You searched for: Nucleic Acid Purification Kits and Reagents

Supplier: Promega

PolyATtract® mRNA Isolation Systems use MagneSphere® technology to isolate mRNA rapidly and effectively from total RNA.

Supplier: Promega

The Maxwell® 16 Tissue DNA Purification Kit is used with the Maxwell® 16 instrument to provide an easy method for efficient, automated purification of genomic DNA from up to 50 mg of tissue samples. The purified DNA can be used directly in a variety of downstream applications including PCR, restriction enzyme digestion and agarose gel electrophoresis. The plunger action of the Maxwell® 16 instrument grinds solid tissue samples directly in lysis buffer in the prefilled reagent cartridge.

Supplier: Promega

The ReliaPrep RNA miniprep systems provide a fast and simple technique for preparation of intact total RNA from cultured cells or tissue in as little as 30 minutes from very small amounts of input material.

Supplier: Promega

The QuantiFluor® ONE dsDNA System provides a fluorescent double-stranded DNA-binding dye (504 nm Ex/531 nm Em) in an automation-friendly, "Add-and-Read" format for both dye and standard, simplifying DNA quantitation and speeding up workflow.

Supplier: Promega

Automated high-throughput extraction of cell-free DNA from plasma, serum, urine and CSF.

Supplier: Promega

The ReliaPrep gDNA Tissue Miniprep System provides a complete, ready-to-use method for purification of gDNA from up to 25 mg of tissue, a buccal swab, or a 1cm mouse tail snip, obtaining intact gDNA without the use of ethanol washes or precipitations.

Supplier: Promega

The Wizard® Genomic DNA Purification Kit provides a simple, solution-based method for isolation of DNA from white blood cells, tissue culture cells, animal tissue, plant tissue, yeast and gram-positive and gram-negative bacteria. DNA purified with this system is suitable for a variety of applications, including amplification, digestion with restriction endonucleases and membrane hybridisations such as southern and dot/slot blots.

Supplier: Promega

The PureYield™ plasmid Midiprep system is designed to isolate transfection quality plasmid DNA. The system provides a rapid method for purification of 100 to 200 μg of plasmid DNA from 50 ml bacteria culture. An alternative protocol allows purification of over 400 μg of high copy number plasmid from 250 ml of E. coli culture.

Supplier: Promega

Maxwell® RSC simplyRNA Blood Kit, used with the Maxwell RSC instrument, provides a simple method for automated RNA purification from 2,5 ml of fresh whole blood. The low elution volume is used to generate concentrated high quality RNA suitable for use in downstream applications such as quantitative RT-PCR, gene expression and translation assays.

Supplier: Promega

The E. coli S30 Extract System for Linear Templates allows successful transcription/translation of linear DNA templates. The investigator need only provide linear DNA containing a prokaryotic E. coli-like promoter (such as lacUV5, tac, λPL (con) and λ-PR). A ribosome binding site is required to direct the synthesis of proteins in vitro.

Supplier: Promega

The Wizard® SV Gel and PCR Clean-up System is designed to extract and purify DNA fragments of 100 bp to 10 kb from standard or low-melting agarose gels or to purify products directly from PCR and other common reactions, such as restriction digests. The purified DNA can be used for automated fluorescent DNA sequencing, cloning, labeling, restriction enzyme digestion or in vitro transcription/translation without further manipulation.

Supplier: Promega

The Wizard® Plus SV Minipreps DNA purification system is a silica membrane based system, provides a simple and reliable method for rapid isolation of plasmid DNA. Using the system, plasmid DNA can be purified from 1 to 10ml of overnight E.coli culture. The purified plasmid DNA can be used directly for automated fluorescent BigDye® terminator DNA sequencing as well as for other standard molecular biology techniques without further manipulation and also in vitro transcription reactions.

Supplier: Promega

The Wizard® SV 96 genomic DNA purification system provides a high-throughput, membrane based technique for consistent preparation of genomic DNA from cultured cells and tissue, including mouse tails. Amplifiable genomic DNA can be isolated from up to 5×10⁶ cells, 20 mg of tissue or up to 1,2 cm of a mouse tail tip without a centrifugation clearing step. With this system, genomic DNA is purified from cell lysates using 96-well vacuum filtration. Washing the bound DNA requires no disassembly of the manifold, and filtrate waste products are delivered directly to a vacuum trap, eliminating the need to empty waste collection trays.

Supplier: Promega

The PureYield™ plasmid Miniprep system is designed to rapidly isolate highly pure plasmid DNA. The system provides a rapid method for purification of up to 15 μg of plasmid DNA from 600 μl to 3 ml of bacteria culture.

Supplier: Promega

The ReliaPrep™ FFPE Total RNA Miniprep System optimises lysis conditions to reverse RNA modifications that can be introduced when extracting RNA from FFPE tissues.

