You searched for: Assays

Supplier: Biotium

AccuGreen™ kit is specific for dsDNA and can quantify DNA samples in the range of 0,1 to 10 ng/μl.

Supplier: Thermo Fisher Scientific

The bradford protein assay is the preferred colorimetric assay for quantifying total protein concentration. The Pierce™ coomassie plus protein assay is a ready-to-use, reducing agent-compatible, improved bradford assay reagent to quickly measure (A595 nm) total protein concentration compared to a protein standard.

Supplier: SIGMA ALDRICH MICROSCOPY

Bicinchoninic acid is used for the determination of protein concentration. It is responsible for the making of intense purple complex with cuprous ion (Cu+) in the presence of alkaline conditions. The amount of Cu+ is in relation with the protein concentration.

Supplier: Cayman Chemical

A sensitive tool for measuring free fatty acids in plasma, serum, and urine.

Supplier: ENZO LIFE SCIENCES

Live cell fluorescent labelling used for a long term cell viability, cytotoxicity, cell adhesion, cell migration, and cell-cell fusion studies.

Supplier: Thermo Fisher Scientific

Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

Supplier: G-Biosciences

The Phosphatase Assay Kit is designed to measure the activity of phosphatases in biological samples and to screen for agonists and inhibitors of phosphatases. The Phosphatase Assay Kit uses para-nitrophenyl phosphate (pNPP), a chromogenic substrate for most phosphatases, including alkaline phosphatases, acid phosphatases, protein tyrosine phosphatases and serine/threonine phosphatases.

Supplier: Biotium

Apoptosis and necrosis are two processes by which cells die. Apoptosis is an active, regulated disassembly of the cell from within. During apoptosis, phosphatidylserine is translocated from the inner to the outer surface of the cell, allowing the dying cell to be engulfed by phagocytic cells. Annexin V is a 35 kD Ca2+-dependent phospholipid binding protein with a high affinity for phosphatidylserine.

Supplier: AAT BIOQUEST

Cell Meter™ flow cytometric calcium assay kit provides fluorescence-based assays for detecting intracellular calcium mobilisation using a flow cytometer.

Supplier: AAT BIOQUEST

Our Cell Meter™ assay kits are a set of tools for monitoring cell viability.

Supplier: AAT BIOQUEST

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

Supplier: AAT BIOQUEST

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

Supplier: OZ BIOSCIENCES

Bradford Protein Assay Kit (B-Pak) is a straightforward and rapid kit for determining the concentration of proteins in solution. This ready to use kit is provided with 1X reagent and 2 sets of BSA standards which means no dilution, filtration or calculation required. B-Pak is provided with adapted protocols for all experimental conditions – micro and macroassays.

Supplier: Thermo Fisher Scientific

The Compat-Able™ Reagents remove salts, detergents, reducing agents and other substances from protein samples to eliminate interference with protein assays. The protocol selectively precipitates all of the protein, thereby allowing the non-protein components to be easily decanted and removed. The purified, precipitated protein is redissolved in water, making it ready for accurate determination of the protein concentration by standard protein assay methods. The procedure is accomplished in less than 10 min with just a few simple steps. The Compat-Able™ Preparation Set is available conveniently bundled with the Pierce BCA™ Protein Assay Kit.

Supplier: AAT BIOQUEST

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

Supplier: OZ BIOSCIENCES

BCA Protein Assay kit (BCA-PAK) is useful for colorimetric detection and quantification of total protein content, even in the presence of detergents.

Supplier: ENZO LIFE SCIENCES

The LSD1 Fluorimetric Drug Discovery Kit provides human recombinant LSD1 and all reagents for measuring its activity in a sensitive, real-time fluorescent assay.

Supplier: Biotium

The Bacterial Viability and Gram Stain Kit contains three components: CF™488A-WGA, DAPI, and EthD-III solutions for distinguishing between gram-negative and gram-positive, as well as live versus dead bacteria.

Supplier: Thermo Fisher Scientific

Pierce™ Quantitative Peroxide Assay Kit (Lipid) detects and measures hydrogen peroxide levels in biological samples using an iron and xylenol orange* (XO) reagent.

Supplier: AAT BIOQUEST

In the presence of inorganic phosphate MESG is converted to 2-amino-6-mercapto-7-methlpurine by purine nucleoside phosphorylase (EC 2.4.2.1) with absorption wavelength shift to red.

Supplier: ENZO LIFE SCIENCES

A Rapid, Quantitative and HTS‐compatible Live Cell Cytotoxicity Assay

Supplier: ENZO LIFE SCIENCES

The Myeloperoxidase fluorometric detection kit is a one-step no wash homogeneous activity assay that is adaptable to HTS applications. This kit offers highly sensitive fluorescent detection and the ability to monitor multiple time points to follow the kinetics of MPO activity.

Supplier: ENZO LIFE SCIENCES

Calcium dye

Supplier: G-Biosciences

CytoScan™ WST-1 Cell Proliferation Assay principle is based upon the reduction of the tetrazolium salt WST-1 to formazan by cellular dehydrogenases. The generation of the dark yellow coloured formazan is measured at 420 to 480 nm (optimal at 440 nm) and is directly correlated to cell number.

Supplier: AAT BIOQUEST

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR).

Supplier: Biotium

Nerve terminal probes are a series of fluorescent cationic styryl dyes developed to follow synaptic activity. These dyes have a lipophilic tail at one end and a hydrophilic cationically charged head group at the other end. The dyes vary in the number of carbons in the lipophilic tail (m) and the number of double bonds linking the two aromatic rings in the dye (n). AM dyes are similar to FM dyes, but contain an additional formaldehyde-fixable amino group in the cationic head.

Supplier: ENZO LIFE SCIENCES

Hydrogen peroxide colorimetric detection kit provides a fast and higher throughput 96-well plate based homogeneous assay for the measurement of hydrogen peroxide in a wide variety of samples.

Supplier: ENZO LIFE SCIENCES

The Hydrogen Peroxide Fluorometric Detection Kit provides fast results in just 10 minutes for the measurement of either hydrogen peroxide or peroxidase activity in a convenient one-step homogeneous assay.

Supplier: Biotium

Wheat germ agglutinin binds specifically to N-acetylglucosamine in the peptidoglycan layer of gram-positive bacteria. The Live Bacterial Gram Stain Kit contains wheat germ agglutinin (WGA) conjugated to CF™594 to stain the surface of gram-positive cells with red fluorescence. The kit also includes DAPI to stain the DNA of all bacteria with blue fluorescence. Gram-positive bacteria stained using this kit will show a fluorescent blue interior and fluorescent red cell surface, while gram-negative bacterial will be stained with blue fluorescence only.

Supplier: ENZO LIFE SCIENCES

Versatile tool for generation of ubiquitin-E2 thioesters.