You searched for: Restriction Enzymes
DNA methylation analysis kit (MspI/HpaII), EpiJET™
Supplier: Thermo Fisher Scientific
5-Methylcytosine is a prominent epigenetic DNA modification which plays an important role in regulation of gene expression. The EpiJET™ DNA Methylation Analysis Kit (MspI/HpaII) uses the MspI and HpaII restriction enzymes to analyse DNA methylation status at a specific locus. Epi MspI and Epi HpaII are isoschizomers with differing sensitivities to CpG methylation. When the internal CpG in the 5’-CCGG-3’ tetranucleotide sequence is methylated, cleavage with Epi HpaII is blocked, but cleavage with Epi MspI is not affected. The Epi MspI and Epi HpaII enzymes are specially formulated for epigenetics studies to complete genomic DNA digestion in 1 hour.
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BspQ I Restriction Endonuclease
Supplier: Abnova
The restriction endonuclease is a restriction endonuclease BspQ I that can recognize specific sites and is produced under GMP standards and expressed recombinantly in Escherichia coli. Restriction endonucleases are widely used in various fields such as gene positioning and cloning. This enzyme cleaves DNA rapidly for efficient gene linearization.
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BSA I Restriction Endonuclease
Supplier: Abnova
The BSA I restriction endonuclease is a restriction endonuclease BsaI that can recognize specific sites expressed by Escherichia coli recombinantly. Restriction endonucleases are widely used in various fields such as gene positioning and cloning. This enzyme cleaves DNA rapidly for efficient gene linearization.
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Restriction enzymes, PstI
Supplier: Promega
These restriction enzymes are capable of digesting DNA in 15 minutes or less. Recognises C TGCA˅G and G˄ACGT C.
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Restriction enzymes, AluI
Supplier: Promega
These AluI restriction enzyme recognises the cut site of AG˅CT and TC˄GA.
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Restriction enzymes, TaqI, HC
Supplier: Promega
The TaqI restriction enzyme recognises the cut site T˅CG A and A GC˄T.
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Restriction enzymes, HinfI
Supplier: Promega
G at 37 °C in its own unique buffer.
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Restriction enzymes, EcoRV
Supplier: Promega
TAG at 37 °C in its own unique buffer.
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Restriction enzymes, HaeIII, HC
Supplier: Promega
The HaeIII restriction enzyme is capable of digesting DNA in 15 minutes or less. It recognises the cut site GG˅CC and CC˄GG.
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Restriction enzymes, HinfI, HC
Supplier: Promega
G at 37 °C in its own unique buffer.
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Restriction enzymes, PvuII
Supplier: Promega
These PvuII restriction enzyme recognises the cut site CAG˅CTG and GTC˄GAC. Star activity may be observed with glycerol concentrations >12% or with enzyme: DNA ratios >25 U/μg.
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Restriction enzymes, TaqI
Supplier: Promega
The TaqI restriction enzyme recognises the cut site T˅CG A and A GC˄T.
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Restriction enzymes, Mbo I
Supplier: Promega
sites and cuts best at 37 °C in its own unique buffer.
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Restriction enzymes, Spe I
Supplier: Promega
A sites and cuts best at 37 °C in its own unique buffer.
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Restriction enzymes, XhoI, HC
Supplier: Promega
The XhoI restriction enzymes are capable of digesting DNA in 15 minutes or less. It recognises the cut site C˅TCGA G and G AGCT˄C. Genome qualified to ensure optimal performance in genomic analysis applications
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Restriction enzymes, ClaI
Supplier: Promega
TA sites and cuts best at 37 °C in its own unique buffer.
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Restriction enzymes, EcoRI
Supplier: Promega
G at 37 °C in its own unique buffer.
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Restriction enzymes, BanI
Supplier: Promega
G sites and cuts best at 37 °C in its own unique buffer. Exhibit 10-25% activity in the recommended buffer and reaction conditions.
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Restriction enzymes, NotI, 10 U/μl
Supplier: Promega
CG sites and cuts best at 37 °C in its own unique buffer.
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Restriction enzymes, PvuI
Supplier: Promega
These PvuI restriction enzyme recognises the cut site of CG AT˅CG and GC˄TA GC. Ends generated by PvuI can be directly ligated to ends generated by SgfI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.
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Restriction enzymes, BstEII
Supplier: Promega
G sites and cuts best at 60 °C in its own unique buffer. Exhibit 25 to 50% activity in the recommended buffer and reaction conditions.
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Restriction enzymes, HincII
Supplier: Promega
(T/C)TG at 37 °C in its own unique buffer.
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Restriction enzymes, SmaI
Supplier: Promega
These SmaI restriction enzymes are genome qualified to ensure optimal performance in genomic analysis applications. Recognises CCC˅GGG and GGG˄CCC.
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Restriction enzymes, KpnI
Supplier: Promega
CATG G sites and cuts best at 37 °C in its own unique buffer.
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Restriction enzymes, ApaI, HC
Supplier: Promega
CCGG G at 37 °C in its own unique buffer.
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Restriction enzymes, SalI, HC
Supplier: Promega
G at 37 °C in its own unique buffer.
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Restriction enzymes, MspI
Supplier: Promega
C sites and cuts best at 37 °C in its own unique buffer.
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Restriction enzymes, NcoI
Supplier: Promega
C sites and cuts best at 37 °C in its own unique buffer.
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Restriction enzymes, NheI
Supplier: Promega
G sites and cuts best at 37 °C in its own unique buffer.
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Restriction enzymes, EcoRI, HC
Supplier: Promega
G at 37 °C in its own unique buffer.