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Protein Extraction

Protein Extraction

Protein extraction kits provide a simple, rapid, and reproducible method for preparing total cellular protein extracts from a wide variety of biological samples. The results of these extraction systems generate protein samples that can be applied directly to a variety of applications. High-performance models are designed for efficient recovery of genomic DNA and RNA. These extraction kits are capable of purifying genomic DNA, total RNA, and total protein simultaneously from cultured cells, animal tissues, whole blood, and biological fluids.

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rLys-C, MS grade

rLys-C, MS grade

Supplier: Promega

rLys-C, MS grade, is a recombinant Lys-C expressed in E. coli. It cleaves at C-terminus of lysines.

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Rabbit reticulocyte lysate, untreated

Rabbit reticulocyte lysate, untreated

Supplier: Promega

Rabbit Reticulocyte Lysate, Untreated contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors) but has not been treated with micrococcal nuclease. Untreated Lysate is used primarily for the isolation of these components and as an abundant source of endogenous globin mRNA.

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Rabbit reticulocyte lysate system, Flexi®

Rabbit reticulocyte lysate system, Flexi®

Supplier: Promega

The Flexi® rabbit reticulocyte lysate system allows translation reactions to be optimised for a wide range of parameters, including Mg²⁺ and K⁺ concentrations and the choice to add or omit DTT.

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Rabbit reticulocyte lysate system, nuclease treated

Rabbit reticulocyte lysate system, nuclease treated

Supplier: Promega

Rabbit Reticulocyte Lysate System, Nuclease Treated can be used to identify mRNA species, characterise their protein products and investigate transcriptional and translational control.

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Protease surfactant, trypsin enhancer, ProteaseMAX™

Protease surfactant, trypsin enhancer, ProteaseMAX™

Supplier: Promega

ProteaseMAX™ Surfactant ensures fast and efficient protein digestion with proteases such as trypsin, chymotrypsin and Lys-C.

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Trypsin gold, MS grade

Trypsin gold, MS grade

Supplier: Promega

Trypsin Gold, mass spectrometry grade, has been manufactured to provide maximum specificity. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion. The specificity of the purified trypsin is further improved by TPCK treatment, which inactivates chymotrypsin. The treated trypsin is then purified by affinity chromatography and lyophilised.

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Wheat germ extract

Wheat germ extract

Supplier: Promega

Wheat germ extract contains the cellular components necessary for protein synthesis (tRNA, ribosomes, initiation, elongation and termination factors).

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Trypsin/Lys-C mix, MS grade

Trypsin/Lys-C mix, MS grade

Supplier: Promega

Trypsin/Lys-C mix improves peptide digestion over trypsin alone, increasing peptide recovery and resulting in better protein quantitation by using standard digestion conditions.

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Arg-C Ultra Protease, Mass Spec Grade

Supplier: Promega

Fast, efficient and ultra-specific cysteine protease for use alone or with other proteases for bottom-up proteomics.

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Uniform latex particles for immobilisation of biomolecules, PL-Latex

Supplier: VARIAN

Uniform latex particles are used in a wide range of applications that exploit their physico-chemical properties in sophisticated immunoassay formats, both in medicine and veterinary science research.

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