You searched for: Nucleic Acid Purification Kits and Reagents

Supplier: Promega

SV 96 Total RNA Isolation System provides a high-throughput technique to prepare intact RNA from tissue and cultured cells. Total RNA can be purified from 96 samples in less than an hour without centrifugation.

Supplier: Promega

The E. coli S30 Extract System for Linear Templates allows successful transcription/translation of linear DNA templates. The investigator need only provide linear DNA containing a prokaryotic E. coli-like promoter (such as lacUV5, tac, λPL (con) and λ-PR). A ribosome binding site is required to direct the synthesis of proteins in vitro.

Supplier: Promega

The Maxwell® 16 Cell Low Elution Volume (LEV) DNA Purification Kit automatically extracts genomic DNA from sample types inherently low in cell number, such as amniotic fluid, cerebral spinal fluid (CSF) and cell culture supernatants. Purified genomic DNA can be easily obtained with samples containing less than 10000 cells. Used in combination with the Maxwell® 16 instrument.

Supplier: Promega

The resin-based Wizard® Plus Midipreps DNA Purification System provides a simple and reliable method for rapid isolation of plasmid DNA. Midipreps may be processed individually or in multiples with the Vac-Man® (20 sample capacity) or Vac-Man® Jr. (2 sample capacity) laboratory vacuum manifold. The purified plasmid can be used directly for automated fluorescent DNA sequencing and restriction digestion without further manipulation and also can be used for in vitro transcription reactions.

Supplier: Promega

The Wizard® SV 96 PCR clean up system is designed for high throughput purification of 100 bp to 10 kb PCR products from excess nucleotides, primers and primer dimers. The purified DNA can be used for automated fluorescent sequencing, cloning, labeling, restriction digestion or microarray analysis without further manipulation. The Wizard® SV 96 PCR clean-up system uses 96-well filtration without the need to disassemble the manifold.

Supplier: Promega

Maxwell® 16 Low Elution Volume (LEV) Blood DNA Kit is designed for optimal automated extraction of DNA from whole blood samples using the Maxwell® 16 instrument. Up to 300 μl of whole blood can be processed to yield up to 150 ng/μl. The Maxwell® 16 LEV Blood DNA Kit delivers buffy coat-like purity and concentration from whole blood, saving time and allowing automation. Used in combination with the Maxwell® 16 instrument.

Supplier: Promega

The Wizard® Plus SV Minipreps DNA purification system is a silica membrane based system, provides a simple and reliable method for rapid isolation of plasmid DNA. Using the system, plasmid DNA can be purified from 1 to 10ml of overnight E.coli culture. The purified plasmid DNA can be used directly for automated fluorescent BigDye® terminator DNA sequencing as well as for other standard molecular biology techniques without further manipulation and also in vitro transcription reactions.

Supplier: Promega

The MagneSil® Genomic, Fixed-Tissue System provides a fast, simple technique for extracting genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissue. After an overnight Proteinase K digestion, genomic DNA can be manually purified from FFPE thin tissue sections in less than an hour, and up to 12 samples can be processed at a time using the MagneSphere® technology magnetic separation stand (12-position).

Supplier: Promega

Wizard® Plus Megapreps DNA purification system provides a simple and rapid method for large scale purifications of plasmid DNA. This system requires only a centrifuge, a vacuum source and a vacuum manifold. The system yields greater than one milligram of high copy number plasmid DNA (200 to 20000 bp) from a 1000 ml culture in less than 3 hours. The purified DNA can be used directly for DNA sequencing and restriction digestion without further manipulation and also can be used for in vitro transcription reactions.

Supplier: Promega

The Wizard® Plus Maxipreps DNA purification system provides a simple and rapid resin based batch column method for purification of plasmid DNA that eliminates the need for cesium chloride:ethidium bromide gradient centrifugation. This system requires only a centrifuge, a vacuum source and a vacuum manifold. This system typically yields 300 μg to 1 mg of high copy number plasmid DNA (200 to 20000 bp) from a 100 to 500 ml culture in less than 3 hours. The purified DNA can be used directly for DNA sequencing and restriction digestion without further manipulation and also can be used in vitro transcription reactions.

Supplier: Promega

The Maxwell® 16 Blood DNA Purification Kit is designed for automated extraction of DNA from whole blood or buffy coat samples on the Maxwell® 16 instrument. The kit is optimised to process a wide range of volumes of fresh or stored human blood as well as animal blood samples. Up to 400 µl of whole blood can be processed to yield up to 15 µg of gDNA from human blood, depending on white blood cell (WBC) count.

Supplier: Promega

The Wizard® MagneSil® plasmid DNA purification system provides a simple and reliable method for the rapid isolation of plasmid DNA in a 96-well, high throughput format. The purified plasmid can be used directly for automated fluorescent sequencing, such as with BigDye® terminator sequencing chemistry, as well as for other standard molecular biology techniques including restriction enzyme digestion. The use of the MagneSil® paramagnetic particles for lysate clearing (BLUE) as well as DNA capture (RED) circumvents the need for centrifugation or vacuum manifolds, making the system ideal for full automation on a Beckman Coulter or Tecan instrument.

Supplier: Promega

The Wizard® SV 96 genomic DNA purification system provides a high-throughput, membrane based technique for consistent preparation of genomic DNA from cultured cells and tissue, including mouse tails. Amplifiable genomic DNA can be isolated from up to 5×10⁶ cells, 20 mg of tissue or up to 1,2 cm of a mouse tail tip without a centrifugation clearing step. With this system, genomic DNA is purified from cell lysates using 96-well vacuum filtration. Washing the bound DNA requires no disassembly of the manifold, and filtrate waste products are delivered directly to a vacuum trap, eliminating the need to empty waste collection trays.

Supplier: Promega

Automated high-throughput extraction of cell-free DNA from plasma, serum, urine and CSF.

Supplier: STEMCELL TECHNOLOGIES

For purification of RNA from cells.

Supplier: STEMCELL TECHNOLOGIES

Immunomagnetic extraction of total nucleic acid (DNA and RNA).

Supplier: STEMCELL TECHNOLOGIES

For extraction of DNA from agarose gels, or clean-up of PCR products and restriction enzyme digestions.

Supplier: STEMCELL TECHNOLOGIES

For purification of genomic DNA from cells or tissue.