You searched for: Protein Linking and Labeling Systems
Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
CyDye Antibody Labeling Kits
Supplier: Cytiva
CyDye Fluorescent Dyes are supplied as highly water soluble, reactive NHS esters for easy conjugation to biomolecules containing aliphatic amino groups.
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Monofunctional maleimide fluors, CyDye™
Supplier: Cytiva
Cy3 and Cy5 monofunctional maleimides are particularly suitable for the selective labelling of molecules containing free sulfhydryl groups, such as cysteine residues in proteins and peptides and oligonucleotides.
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Amersham CyDye DIGE fluors
Supplier: Cytiva
CyDye™ DIGE fluors are available as minimal and saturation labeling dyes. The minimal dyes are intended for general 2-D application use where sufficient amounts of sample are available. The saturation dyes from the Scarce Sample Labeling Kit are designed to be used for applications where only small amounts of sample are available, for example in Laser Capture Microdissection.
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Translation labelling system, GreenLys in vitro translation labelling system, FluoroTect™
Supplier: Promega
FluoroTect™ GreenLys in vitro Translation Labelling System enables fluorescent labelling and detection of in vitro synthesised proteins, based on a lysine-charged tRNA labelled with the fluorophore BODIPY®-FL at the ε position. Fluorescent lysine residues are incorporated into synthesised proteins during in vitro translation reactions, eliminating the need for radioactivity.
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AP fluorescent substrate kit, AttoPhos®
Supplier: Promega
AttoPhos® AP Fluorescent Substrate System, utilising a highly sensitive fluorescent alkaline phosphatase (AP) substrate, maintains a low fluorescence signal until enzymatically acted upon, yielding detection of AP to as little as 0,1 attomole.
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Ligand building blocks, HaloTag®
Supplier: Promega
Ligand building blocks can carry a variety of functionalities, including fluorescent labels, affinity tags and attachments to a solid phase. The covalent bond forms rapidly under general physiological conditions, is highly specific and essentially irreversible. The HaloTag® Ligand Building Blocks allow researchers to apply the chloroalkane group that HaloTag® protein reacts with to any compound or surface with a compatible chemical group, creating endless possible applications.
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In Vivo Renilla Luciferase substrate, ViviRen™
Supplier: Promega
ViviRen™ in vivo Renilla luciferase substrate is a uniquely engineered coelenterazine-based compound with protected oxidation sites. These modifications are designed to minimise substrate degradation and autoluminescence. ViviRen™ Substrate is reported to demonstrate brighter output compared to the native coelenterazine substrate when used in an in vivo imaging application in a mouse model. High-quality substrate eliminates potential interference in assays due to the presence of endotoxins.
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Fluorescent ligands, HaloTag®
Supplier: Promega
HaloTag® fluorescent ligands form covalent bonds rapidly under general physiological conditions. The bonds are highly specific and essentially irreversible. HaloTag® fluorescent ligands enable the addition of the chloroalkane group, with which HaloTag® protein reacts, to any compound or surface with a compatible chemical group.
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Labeling dye, Atto 488, Sigma-Aldrich®
Supplier: Merck
Atto 488 is a labeling dye with high molecular absorption (90000) and quantum yield (0,80) as well as sufficient stokes shift between excitation and emission maximum. It is optimised for excitation with an argon laser, and is characterised by high photostability.
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Atto 647N-biotin, BioReagent
Supplier: Merck
Designed for high sensitivity applications, including single molecule detection. These labels have rigid structures that do not show any cis-trans-isomerisation and display exceptional intensity with minimal spectral shift on conjugation.
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DNA labeling reagent, Atto 647 NHS Ester
Supplier: Merck
Atto 647 NHS ester is a reagent for the covalent labeling of molecules with Atto 647 flourescent dye.
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LysoBrite™ Lysosome Dye, Red DND-99
Supplier: STEMCELL TECHNOLOGIES
Fluorescent dyes used to label and track lysosomes in live cells - 500 tests.
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GloCell™ Fixable Viability Dye Red 780
Supplier: STEMCELL TECHNOLOGIES
Amine-labelling fluorescent dye for live/dead staining of mammalian cells - 100 tests.
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GloCell™ Fixable Viability Dye Violet 510
Supplier: STEMCELL TECHNOLOGIES
Amine-labelling fluorescent dye for live/dead staining of mammalian cells - 100 tests.
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GloCell™ Fixable Viability Dye Violet 450
Supplier: STEMCELL TECHNOLOGIES
Amine-labelling fluorescent dye for live/dead staining of mammalian cells - 100 tests.
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GloCell™ Fixable Viability Dye Violet 540
Supplier: STEMCELL TECHNOLOGIES
Amine-labelling fluorescent dye for live/dead staining of mammalian cells - 100 tests.
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LysoBrite™ Lysosome Dye, Green
Supplier: STEMCELL TECHNOLOGIES
Fluorescent dyes used to label and track lysosomes in live cells - 500 tests.
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GloCell™ Fixable Viability Dye UV 450
Supplier: STEMCELL TECHNOLOGIES
Amine-labelling fluorescent dye for live/dead staining of mammalian cells - 100 tests.
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GloCell™ Fixable Viability Dye Red 710
Supplier: STEMCELL TECHNOLOGIES
Amine-labelling fluorescent dye for live/dead staining of mammalian cells - 100 tests.
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Annexin V Binding Buffer
Supplier: STEMCELL TECHNOLOGIES
Assay buffer for labeling with Annexin V - 50 ml.
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LysoBrite™ Lysosome Dye, Deep Red
Supplier: STEMCELL TECHNOLOGIES
Fluorescent dyes used to label and track lysosomes in live cells - 500 tests.