You searched for: Assays
An assay is an investigative procedure in laboratory medicine, pharmacology, environmental biology, and molecular biology for qualitatively assessing or quantitatively measuring the presence or functional activity of the analyte, or the entity being targeted by the assay. Generally, assays involve biological materials or phenomena which tend to be intrinsically more complex in composition or in behavior than what can be handled by traditional chemical analysis and titration.
Screen Quest™ Fluo-4 No Wash Calcium Assay Kit
Supplier: AAT BIOQUEST
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).
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COX Colorimetric Inhibitor Screening Assay Kit
Supplier: Cayman Chemical
A colorimetric assay for the screening of isozyme-specific inhibitors.
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Mitochondria/cytosol fractionation kit
Supplier: ENZO LIFE SCIENCES
For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.
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Live bacterial gram stain kit
Supplier: Biotium
Wheat germ agglutinin binds specifically to N-acetylglucosamine in the peptidoglycan layer of gram-positive bacteria. The Live Bacterial Gram Stain Kit contains wheat germ agglutinin (WGA) conjugated to CF™594 to stain the surface of gram-positive cells with red fluorescence. The kit also includes DAPI to stain the DNA of all bacteria with blue fluorescence. Gram-positive bacteria stained using this kit will show a fluorescent blue interior and fluorescent red cell surface, while gram-negative bacterial will be stained with blue fluorescence only.
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Quantitative peroxide assay kit (aqueous), Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Quantitative Peroxide Assay Kit (Aqueous) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.
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Bacterial viability and gram stain kit
Supplier: Biotium
The Bacterial Viability and Gram Stain Kit contains three components: CF™488A-WGA, DAPI, and EthD-III solutions for distinguishing between gram-negative and gram-positive, as well as live versus dead bacteria.
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Catalase fluorometric detection kit
Supplier: ENZO LIFE SCIENCES
The Catalase fluorometric detection kit provides a higher throughput sensitive method for measuring catalase activity in just one hour. This is a homogeneous mix-and-read assay that enables you to monitor multiple time points for a kinetic read. Positive control is provided.
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Cell Meter™ Caspase 8 Activity Apoptosis Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
The Cell Meter™ assay kits are a set of tools for monitoring cellular functions.
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Mito-ID® Membrane potential cytotoxicity kit for microplates
Supplier: ENZO LIFE SCIENCES
A Real-time Mitochondrial membrane Potential Assay with Superior Sensitivity. Suitable for high-throughput applications.
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Screen Quest™ 10X Calcium Assay Buffer with Phenol Red Plus™
Supplier: AAT BIOQUEST
Ready-to-use buffer that is optimised for calcium assays.
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ADAM17 fluorometric drug discovery kit
Supplier: ENZO LIFE SCIENCES
Designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide. The ADAM17 Fluorometric (also known as fluorimetric) Drug Discovery Kit is a complete assay system designed to screen ADAM17 (TACE) inhibitors using a quenched fluorogenic peptide: Mca-PLAQAV-Dpa-RSSSR-NH2. Mca fluorescence is quenched by the Dpa group until cleavage by proteases separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of ADAM17, a potential therapeutic target. The compound GM6001 is also included as a prototypic control inhibitor.
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LYSO-ID® red cytotoxicity kit (GFP-CERTIFIED®) for microplates
Supplier: ENZO LIFE SCIENCES
A Rapid, Quantitative and HTS‐compatible Live Cell Cytotoxicity Assay
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Cell Meter™ Intracellular GSH Assay Kit *Optimised for Flow Cytometry*
Supplier: AAT BIOQUEST
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.
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Cell Meter™ Caspase 3/7 Activity Apoptosis Assay Kit *Blue Fluorescence*
Supplier: AAT BIOQUEST
Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.
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Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Blue Fluorescence*
Supplier: AAT BIOQUEST
Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions.
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Screen Quest™ TR-FRET No Wash cAMP Assay Kits
Supplier: AAT BIOQUEST
Screen Quest™ TR-FRET No Wash cAMP Assay Kit provides a convenient assay method for monitoring the activation of adenylyl cyclase in G-protein coupled receptor systems.
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Live or Dead™ Yeast Viability Assay Kit
Supplier: AAT BIOQUEST
Differentiates live yeast with intact membranes will stain fluorescent green, and dead yeast with damaged membranes will stain fluorescent red.
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PhosphoWorks™ Fluorimetric ATP Assay Kit
Supplier: AAT BIOQUEST
Adenosine triphosphate (ATP) plays a fundamental role in cellular energetics, metabolic regulation and cellular signaling.
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Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Green Fluorescence*
Supplier: AAT BIOQUEST
The Cell Meter™ assay kits are a set of tools for monitoring cellular functions.
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cAMP and cGMP conjugates
Supplier: Biotium
Conjugates for probing cAMP receptors or measuring cGMP in immunoassays.
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sPLA2 activity kit
Supplier: ENZO LIFE SCIENCES
For the quantitative determination of sPLA2 in culture supernatants, cell lysates, and buffers from any species.
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Hydrogen peroxide colorimetric detection kit
Supplier: ENZO LIFE SCIENCES
Hydrogen peroxide colorimetric detection kit provides a fast and higher throughput 96-well plate based homogeneous assay for the measurement of hydrogen peroxide in a wide variety of samples.
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ViaFluor® 647 live cell microtubule stain 1000X in DMSO
Supplier: Biotium
Stain microtubules in live cells with far-red fluorescence.
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Autophagy detection tests, CYTO-ID®
Supplier: ENZO LIFE SCIENCES
CYTO-ID can monitor autophagy in live cells and autophagic flux in lysosomally inhibited live cells using a novel dye that selectively labels accumulated autophagic vacuoles.
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FLUOR DE LYS® HDAC6 fluorometric drug discovery kit
Supplier: ENZO LIFE SCIENCES
A Fluor-de-Lys® fluorescent assay system. The HDAC6 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC6 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The Fluor de Lys® HDAC6 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.
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Firefly luciferase glow assay kit, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of firefly luciferase reporters for protein expression assays.
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CytoScan™-fluoro cytotoxicity assay kit
Supplier: G-Biosciences
The CytoScan™-fluoro Cytotoxicity assay is based on the quantification of cellular lactate dehydrogenase (LDH) released when cells are damaged or under stress. Lactate dehydrogenase released into the culture medium is measured with a diaphorase coupled enzymatic assay that results in the conversion of a non-fluorescent compound (resazurin) to a fluorescent compound (resorufin), which is measured using a fluorometer. The assay detects even low level damage to cell membrane not detected with other methods.
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Cell Meter™ Caspase 9 Activity Apoptosis Assay Kit *Red Fluorescence*
Supplier: AAT BIOQUEST
Our Cell Meter™ assay kits are a set of tools for monitoring cellular functions.
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Auto-Ubiquitinylation Kit
Supplier: ENZO LIFE SCIENCES
This E3 ligase auto-ubiquitinylation kit enables proteins to be tested for ubiquitin E3 ligase activity through assessment of their ability to under auto-ubiquitinylation. Utilizing the first three steps in the ubiquitin cascade the kit facilitates ubiquitinylation of known or putative E3 ligase enzymes followed by Western blot analysis using the highly sensitive reagents provided or using antibodies to the specific protein of interest (user supplied). A high integrity ubiquitin E3 ligase enzyme is also provided for use as a positive control.