Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Gram Negative lysis buffer

Supplier: G-Biosciences

Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.

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G-Biosciences TBE-Urea Sample Buffer

Supplier: G-Biosciences

Loading buffer to resolve single-stranded DNA oligos or RNA into sharp, distinct bands.

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Phosphate Buffered Saline

Supplier: THERMO FISHER DIAGNOSTICS

Cell culture buffer, Phosphate buffered saline tablets

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Tris-Borate-EDTA (TBE) buffer, 10X

Supplier: PanReac AppliChem

10X concentrated solution of 0,89 M Tris, 0,89 M boric acid and 0,02 M EDTA.Na₂, pH 8,3 ±0,2.

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Mini flip-top vial with 9 ml buffered peptone water broth, BPPFV9BPW

Supplier: Neogen

The 3M™ Mini Flip-Top Vial with 9 ml Buffered Peptone Water Broth is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.

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Mini flip-top vial with 9 ml maximum recovery diluent, BPPFV9MRD

Supplier: Neogen

The 3M™ Mini Flip-Top Vial with 9 ml Maximum Recovery Diluent is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.

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Buffer, sodium acetate buffer solution pH 5,2 (3 mol/L)

Supplier: Cayman Chemical

Cloning Buffer, Sodium acetate buffer solution pH 5,2 (3 mol/l)

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Fixation/Permeabilisation, (buffer)

Supplier: Enzo Life Sciences

Fixation/Permeabilization, (buffer)

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Tris Borate EDTA buffer, for molecular biology, 10x, DNAse, RNAse and Protease free, pH 8.3

Supplier: Thermo Fisher Scientific

Running Buffer, TBE buffer solution 10X concentrate (TRIS-borate-EDTA buffer)

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Extraction reagents

Supplier: Enzo Life Sciences

Concentrated lysis buffer for preparation of cell and tissue extracts.

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Nuclear Extraction Kit, Abcam

Supplier: Abcam

This Nuclear Extraction Kit is formulated for the quick and simple isolation of nuclear and cytoplasmic fractions from cultured cells and tissue homogenates that can be used successfully in our transcription factor assay kits.

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Buffer, EDTA buffer solution pH 8,5 (0,5 mol/L)

Supplier: G-Biosciences

A DNase and RNase free solution of 0.5M EDTA.

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Opti-Klear™ Live Cell Imaging Buffer 1X, Abcam

Supplier: Abcam

Hepes based imaging buffer formulated to lower background fluorescence and maintain proper pH and osmolarity for hours at 37°C. ab275938 provides an energy source and preserves fluorescent signals.