You searched for: Restriction Enzymes
DNA methylation analysis kit (MspI/HpaII), EpiJET™
Supplier: Thermo Fisher Scientific
5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression. The EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyse DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected. The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.
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Restriction enzymes, PstI
Supplier: Promega
These restriction enzymes are capable of digesting DNA in 15 minutes or less. Recognises C TGCA˅G and G˄ACGT C.
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Restriction enzymes, AluI
Supplier: Promega
These AluI restriction enzyme recognises the cut site of AG˅CT and TC˄GA.
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Restriction enzymes, TaqI
Supplier: Promega
The TaqI restriction enzyme recognises the cut site T˅CG A and A GC˄T.
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Restriction enzymes, DdeI
Supplier: Promega
These DdeI restriction enzyme recognises the cut site C˅TNA G and G ANT˄C. Produces an ambiguous extension, which is difficult to ligate.
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Restriction enzymes, PvuII
Supplier: Promega
These PvuII restriction enzyme recognises the cut site CAG˅CTG and GTC˄GAC. Star activity may be observed with glycerol concentrations >12% or with enzyme: DNA ratios >25 U/μg.
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Restriction enzymes, TaqI, HC
Supplier: Promega
The TaqI restriction enzyme recognises the cut site T˅CG A and A GC˄T.
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Restriction enzymes, DpnI
Supplier: Promega
These DpnI restriction enzyme recognises the cut size of GmeA˅TC and CT˄meAG. Requires N6-methylation of the adenine residue for activity.
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Restriction enzymes, XhoI
Supplier: Promega
The XhoI restriction enzymes are capable of digesting DNA in 15 minutes or less. It recognises the cut site C˅TCGA G and G AGCT˄C. Genome qualified to ensure optimal performance in genomic analysis applications
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Restriction enzymes, XbaI
Supplier: Promega
The Xbal restriction enzyme is capable of digesting DNA in 15 minutes or less. It recognises T˅CTAG A and A GATC˄T. Genome qualified to ensure optimal performance in genomic analysis applications.
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Restriction enzymes, SmaI
Supplier: Promega
These SmaI restriction enzymes are genome qualified to ensure optimal performance in genomic analysis applications. Recognises CCC˅GGG and GGG˄CCC.
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Restriction enzymes, HaeIII
Supplier: Promega
The HaeIII restriction enzyme is capable of digesting DNA in 15 minutes or less. It recognises the cut site GG˅CC and CC˄GG.
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Restriction enzymes, HaeIII, HC
Supplier: Promega
The HaeIII restriction enzyme is capable of digesting DNA in 15 minutes or less. It recognises the cut site GG˅CC and CC˄GG.
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Restriction enzymes, PvuI
Supplier: Promega
These PvuI restriction enzyme recognises the cut site of CG AT˅CG and GC˄TA GC. Ends generated by PvuI can be directly ligated to ends generated by SgfI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.
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Restriction enzymes, XhoI, HC
Supplier: Promega
The XhoI restriction enzymes are capable of digesting DNA in 15 minutes or less. It recognises the cut site C˅TCGA G and G AGCT˄C. Genome qualified to ensure optimal performance in genomic analysis applications
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Restriction enzymes, HinfI
Supplier: Promega
G at 37 °C in its own unique buffer.
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Restriction enzymes, EcoRV
Supplier: Promega
TAG at 37 °C in its own unique buffer.
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Restriction enzymes, BanI
Supplier: Promega
G sites and cuts best at 37 °C in its own unique buffer. Exhibit 10-25% activity in the recommended buffer and reaction conditions.
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Restriction enzymes, BstEII
Supplier: Promega
G sites and cuts best at 60 °C in its own unique buffer. Exhibit 25 to 50% activity in the recommended buffer and reaction conditions.
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Restriction enzymes, SalI
Supplier: Promega
G at 37 °C in its own unique buffer.
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Restriction enzymes, Eco47III
Supplier: Promega
CGA sites and cuts best at 37 °C in its own unique buffer.
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Restriction enzymes, BclI
Supplier: Promega
T sites and cuts best at 50 °C in its own unique buffer. Exhibit 10 to 25% activity in the recommended buffer and reaction conditions.
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Restriction enzymes, SfiI
Supplier: Promega
NNN NCCGG at 37 °C in its own unique buffer.
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Restriction enzymes, StuI
Supplier: Promega
GGA sites and cuts best at 37 °C in its own unique buffer.
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Restriction enzymes, HindIII
Supplier: Promega
A at 37 °C in its own unique buffer.
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Restriction enzymes, Tru9 I
Supplier: Promega
T sites and cuts best at 37 °C in its own unique buffer.
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Restriction enzymes, Hsp92II
Supplier: Promega
GTAC sites and cuts best at 37 °C in its own unique buffer.
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Restriction enzymes, EcoRI
Supplier: Promega
G at 37 °C in its own unique buffer.
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Restriction enzymes, HincII
Supplier: Promega
(T/C)TG at 37 °C in its own unique buffer.
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Restriction enzymes, KpnI
Supplier: Promega
CATG G sites and cuts best at 37 °C in its own unique buffer.