Supplier: Promega

The Maxwell® 16 Mouse Tail DNA Purification Kit is used with the Maxwell® 16 instrument to extract DNA from mouse tails, ear punches and other tissue in 30 to 45 minutes. The easy, automated purification method gives consistent yield and purity.

Supplier: Promega

The ReliaPrep™ FFPE gDNA Miniprep System provides a complete, all inclusive method for purifying quality genomic DNA from formalin fixed paraffin-embedded tissue without using hazardous solvents or overnight digestion. The optimised lysis and binding conditions reverse modifications introduced by the fixation process, results in intact, amplifiable gDNA.

Supplier: Promega

The Maxwell® 16 LEV simplyRNA kits are for use with the Maxwell® 16 instrument configured with the low elution volume (LEV) high strength magnetic rod and plunger bar adaptor. This RNA purification procedure is a simple method with minimal lysate handling before automated purification on the Maxwell® 16 instrument. The low elution volume is used to generate concentrated high quality RNA suitable for use in downstream applications, such as quantitative RT-PCR.

Supplier: Promega

The resin-based Wizard® Plus Midipreps DNA Purification System provides a simple and reliable method for rapid isolation of plasmid DNA. Midipreps may be processed individually or in multiples with the Vac-Man® (20 sample capacity) or Vac-Man® Jr. (2 sample capacity) laboratory vacuum manifold. The purified plasmid can be used directly for automated fluorescent DNA sequencing and restriction digestion without further manipulation and also can be used for in vitro transcription reactions.

Supplier: Promega

The Wizard® SV 96 PCR clean up system is designed for high throughput purification of 100 bp to 10 kb PCR products from excess nucleotides, primers and primer dimers. The purified DNA can be used for automated fluorescent sequencing, cloning, labeling, restriction digestion or microarray analysis without further manipulation. The Wizard® SV 96 PCR clean-up system uses 96-well filtration without the need to disassemble the manifold.

Supplier: Promega

Maxwell® 16 Low Elution Volume (LEV) Blood DNA Kit is designed for optimal automated extraction of DNA from whole blood samples using the Maxwell® 16 instrument. Up to 300 μl of whole blood can be processed to yield up to 150 ng/μl. The Maxwell® 16 LEV Blood DNA Kit delivers buffy coat-like purity and concentration from whole blood, saving time and allowing automation. Used in combination with the Maxwell® 16 instrument.

Supplier: Promega

The Wizard® Plus Maxipreps DNA purification system provides a simple and rapid resin based batch column method for purification of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. This system requires only a centrifuge, a vacuum source and a vacuum manifold. This system typically yields 300 μg to 1 mg of high copy number plasmid DNA (200 to 20000 bp) from a 100 to 500 ml culture in less than 3 hours. The purified DNA can be used directly for DNA sequencing and restriction digestion without further manipulation and also can be used in vitro transcription reactions.

Supplier: Promega

The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution. The system is suitable for use in many molecular biology applications, including next-gen sequencing and other techniques where accurate DNA quantitation is important.

Supplier: Promega

The PureYield™ plasmid Maxiprep system is designed to isolate transfection quality plasmid DNA. The system provides a rapid method for purification up to 1 mg of plasmid DNA from a 250 ml bacterial culture.

Supplier: Promega

The MagaZorb DNA Kit provides an easy, fast and cost-effective technique for isolating PCR-quality DNA.

Supplier: Promega

The Wizard® Magnetic DNA Purification Kits for Food are used to isolate DNA from a variety of food samples like corn seeds, cornmeal, soybeans, soy flour and soy milk. Processed food, such as corn chips, chocolate and chocolate-containing foods, lecithin and vegetable oils can also be used with protocol variations.

Supplier: Promega

Advanced and streamlined DNA extraction for enhanced water quality testing.

Supplier: Promega

The Maxwell® 16 FFPE Plus LEV DNA Purification Kit is used to extract DNA rapidly from formalin-fixed, paraffin-embedded tissue samples using the Maxwell® 16 instrument. The kit is designed for optimal purification of DNA from one to ten 5 μm thin sections of FFPE tissue samples, maximising yield and eliminating the need to concentrate the extract prior to amplification.

Supplier: Promega

ReliaPrep™ RNA Miniprep systems provide a fast and simple technique for preparation of intact total RNA from cultured cells or tissue. The proprietary column/binding matrix efficiently captures RNA from very small amounts of input material, isolating RNA eluted in a minimal volume (less than 15 µl).

Supplier: Promega

The Wizard® PCR preps DNA purification system provides a simple, reliable way to purify double-stranded PCR amplified DNA. This system also can be used to purify DNA fragments from agarose gels. The DNA can be eluted in water or TE buffer, free of salts or macromolecular contaminants. Multiple PCR preps may be processed easily at one time with the Vac-Man® naboratory vacuum manifold.