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You searched for: Restriction Enzymes

DNA methylation analysis kit (MspI/HpaII), EpiJET™

Supplier: Thermo Fisher Scientific

5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression. The EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyse DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected. The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.

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Restriction enzymes, PstI

Restriction enzymes, PstI

Supplier: Promega

These restriction enzymes are capable of digesting DNA in 15 minutes or less. Recognises C TGCA˅G and G˄ACGT C.

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Restriction enzymes, AluI

Restriction enzymes, AluI

Supplier: Promega

These AluI restriction enzyme recognises the cut site of AG˅CT and TC˄GA.

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Restriction enzymes, TaqI

Restriction enzymes, TaqI

Supplier: Promega

The TaqI restriction enzyme recognises the cut site T˅CG A and A GC˄T.

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Restriction enzymes, DdeI

Supplier: Promega

These DdeI restriction enzyme recognises the cut site C˅TNA G and G ANT˄C. Produces an ambiguous extension, which is difficult to ligate.

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Restriction enzymes, PvuII

Supplier: Promega

These PvuII restriction enzyme recognises the cut site CAG˅CTG and GTC˄GAC. Star activity may be observed with glycerol concentrations >12% or with enzyme: DNA ratios >25 U/μg.

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Restriction enzymes, TaqI, HC

Supplier: Promega

The TaqI restriction enzyme recognises the cut site T˅CG A and A GC˄T.

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Restriction enzymes, DpnI

Restriction enzymes, DpnI

Supplier: Promega

These DpnI restriction enzyme recognises the cut size of GmeA˅TC and CT˄meAG. Requires N6-methylation of the adenine residue for activity.

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Restriction enzymes, XhoI

Restriction enzymes, XhoI

Supplier: Promega

The XhoI restriction enzymes are capable of digesting DNA in 15 minutes or less. It recognises the cut site C˅TCGA G and G AGCT˄C. Genome qualified to ensure optimal performance in genomic analysis applications

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Restriction enzymes, XbaI

Restriction enzymes, XbaI

Supplier: Promega

The Xbal restriction enzyme is capable of digesting DNA in 15 minutes or less. It recognises T˅CTAG A and A GATC˄T. Genome qualified to ensure optimal performance in genomic analysis applications.

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Restriction enzymes, SmaI

Restriction enzymes, SmaI

Supplier: Promega

These SmaI restriction enzymes are genome qualified to ensure optimal performance in genomic analysis applications. Recognises CCC˅GGG and GGG˄CCC.

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Restriction enzymes, HaeIII

Restriction enzymes, HaeIII

Supplier: Promega

The HaeIII restriction enzyme is capable of digesting DNA in 15 minutes or less. It recognises the cut site GG˅CC and CC˄GG.

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Restriction enzymes, HaeIII, HC

Supplier: Promega

The HaeIII restriction enzyme is capable of digesting DNA in 15 minutes or less. It recognises the cut site GG˅CC and CC˄GG.

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Restriction enzymes, PvuI

Restriction enzymes, PvuI

Supplier: Promega

These PvuI restriction enzyme recognises the cut site of CG AT˅CG and GC˄TA GC. Ends generated by PvuI can be directly ligated to ends generated by SgfI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.

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Restriction enzymes, XhoI, HC

Supplier: Promega

The XhoI restriction enzymes are capable of digesting DNA in 15 minutes or less. It recognises the cut site C˅TCGA G and G AGCT˄C. Genome qualified to ensure optimal performance in genomic analysis applications

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Restriction enzymes, HinfI

Restriction enzymes, HinfI

Supplier: Promega

G at 37 °C in its own unique buffer.

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Restriction enzymes, EcoRV

Restriction enzymes, EcoRV

Supplier: Promega

TAG at 37 °C in its own unique buffer.

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Restriction enzymes, BanI

Supplier: Promega

G sites and cuts best at 37 °C in its own unique buffer. Exhibit 10-25% activity in the recommended buffer and reaction conditions.

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Restriction enzymes, BstEII

Supplier: Promega

G sites and cuts best at 60 °C in its own unique buffer. Exhibit 25 to 50% activity in the recommended buffer and reaction conditions.

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Restriction enzymes, SalI

Restriction enzymes, SalI

Supplier: Promega

G at 37 °C in its own unique buffer.

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Restriction enzymes, Eco47III

Supplier: Promega

CGA sites and cuts best at 37 °C in its own unique buffer.

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Restriction enzymes, BclI

Restriction enzymes, BclI

Supplier: Promega

T sites and cuts best at 50 °C in its own unique buffer. Exhibit 10 to 25% activity in the recommended buffer and reaction conditions.

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Restriction enzymes, SfiI

Supplier: Promega

NNN NCCGG at 37 °C in its own unique buffer.

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Restriction enzymes, StuI

Supplier: Promega

GGA sites and cuts best at 37 °C in its own unique buffer.

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Restriction enzymes, HindIII

Restriction enzymes, HindIII

Supplier: Promega

A at 37 °C in its own unique buffer.

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Restriction enzymes, Tru9 I

Supplier: Promega

T sites and cuts best at 37 °C in its own unique buffer.

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Restriction enzymes, Hsp92II

Supplier: Promega

GTAC sites and cuts best at 37 °C in its own unique buffer.

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Restriction enzymes, EcoRI

Restriction enzymes, EcoRI

Supplier: Promega

G at 37 °C in its own unique buffer.

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Restriction enzymes, HincII

Supplier: Promega

(T/C)TG at 37 °C in its own unique buffer.

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Restriction enzymes, KpnI

Restriction enzymes, KpnI

Supplier: Promega

CATG G sites and cuts best at 37 °C in its own unique buffer.

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