You searched for: Protein Expression
Protein expression systems make it very easy to sensitively detect various proteins used widely in bioanalytics and molecular biology. Fluorophores or certain vitamins are used to tag antibodies or other proteins, and these labeled molecules can then be purified or detected using other solutions such as streptavidin or monomeric avidin. These protein expression systems are optimized for the rapid synthesis of functionally active proteins.
ProLite™ His-Tag Protein Gel Staining Kit
Supplier: AAT BIOQUEST
This kit requires very little hands-on time, allowing for rapid protein expression screening in a variety of gel types using an imaging equipment with a standard FITC filter.
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Autoinduction systems, Overnight Express™ Instant LB and TB Media
Supplier: Merck Millipore (Novagen)
The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.
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Alum adjuvant, AlumVax phosphate
Supplier: OZ BIOSCIENCES
AlumVax phosphate is a wet gel (colloidal) of aluminium phosphate 2%. Aluminium salts are the most common adjuvants used in approved prophylactic vaccines because of their excellent safety profile and ability to enhance the protective humoral immune response.
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Freund's incomplete adjuvant, IFAVax
Supplier: OZ BIOSCIENCES
Incomplete Freund’s Adjuvant (IFA) is a water-in-oil emulsion that is similar to the complete Freund’s Adjuvant (CFA) without addition of heat-killed mycobacteria (Mycobacterum butyricum).
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Squalene emulsion, SqualVax
Supplier: OZ BIOSCIENCES
SqualVax is an oil-in-water emulsion made of squalene droplets in a continuous aqueous phase. It is an important advantage over alternative oils that have been used in emulsion adjuvants, like Freund’s adjuvant that contains mineral oil (paraffin oil) and has long term persistence in organisms.
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Cell-free protein expression kits, 1-step human coupled IVT kit - DNA
Supplier: Thermo Fisher Scientific
The 1-Step Human Coupled IVT Kit – DNA is a mammalian in vitro translation (IVT) system based on HeLa cell lysates. The kit contains all of the cellular components required for protein synthesis, including ribosomes, initiation factors, elongation factors and tRNA. When supplemented with the included proprietary accessory proteins, reaction mix and a DNA template cloned into the Thermo Scientific pT7CFE1-based vector, this system can synthesise protein for up to 6 hours. The kit includes a pT7CFE-CHis vector for ease of use in downstream applications.
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Freund's complete adjuvant, CFAVax
Supplier: OZ BIOSCIENCES
Complete Freund’s Adjuvant (CFA) is a water-in-oil emulsion containing 1 mg per ml heat-killed Mycobacterium tuberculosis.
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Freund's complete adjuvant, Imject™
Supplier: Thermo Fisher Scientific
Imject™ Freund's Complete Adjuvant (FCA) is a popular non specific stimulator of the immune response for use in antibody production immunisation protocols.
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Freund's incomplete adjuvant, Imject™
Supplier: Thermo Fisher Scientific
Imject™ Freund's Incomplete Adjuvant (FIA) is a popular non specific stimulator of the immune response for use in antibody production immunisation protocols.
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Alum adjuvant, AlumVax hydroxide
Supplier: OZ BIOSCIENCES
AlumVax hydroxide is a wet gel (colloidal) of aluminium hydroxide 2%. It is the most common adjuvant used in approved prophylactic vaccines because of its excellent safety profile and ability to enhance the protective humoral immune response.
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Autoinduction systems, Overnight Express™ Autoinduction System 2
Supplier: Merck Millipore (Novagen)
The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.
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Autoinduction systems, Overnight Express™ NMR Media
Supplier: Merck Millipore (Novagen)
The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.
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Autoinduction systems, Overnight Express™ Autoinduction System 1
Supplier: Merck Millipore (Novagen)
The Overnight Express™ Autoinduction Systems enable regulated protein expression in E. coli, without monitoring the culture or adding inducer during cell growth.
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Rapid mouse antibody isotyping kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Rapid isotyping kit is a 5-minute lateral-flow assay with ELISA sensitivity for class and subclass determination of monoclonal antibodies.
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Freund's incomplete adjuvant, MP Biomedicals
Supplier: MP Biomedicals
Freund's incomplete adjuvant mixture contains mineral oil and Arlacel A.
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Freund's complete adjuvant, MP Biomedicals
Supplier: MP Biomedicals
Freund's complete adjuvant is made by a modification of Freund's technique, in which 25 mg of Mycobacterium is suspended in a mixture of 7,5 ml Arlacel A and 42,5 ml paraffin oil.
