You searched for: Restriction Enzymes

Supplier: Thermo Fisher Scientific

5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression. The EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyse DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected. The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.

Supplier: Promega

These restriction enzymes are capable of digesting DNA in 15 minutes or less. Recognises C TGCA^˅G and G_˄ACGT C.

Supplier: Promega

These AluI restriction enzyme recognises the cut site of AG^˅CT and TC_˄GA.

Supplier: Promega

The TaqI restriction enzyme recognises the cut site T^˅CG A and A GC_˄T.

Supplier: Promega

These DdeI restriction enzyme recognises the cut site C^˅TNA G and G ANT_˄C. Produces an ambiguous extension, which is difficult to ligate.

Supplier: Promega

These PvuII restriction enzyme recognises the cut site CAG^˅CTG and GTC_˄GAC. Star activity may be observed with glycerol concentrations >12% or with enzyme: DNA ratios >25 U/μg.

Supplier: Promega

The TaqI restriction enzyme recognises the cut site T^˅CG A and A GC_˄T.

Supplier: Promega

These DpnI restriction enzyme recognises the cut size of G^meA^˅TC and CT_˄^meAG. Requires N6-methylation of the adenine residue for activity.

Supplier: Promega

The XhoI restriction enzymes are capable of digesting DNA in 15 minutes or less. It recognises the cut site C^˅TCGA G and G AGCT_˄C. Genome qualified to ensure optimal performance in genomic analysis applications

Supplier: Promega

The Xbal restriction enzyme is capable of digesting DNA in 15 minutes or less. It recognises T^˅CTAG A and A GATC_˄T. Genome qualified to ensure optimal performance in genomic analysis applications.

Supplier: Promega

These SmaI restriction enzymes are genome qualified to ensure optimal performance in genomic analysis applications. Recognises CCC^˅GGG and GGG_˄CCC.

Supplier: Promega

The HaeIII restriction enzyme is capable of digesting DNA in 15 minutes or less. It recognises the cut site GG^˅CC and CC_˄GG.

Supplier: Promega

The HaeIII restriction enzyme is capable of digesting DNA in 15 minutes or less. It recognises the cut site GG^˅CC and CC_˄GG.

Supplier: Promega

These PvuI restriction enzyme recognises the cut site of CG AT^˅CG and GC_˄TA GC. Ends generated by PvuI can be directly ligated to ends generated by SgfI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.

Supplier: Promega

The XhoI restriction enzymes are capable of digesting DNA in 15 minutes or less. It recognises the cut site C^˅TCGA G and G AGCT_˄C. Genome qualified to ensure optimal performance in genomic analysis applications

Supplier: Promega

G at 37 °C in its own unique buffer.

Supplier: Promega

TAG at 37 °C in its own unique buffer.

Supplier: Promega

G sites and cuts best at 37 °C in its own unique buffer. Exhibit 10-25% activity in the recommended buffer and reaction conditions.

Supplier: Promega

G sites and cuts best at 60 °C in its own unique buffer. Exhibit 25 to 50% activity in the recommended buffer and reaction conditions.

Supplier: Promega

G at 37 °C in its own unique buffer.

Supplier: Promega

CGA sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

T sites and cuts best at 50 °C in its own unique buffer. Exhibit 10 to 25% activity in the recommended buffer and reaction conditions.

Supplier: Promega

NNN NCCGG at 37 °C in its own unique buffer.

Supplier: Promega

GGA sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

A at 37 °C in its own unique buffer.

Supplier: Promega

T sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

GTAC sites and cuts best at 37 °C in its own unique buffer.

Supplier: Promega

G at 37 °C in its own unique buffer.

Supplier: Promega

(T/C)TG at 37 °C in its own unique buffer.

Supplier: Promega

CATG G sites and cuts best at 37 °C in its own unique buffer.