Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Fixation Buffer
Supplier: Stemcell Technologies
Ideal for fixing cells prior to permeabilisation, fixation buffer can be used with Intracellular Permeabilisation Buffer (10X) to conduct intracellular staining for flow cytometry. This fixation buffer can also be used to preserve fluorescently labeled cells prior to analysis by flow cytometry.
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HEPES Buffer Solution (1 M)
Supplier: Stemcell Technologies
HEPES buffer solution is a biological buffer used in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture and degree of chemical definition required for the cell culture application.
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EasySep™ Red Blood Cell Lysis Buffer
Supplier: Stemcell Technologies
EasySep™ Red blood cell lysis buffer is for use as directed with EasySep™ or RoboSep™ whole blood kits for the lysis of red blood cells prior to separation from whole blood.
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Dulbecco's Phosphate Buffered Saline (DPBS), with 2% Fetal Bovine Serum
Supplier: Stemcell Technologies
Dulbecco's Phosphate Buffered Saline with 2% fetal bovine serum is recommended for use as a buffer to dilute or wash nucleated cells.
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Buffer, TRIS-glycine-SDS solution 10X concentrate (TGS buffer)
Supplier: Lonza
Running Buffer, TRIS-glycine-SDS buffer solution 10X concentrate (TGS buffer)
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RoboSep™-C PBS/EDTA Buffer
Supplier: Stemcell Technologies
PBS buffer containing EDTA.
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Hanks' Balanced Salt Solution, Modified without Ca++ and Mg++
Supplier: Stemcell Technologies
Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.
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Hanks' Balanced Salt Solution with 10 mM HEPES, without Phenol Red
Supplier: Stemcell Technologies
Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.
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