You searched for: qPCR/RT-qPCR Enzymes and Kits

Supplier: Thermo Fisher Scientific

Luminaris Color HiGreen qPCR Master Mixes are universal ready to use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template and primers need to be added. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence-specific probes.

Supplier: AAT BIOQUEST

Real-time thermal cyclers require calibration to generate a spectral profile for the fluorescence signal.

Supplier: AAT BIOQUEST

FAM dye qPCR calibration solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ seven systems.

Supplier: AAT BIOQUEST

MP650 is designed to have identical spectra to those of Mustang Purple.

Supplier: Biotium

EvaGreen® Plus Dye has an improved signal-to-noise compared to original EvaGreen® dye, for higher sensitivity in DNA amplification applications.

Supplier: Biotium

A qPCR master mix containing Evagreen® qPCR dye and Cheetah™ Taq hotstart DNA polymerase, suitable for qPCR using a fast cycling protocol.

Supplier: Thermo Fisher Scientific

O sistema de RT-PCR Verso™ combina uma enzima de transcrição inversa, opções de purga melhoradas e tampões optimizados, para gerar um rendimento elevado e uma sequência completa de ADNc. Com várias opções de kit para as reacções RT-PCR de 1 e 2 passos, o kit Verso™ proporciona uma flexibilidade e uma sensibilidade excelentes num formato fácil de usar.

Supplier: BIOPREMIER

This Supreme RT-PCR detection kit HLVd/LCV/CanCV is designed for the detection of Hop Latent Viroid (HLVd), Lettuce Chlorotic Virus (LCV) and Cannabis Cryptic Virus (CanCV) in a single PCR reaction (TETRAPLEX).

Supplier: Thermo Fisher Scientific

ABsolute™ qPCR Mixes contain all the components necessary to perform quantitative PCR, with the exception of template and primers. Versions are available for use with probes or with SYBR® Green I included, and with or without ROX or Fluorescein passive reference dyes.

Supplier: Thermo Fisher Scientific

As misturas para capilares para qPCR Absolute™ contêm um tampão de reacção optimizado, que foi especificamente concebido para obtenção de máximos em termos de sensibilidade, especificidade e consistência de instrumentos em tempo real que utilizam capilares de vidro, tais como o Roche LightCycler®. As misturas de 2X contêm todos os componentes necessários para efectuar a PCR quantitativa, à excepção de modelos e sondas/primers.

Supplier: BIOPREMIER

SUPREME RT-PCR detection kit HLVd is designed for the detection of Hop Latent Viroid (HLVd) in a single PCR reaction. The kit is suitable for use in real time instruments with FAM and Cy5 detection channels.

Supplier: PRIMERDESIGN

The oasig freeze drying process stabilises all of the active components allowing these reagents to be shipped and stored at room temperature. This hugely simplifies the logistics of purchasing, shipping and using the technology. Whether you are in a sophisticated laboratory in Texas or a mobile field hospital in Timbuktu we can supply complete qPCR kit and reagent packages to your door quickly and cheaply via standard shipping methods without the need for dry ice or a cold chain of any sort.
Freeze dried qPCR reagents stored and shipped at ambient temperatures:

Supplier: PRIMERDESIGN

This genesig® Easy qPCR detection kit targets the invasion protein (invA) gene from Salmonella spp. The kit is designed for the in vitro quantification of Salmonella spp genomes and to have a broad detection profile.

Supplier: Thermo Fisher Scientific

A mistura ABsolute Blue™ master mix incorpora um corante azul inerte para melhorar significativamente o contraste entre o reagente e o plástico, tornando a verificação da dispensação do master mix mais rápida e extremamente fácil. As suas aplicações incluem expressão genética, validação de siRNA, tipagem e genotipagem de SNP.

Supplier: Thermo Fisher Scientific

O sistema de qRT-PCR Verso™ combina a enzima de RT Verso™ com a master mix Blue qPCR ABsolute™, para proporcionar a maior comodidade e a maior versatilidade possíveis. Disponíveis no formato de 1 ou 2 etapas, bem como em vários tamanhos de embalagem, para satisfazer todos os requisitos das aplicações e de débito.

Supplier: Thermo Fisher Scientific

As misturas para qPCR ABsolute™ com baixo teor de ROX são optimizadas para a utilização com instrumentos em tempo real que têm a capacidade de detectar, com mais sensibilidade, corantes com comprimento de onda elevado, tal como o ABI 7500. O baixo teor de ROX facilita a normalização em toda a placa, ao mesmo tempo que mantém a sensibilidade do ensaio. As misturas contêm todos os componentes necessários para efectuar a PCR quantitativa, à excepção de modelos e sondas/iniciadores. A mistura para qPCR de 2X contém um tampão de reacção patenteado que proporciona sensibilidade, especificidade e reprodutibilidade em elevado grau.

Supplier: Promega

Use Carboxy-X-Rhodamine (CXR) Reference Dye (identical to ROX™ dye) with instruments that require a higher level of reference dye that than in the GoTaq® qPCR Master Mix. Component of GoTaq® qPCR and RT-qPCR Systems.

Supplier: Promega

The GoTaq® 2-Step RT-qPCR system facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components such as GoScript™ reverse transcription system and GoTaq® qPCR master mix. It includes an optimised reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

Supplier: Promega

The GoTaq® qPCR Master Mix is optimised for fast and reproducible quantitative PCR assays using a double-stranded DNA binding dye. The master mix is provided as a ready to use, stabilised 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl₂, dNTPs, BRYT Green® dye and a proprietary reaction buffer.

Supplier: Promega

The GoTaq® 2-Step RT-qPCR system facilitates detection and relative quantification of RNA expression levels via a two-step RT-qPCR method using integrated components such as GoScript™ reverse transcription system and GoTaq® qPCR master mix. It includes an optimised reaction buffer and M-MLV reverse transcriptase designed to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification. The cDNA product can be added directly to downstream qPCR amplification reactions.

Supplier: Promega

Reliable detection of Legionella pneumophila in water supplies is important to prevent occurrence of Legionnaires’ disease.

Supplier: Promega

The GoTaq® Probe qPCR Master Mix is optimised for fast and reproducible quantitative PCR assays in the hydrolysis probe detection format. The master mix is provided as a ready to use, stabilised 2X formulation and contains GoTaq® Hot Start Polymerase, MgCl₂, dNTPs and a proprietary reaction buffer.

Supplier: Promega

The GoTaq® 1-Step RT-qPCR system enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR master mix in a single-step real-time amplification reaction. It combines optimised quantities of GoScript™ Reverse Transcriptase, RNasin® Plus RNase inhibitor and additives to enhance single-step reactions.

Supplier: Promega

The Viability PCR Reagent System uses a proprietary molecule that irreversibly modifies nucleic acid so that it cannot be amplified. This molecule is cell- and capsid-impermeable, enabling selective detection of nucleic acid from only live cells or intact viruses.

Supplier: Promega

The GoTaq® 1-Step RT-qPCR system enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and GoTaq® qPCR master mix in a single-step real-time amplification reaction. It combines optimised quantities of GoScript™ Reverse Transcriptase, RNasin® Plus RNase inhibitor and additives to enhance single-step reactions.

Supplier: STEMCELL TECHNOLOGIES

For probe-based assays and arrays.

Supplier: STEMCELL TECHNOLOGIES

qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells.