Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Buffer, TRIS solution pH 7,2 (1 mol/L), Sigma-Aldrich®
Supplier: Merck
Cloning Buffer, TRIS buffer solution pH 7,2 (1 mol/l)
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Glycine buffer, Sigma-Aldrich®
Supplier: Merck
For use in the enzymatic determination of lactate using lactic dehydrogenase.
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pH buffer solutions, certified (DKD)
Supplier: Mettler - Toledo
DKD certified buffer solution, pH value: 10,00, 6× 250 ml
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pH buffer solutions
Supplier: Mettler - Toledo
Technical buffer solution, 30×20 ml, pH value: 2,00
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Buffer, TRIS-glycine solution 10X concentrate (TG buffer), Sigma-Aldrich®
Supplier: Merck
Electrophoresis, Transfer buffer, TRIS-glycine buffer solution 10X concentrate (TG buffer)
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pH buffers, for LAQUAtwin meters
Supplier: HORIBA
pH 4,01 buffer (6× 14 ml bottles per pack), Bottle
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Technical pH buffer solutions, HI 5000 series
Supplier: Hanna
pH Buffer solution, pH value: 1,00, ±0,01, Reference temperature: 25 °C, Plastic bottle
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Redox buffer solutions
Supplier: Mettler - Toledo
Redox buffer solutions are used for verification of all common redox sensors.
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Buffer, sodium acetate buffer solution pH 7 (3 mol/L), Sigma-Aldrich®
Supplier: Merck
Cloning Buffer, Sodium acetate buffer solution pH 7 (3 mol/l)
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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L), Sigma-Aldrich®
Supplier: Merck
Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)
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RoboSep™ Buffer 2
Supplier: Stemcell Technologies
RoboSep™ buffer 2 is recommended for select EasySep™ cell separation protocols.
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Dulbecco's Phosphate Buffered Saline (D-PBS) without Ca++ and Mg++
Supplier: Stemcell Technologies
Dulbecco’s phosphate-buffered saline (D-PBS) can be used as a temporary diluting, washing, irrigating or transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media and provides cells with a source of water and essential inorganic ions.
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Ammonium Chloride Solution
Supplier: Stemcell Technologies
Ammonium chloride solution is recommended for the lysis of red blood cells (RBCs) in preparations of human and mouse peripheral blood, spleen or bone marrow cells. It is buffered and optimised for gentle lysis of erythrocytes, with minimal effect on leukocytes. It does not contain a fixative agent, therefore leukocytes are viable following RBC lysis.
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Intracellular Permeabilisation Buffer (10X)
Supplier: Stemcell Technologies
Ideal for the permeabilisation of fixed cells during labeling of cytokines and other cytoplasmic antigens. Intracellular Permeabilisation Buffer (10X) is uniquely formulated for optimal resolution and to reduce background and is suitable for use with fluorochrome-conjugated antibodies for flow cytometry.
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Hanks' Balanced Salt Solution, Modified without Ca++ and Mg++
Supplier: Stemcell Technologies
Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.
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Hanks' Balanced Salt Solution with 10 mM HEPES, without Phenol Red
Supplier: Stemcell Technologies
Hanks’ Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source. Selection of suitable nutrient medium is dependent on type of cell, culture conditions, and degree of chemical definition required for the cell culture application.
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#: 20864.320
Supplier: VWR Chemicals
#: 83640.320
Supplier: VWR Chemicals
#: 31770.294
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck
#: 83645.320
Supplier: VWR Chemicals