Enzymes accelerate, or catalyze, chemical reactions, and they are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Choose specific enzymes for cleaving bonds, removing genomic DNA from RNA preparations, for producing fragments of proteins, or for use in ion exchange chromatography. Enzymes are used in the chemical industry and other industrial applications when extremely specific catalysts are required.

SacI (from Streptomyces achromogenes)

Supplier: Promega

SacI recognises the same DNA sequence but cleaves at a different nucleotide than its neoschizomer, EcoICRI. SacI recognises G AGCT^▾C and C_▴TCGA G.

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Ribonuclease H

Supplier: Promega

Ribonuclease H (RNase H) is an endonuclease that specifically hydrolyses the phosphodiester bonds of RNA hybridised to DNA to produce 3´-OH and 5´-P-terminated products. It will not degrade single-stranded nucleic acids, double-stranded DNA or double-stranded RNA. This endonuclease can be used in applications such as microarrays.

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Trypsin

Supplier: Promega

Immobilised trypsin provides a fast and convenient method for digesting a range of concentrations of purified protein or complex protein mixtures. Digested peptides are easily separated from the immobilised trypsin as they flow through the spin column into the collection tube. Immobilised trypsin is easily removed from the peptide solution because the trypsin does not pass though the column frit.

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DNase

Supplier: Promega

RQ1 RNase-Free DNase is a preparation of deoxyribonuclease (DNase) I that degrades single-stranded or double-stranded DNA to produce 3´-hydroxyl oligonucleotides.

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ProTEV Plus

Supplier: Promega

ProTEV Plus is an improved 48 kDa version of the Nla protease from tobacco etch virus (TEV) that has been engineered to be more stable than native TEV protease for prolonged enzymatic activity.

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Trypsin

Supplier: Promega

Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues. The stringent specificity of trypsin is essential for protein identification.

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Proteinase K

Supplier: Promega

Proteinase K cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids and is useful for general digestion of protein in biological samples.

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Cellulase

Supplier: Merck

Cellulase, enzyme blend contains cellulases, ß-glucosidases, and hemicellulase, for the application of degrading cellulose to fermentable sugars.

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Bacillus polymyxa Dispase II (from Bacillus polymyxa)

Supplier: Merck

The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7,4, 150 mM NaCl at 10 mg/ml.

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Recombinant SOLu-trypsin (from P. pastoris)

Supplier: Merck

SOLu-Trypsin is an advanced proteomics grade enzyme that is solution stable for mass spectrometry. Recombinant, expressed in Pichia pastoris.

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exo-1,3-β-D-Glucanase + β-Glucosidase

Supplier: MEGAZYME

High purity exo-1,3-β-D-Glucanase (Trichoderma sp.) + β-Glucosidase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

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Acetylcholinesterase

Supplier: Merck

Acetylcholinesterase from Electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kDa each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.

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Ascorbate oxidase (from Cucumis sp.)

Supplier: Merck

Ascorbate Oxidase is an enzyme belonging to the family of oxidoreductases that catalyses the reaction: Ascorbic acid + ½ O₂ ascorbate oxidase → Dehydroascorbic acid + H₂O.

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α-Amylase (from Aspergillus oryzae)

Supplier: Merck

Aspergillus oryzae α-amylase enzyme catalyzes the hydrolysis of the α-1,4 glycosidic bonds in soluble starches and related subsrates. These substrates are broken down to release short oligosaccharides and α-limit dextrins. Also act as control enzyme in agar plate-based and carboxymethylcellulose-based clearing assays to screen cellulase activity.

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Trichoderma endo-1,3-beta-D-glucanase (from Trichoderma sp.)

Supplier: MEGAZYME

endo-1,3-β-D-Glucanase is isolated from Trichoderma species. These are used in carbohydrate and biofuels research, biochemical enzyme assays and in vitro diagnostic analysis.

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DENARASE® High Salt

Supplier: C LECTA

DENARASE High Salt is an engineered version of the wild-type Serratia marcescens endonuclease, which is commonly used for DNA removal applications in bioprocessing. Compared to the wild-type DENARASE, a few amino acids were substituted to provide the enzyme with an improved salt tolerance without losing its specificity for nucleic acids.