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You searched for: Cell Culture Supplements and Additives

Cell Culture Supplements and Additives

The tissue culture media supplements and additives compensate for characteristics the base medium lacks in providing maximum cell growth conditions. Incorporating the substances during development brings stability to the cultivated cell environments for maximum activity control. The stabilizing liquids added will support cell productivity, isolation, and microorganism identification. With a variety of tissue culture supplements and additives concentrations offered, a substance possessing the correct compatibility even with sensitive mediums is guaranteed.

VWR® Egg Yolk Tellurite Emulsion

VWR® Egg Yolk Tellurite Emulsion

Supplier: VWR Chemicals

Egg yolk tellurite emulsion, VWR®

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VWR®, Yeast extract

VWR®, Yeast extract

Supplier: VWR Chemicals

Yeast extract is the water soluble portion of autolysed yeast containing a source of Vitamin B complex.

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VWR®, Protease inhibitor cocktails, general use

VWR®, Protease inhibitor cocktails, general use

Supplier: VWR Chemicals

Contains a mixture of protease inhibitors: AEBSF, Aprotinin, E-64, Bestatin and Leupeptin. Each vial can be reconstituted in 1 ml deionised water to form a 100X solution.

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VWR® Egg Yolk Emulsion, Sterile

VWR® Egg Yolk Emulsion, Sterile

Supplier: VWR Chemicals

Egg yolk emulsion, sterile, VWR®

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VWR® Protease Inhibitor Cocktail, Plant

VWR® Protease Inhibitor Cocktail, Plant

Supplier: VWR Chemicals

Contains a mix of protease inhibitors: AEBSF, E-64, Bestatin, Pepstatin, Leupeptin and 1, 10-Phenanthroline. Each vial can be reconstituted in 1 ml dimethyl sulfoxide to form a 100X solution.

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VWR®, Trypsin-EDTA solutions

VWR®, Trypsin-EDTA solutions

Supplier: VWR Chemicals

VWR® Trypsin-EDTA solutions are used in cell culture to remove adherent cells from a culture surface. Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to separate cells from their substrate is dependent primarily on the cell type and the age of the culture. EDTA, a chelating agent, enhances enzymatic activity by removing calcium and magnesium ions. These ions obscure the peptide bonds on which trypsin acts as well as enhancing cell to cell adhesion.

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HyClone Cell Boost™ 7b supplement

HyClone Cell Boost™ 7b supplement

Supplier: HyClone products (Cytiva)

Supplements cell culture with concentrated amino acids. Manufactured to meet cGMP manufacturing standards and QC specifications.

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Protease inhibitor cocktail

Supplier: ENZO LIFE SCIENCES

Protease inhibitor cocktail

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Serum-free growth medium, defined, HemaPro

Serum-free growth medium, defined, HemaPro

Supplier: PROTIDE PHARMACEUTICALS

Serum supplement for the culturing of human progenitor cells. Effective in studying cancer stem cells, iPSC, and mesenchymal stem cells.

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HyClone Cell Boost™ 3 supplement

HyClone Cell Boost™ 3 supplement

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids, vitamins, glucose, and trace elements and manufactured to meet cGMP manufacturing standards and QC specifications.

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HyClone Cell Boost™ 4 supplement

HyClone Cell Boost™ 4 supplement

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids, vitamins, glucose, trace elements, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.

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Yeast extract, molecular biology grade

Supplier: PanReac AppliChem

Water-soluble part of autolysed yeast. Rich of ammonia and vitamins, especially of water-soluble vitamin B-complexes; suitable for addition to media.

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Autophagy library, Screen-Well®

Supplier: ENZO LIFE SCIENCES

Structurally and mechanistically different compounds for studying the roles of pro- and anti-autophagic molecules in cells and in vitro applications.

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Versene

Versene

Supplier: Biowest

Versene can be used instead of trypsin. It is a chelating agent that disperses the cells by cutting the cytoplasmic bridges between them. Versene has the advantage of being able to withstand autoclave temperature, which is a guarantee of sterility. In addition, it is less aggressive cells than trypsin, which is useful for studies on cell growth.

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Stemsol 99,99% DMSO USP, Ph. Eur.

Stemsol 99,99% DMSO USP, Ph. Eur.

Supplier: PROTIDE PHARMACEUTICALS

Material grade - USP, Ph. Eur.

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Cryopreservation solutions, DMSO-free, pZerve™

Cryopreservation solutions, DMSO-free, pZerve™

Supplier: PROTIDE PHARMACEUTICALS

A unique cryopreservation solution that does not contain DMSO, foetal bovine serum, or animal protein. pZerve™ is ready to use and does not require any dilution or further processing.

