Protein Extraction

Supplier: Promega

Rabbit Reticulocyte Lysate System, Nuclease Treated can be used to identify mRNA species, characterise their protein products and investigate transcriptional and translational control.

Supplier: Promega

FastBreak™ cell lysis reagent is provided as a 10X concentrate and contains a proprietary non ionic detergent to facilitate lysis. Recombinant proteins can be directly screened in the cell extract or purified by the addition of the appropriate affinity matrix such as the MagneHis™ protein purification system.

Supplier: Promega

ProteaseMAX™ Surfactant ensures fast and efficient protein digestion with proteases such as trypsin, chymotrypsin and Lys-C.

Supplier: Promega

Trypsin Gold, mass spectrometry grade, has been manufactured to provide maximum specificity. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion. The specificity of the purified trypsin is further improved by TPCK treatment, which inactivates chymotrypsin. The treated trypsin is then purified by affinity chromatography and lyophilised.

Supplier: Promega

Endoproteinase hydrolyses peptide bonds on the N-terminal side of aspartic and cysteic acid residues (Asp and Cys).

Supplier: Promega

rLys-C, MS grade, is a recombinant Lys-C expressed in E. coli. It cleaves at C-terminus of lysines.

Supplier: Promega

Trypsin/Lys-C mix improves peptide digestion over trypsin alone, increasing peptide recovery and resulting in better protein quantitation by using standard digestion conditions.

Supplier: Promega

Arg-C, sequencing grade, is an endopeptidase (clostripain) that cleaves at the C-terminus of arginine residues, including the sites next to proline and it occurs also at proline.

Supplier: Promega

Glu-C sequencing grade serine protease (S. aureus V8) specifically cleaves at the C-terminus of either aspartic or glutamic acid residues.

Supplier: VARIAN

Uniform latex particles are used in a wide range of applications that exploit their physico-chemical properties in sophisticated immunoassay formats, both in medicine and veterinary science research.