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High yield wheat germ protein expression system, TNT® SP6
Supplier: Promega
The TNT® SP6 high yield wheat germ protein expression system contains optimised wheat germ extract in a single-tube, coupled transcription/translation system that allows you to express proteins in only two hours from plasmid or PCR-generated templates.
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High yield protein expression system, S30 T7
Supplier: Promega
S30 T7 high yield protein expression system can be used to express up to 500 μg/ml of protein in 1 hour from plasmid vectors containing a T7 promoter and a ribosome binding site.
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T7 insect cell extract protein expression system, TNT®
Supplier: Promega
The TnT® T7 Insect Cell Extract Protein Expression System is a convenient, quick, single-tube, coupled transcription/translation system for the cell-free expression of proteins using an extract derived from the Spodoptera frugiperda Sf21 cell line.
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Regulated mammalian expression system
Supplier: Promega
Regulated mammalian expression system is based on a novel on/off switch that relies on the rapid and sensitive modulation by coumarin-related compounds of a chimeric transactivator protein.
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T7 quick protein expression system, for PCR, TNT®
Supplier: Promega
The TnT® T7 Quick for PCR is a simple, single-tube coupled transcription/translation system designed for optimum protein expression directly from PCR templates.
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Quick coupled transcription/translation system, TnT®
Supplier: Promega
Quick Coupled Transcription/Translation System provides convenient, single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression.
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Coupled Reticulocyte Lysate Systems, TnT®
Supplier: Promega
TnT® Coupled Reticulocyte Lysate System offers researchers an alternative for eukaryotic cell-free protein expression. It greatly simplifies the process and reduces the time required to obtain in vitro translation results.
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Luciferase SP6/T7 control DNAs
Supplier: Promega
The Luciferase SP6 and T7 Control DNAs are used as functional controls in the TNT® Quick Coupled and TNT® Coupled transcription/translation systems.
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Canine pancreatic microsomal membranes
Supplier: Promega
Microsomal vesicles are used to study cotranslational and initial posttranslational processing of proteins. Processing events such as signal peptide
cleavage, membrane insertion, translocation and core glycosylation can be examined by the translation of the appropriate mRNA in vitro in the presence of these microsomal membranes.
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E. coli T7 S30 Extract System for Circular DNA
Supplier: Promega
The E. coli T7 S30 Extract System for Circular DNA simplifies the transcription/translation of DNA sequences cloned in plasmid or λ vectors containing a T7 promoter by providing an extract that contains T7 RNA polymerase for transcription and all components needed for translation. The investigator only supplies cloned DNA containing a T7 promoter and a ribosome binding site.
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Rapid translation system (RTS), wheat germ kits
Supplier: biotechrabbit
The Rapid Translation System (RTS) is a scalable in vitro protein expression platform that produces large amounts of protein for characterisation studies, functional assays, or structural analysis. RTS enables both transcription and translation utilising High-Yield (HY) and continuous-exchange, cell-free (CECF) technologies, with systems based on bacterial (E. coli) or eukaryotic (wheat germ) cell lysates. RTS offers the advantages of a cell-free protein expression system, whilst eliminating the need for laborious up and downstream steps typically associated with cell-based expression systems.
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Rapid translation system (RTS), E. coli disulfide and fab kits
Supplier: biotechrabbit
The Rapid Translation System (RTS) is a scalable in vitro protein expression platform that produces large amounts of protein for characterisation studies, functional assays, or structural analysis. RTS enables both transcription and translation utilising High-Yield (HY) and continuous-exchange, cell-free (CECF) technologies, with systems based on bacterial (E. coli) or eukaryotic (wheat germ) cell lysates. RTS offers the advantages of a cell-free protein expression system, whilst eliminating the need for laborious up and downstream steps typically associated with cell-based expression systems.
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Cell-free protein synthesis, rapid translation system (RTS) linear template kits
Supplier: biotechrabbit
Expression kits for PCR-based generation of linear templates.
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Rapid translation system (RTS), E. coli high yield kits
Supplier: biotechrabbit
The Rapid Translation System (RTS) is a scalable in vitro protein expression platform that produces large amounts of protein for characterisation studies, functional assays, or structural analysis. RTS enables both transcription and translation utilising High-Yield (HY) and continuous-exchange, cell-free (CECF) technologies, with systems based on bacterial (E. coli) or eukaryotic (wheat germ) cell lysates. RTS offers the advantages of a cell-free protein expression system, whilst eliminating the need for laborious up and downstream steps typically associated with cell-based expression systems.