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MEM non essential amino acids 100X

MEM non essential amino acids 100X

Supplier: Biowest

Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.

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TCH defined serum-free growth medium

TCH defined serum-free growth medium

Supplier: PROTIDE PHARMACEUTICALS

Serum replacement used primarily for human cells and the production of cell secreted proteins. TCH™ is also effective in culturing other mammalian cells without the use of animal proteins. Contains only human proteins (i.e., human serum albumin, human transferrin, recombinant insulin), which are heat treated.

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Trypsin Protease, HyClone™

Trypsin Protease, HyClone™

Supplier: HyClone products (Cytiva)

Dissociation reagents, such as trypsin, are used to detach and disaggregate adherent cells from the surface or substrate to which they are attached, as well as for dissociating tissues for primary cell culture.

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GrowDex-T® Hydrogel

GrowDex-T® Hydrogel

Supplier: UPM Biomedicals

GrowDex®-T Hydrogel is a bio-friendly hydrogel extracted from birch and sourced from sustainable and responsibly managed forests.

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Phenol red, sodium salt

Supplier: Biowest

Phenol red is used as a pH indicator in cell culture. Its colour exhibits a gradual transition from yellow to red over the pH range 6,8 to 8,2. Above pH 8,2, phenol red turns a bright pink (fuchsia) colour.

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Phosphatase inhibitor cocktails, PhosphataseArrest™

Supplier: G-Biosciences

The PhosphataseArrest™ phosphatase inhibitor cocktails are ready to use 100X solutions that are simply added to either extraction buffers or samples.

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L-Glutamine, HyClone™

Supplier: HyClone products (Cytiva)

HyClone™ L-glutamine is used as a supplement in serum- and protein-free media. It supports the growth of cells that have high energy demands and synthesise large amounts of proteins and nucleic acids.

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Protease inhibitor cocktail, Recom ProteaseArrest™

Protease inhibitor cocktail, Recom ProteaseArrest™

Supplier: G-Biosciences

Recom ProteaseArrest™ is a broad range, bacterial, 100X concentrated, ready to use protease inhibitor cocktail. Recom ProteaseArrest™ offers greater protection for recombinant proteins expressed and purified from bacteria. Recom ProteaseArrest™ inhibits bacterial serine, cysteine, metallo- and other bacterial specific proteases, including aminopeptidases and aspartic proteases.

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Cell dissociation reagents, HyrTryp™

Cell dissociation reagents, HyrTryp™

Supplier: HyClone products (Cytiva)

With HyrTryp™ cell dissociation reagent, an animal-derived component-free (ADCF) recombinant alternative to porcine trypsin, you can dissociate and harvest cells with confidence. Simply substitute it in your existing protocol.

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Protease inhibitor cocktail, FOCUS™ ProteaseArrest™

Protease inhibitor cocktail, FOCUS™ ProteaseArrest™

Supplier: G-Biosciences

A ready to use, 100X concentrated, broad range protease inhibitor cocktail that is fully compatible with 2D electrophoresis and subsequent mass spectrometry.

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Supplement Kit for Skeletal Muscle Cell Growth Medium, FCS

Supplier: PROVITRO

The Provitro skeletal muscle cell growth medium is a sterile liquid culture medium for culturing human skeletal muscle cells (HSKMC). The medium is delivered as a basal medium and is suitable for culturing Provitro HSKMC after adding the supplement kit components. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx. 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.

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M9 Minimal Salts (Maniatis Formulation), Powder, MP Biomedicals

M9 Minimal Salts (Maniatis Formulation), Powder, MP Biomedicals

Supplier: MP Biomedicals

M9 Minimal medium salts are suitable for the preparation of M9 Minimal Medium for use in propagation of E.coli and plasmid amplification.

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Cryopreservation solution, DMSO-free, Cellvation™

Cryopreservation solution, DMSO-free, Cellvation™

Supplier: PROTIDE PHARMACEUTICALS

A unique cryopreservation solution that does not contain DMSO or foetal bovine serum. Cellvation™ is convenient to use and provides optimal recovery. Cells can be exposed to Cellvation™ for longer periods of time before the freezing process is initiated. Cellvation™ is ready to use and can be used for cells cultured in serum-free medium or with serum-containing medium.

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HyClone Cell Boost™ 1 supplement

HyClone Cell Boost™ 1 supplement

Supplier: HyClone products (Cytiva)

Supplements cell culture with amino acids, vitamins, and glucose and manufactured to meet cGMP manufacturing standards and QC specifications.

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