Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

ISA (ionic strength adjustment solution: 1 M KCl), Supelco®

Supplier: Merck

Solution used to establish a constant ionic strength in a sample solution containing e.g. calcium for det. with ISE.

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Buffer, TBE solution 10X concentrate (TRIS-borate-EDTA buffer), Millipore®

Supplier: Merck

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Buffer solutions, according to EP and USP, Certipur®

Supplier: Merck

Buffer solutions intended for extremely precise calibration, qualification and monitoring of pH instruments, and for testing other buffer solutions.

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Standard reference materials and buffer solutions, CertiPUR®, Supelco®

Supplier: Merck

Materials for the precise calibration and monitoring of pH instruments.

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Buffer solutions, ready to use, referenced at 20 °C, Certipur®, Supelco®

Supplier: Merck

Materials for the precise calibration and monitoring of pH meters.

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Triethylammonium bicarbonate buffer, for HPLC, LiChropur™, Supelco®

Supplier: Merck

1M Buffer, also for use in ion-exchange chromatography and electrophoresis.

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Phosphate buffer solution, BioUltra, Supelco®

Supplier: Merck

Phosphate buffer, BioUltra, >0,044 M Na₂HPO₄, >0,022 M NaH₂PO₄, pH value: 7,2

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VWR®, Tris buffer solution, pH 8 (1 mol/L) for biotechnology

Supplier: VWR Chemicals

Tris (hydroxymethyl) aminomethane.

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VWR® Buffer Tablets for Haematology

Supplier: VWR Chemicals

These tablets provide a rapid and convenient method of preparing a buffer solution.

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VWR® Transfer Buffer Concentrate, CAPS 10X

Supplier: VWR Chemicals

For transfer of protein to membranes for sequencing or blotting.

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VWR®, MOPS 10X buffer for biotechnology

Supplier: VWR Chemicals

MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.

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J.T.Baker® Cell Lysis Solution, Biotech Reagent

Supplier: Avantor

J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

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