 10X
Supplier: AAT BIOQUEST
CytoWatch™ Wash-free fluorescence cell imaging buffer *10X* is a ready-to-use buffer optimised for fluorescence cell imaging.
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Pierce™ Deuterium-labelled Cross Linkers
Supplier: Thermo Fisher Scientific
Cross linkers are used for the identification of near-neighbor protein relationships and ligand-receptor interactions.
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Dye labeling kit (FITC), HOOK™
Supplier: G-Biosciences
FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494 nm and 520 nm, respectively, therefore emitting green visible light.
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Wheat Germ Agglutinin, XFD350 Labelled *XFD350 Same Structure to Alexa Fluor® 350*
Supplier: AAT BIOQUEST
XFD350, manufactured by AAT Bioquest, has an identical chemical structure to Alexa Fluor® 350, a trademark of ThermoFisher.
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ReadiLink™ xtra Rapid iFluor® 555 Antibody Labelling Kit *BSA-Compatible* (2 Labellings)
Supplier: AAT BIOQUEST
ReadiLink™ xtra rapid antibody labelling kits require essentially only two simple mixing steps without a column purification needed.
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Streptavidin, hydrazide-activated, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Hydrazide-activated strepavidin conjugate includes recombinant streptavidin in a purified form activated for crosslinking to carbonyl groups in a molecule.
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PEGylated BM (bismaleimido) Crosslinkers, Homobifunctional, Pierce™
Supplier: Thermo Fisher Scientific
Cross linkers are used for the identification of near-neighbour protein relationships and ligand-receptor interactions. Homobifunctional, amine-reactive NHS esters or imidates, and heterobifunctional, amine-reactive, photoactivatable phenyl azides are the most commonly used cross linkers for these applications.