You searched for: Enzymes

Supplier: MP Biomedicals

Dissolve at a concentration of 1 mg/ml. Dilute further to a concentration of 20 to 60 U/ml immediately before the assay.

Supplier: MP Biomedicals

Neuraminidase of non-pathogenic streptococci, is prepared from the culture filtrate by Ammonium Sulfate precipitation and Ion-exchange chromatography.

Supplier: MP Biomedicals

Dissolves readily at 2 mg/ml in analytical grade water to give a clear colorless solution.

Supplier: MP Biomedicals

Zymolyase 100T is used to lyse living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. It may also be used for glucan hydrolysis.

Supplier: MP Biomedicals

DIASTASE from malt, 1X N.F. stable, 60 mesh powder. Ready for CE IVD labeling of clinical applications.

Supplier: MP Biomedicals

Cellulase Y-C removes cell walls from plant tissues in shorter incubation period without loss of biological activity of the materials.

Supplier: MP Biomedicals

Acid phosphatase (APase) non-specifically catalyses the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell.

Supplier: MP Biomedicals

Protease isolated from S. aureus, strain V8, this protease exhibits cleavage of peptide bonds on the COOH-terminal side of either aspartate or glutamate residues.

Supplier: MP Biomedicals

Alkaline phosphatase is a phosphomonoesterase, used to remove 5′-phosphate groups from nucleic acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulations.

Supplier: MP Biomedicals

Pectolyase Y-23 contains two types of pectinase such as endopolygalacturonase and endo-pectin lyase in high activity. It includes maceration stimulating factor which stimulates tissue maceration by both pectinases.

Supplier: MP Biomedicals

Horseradish peroxidase (HRP) is isolated from horseradish roots and belongs to the ferroprotoporphyrin group of peroxidases. It is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme.

Supplier: MP Biomedicals

Catalase, fungal suspension from from Aspergillus niger is long-acting, extremely stable form of catalase that is active over a wide pH range. Composed of four protein subunits, each containing a heme group bound to its active site.

Supplier: MP Biomedicals

Inhibitors: p-Chloromercuribenzoate, iodoacetamide, heavy metal ions (Zn²⁺, Fe²⁺, Zn²⁺, Cd²⁺, Cu²⁺, Pb²⁺, Ag⁺, Hg²⁺), ionic Detergents (SDS, DAC, etc.). Contaminants: The preparation is practically free from other glycosidases (a-galactosidase, a-,b-glucosidase, a-,b-mannosidase, etc.) and proteinase. Principle: o-Nitrophenyl-b-D-galactopyranoside (ONPG) b-galactosidase > o-Nitrophenol (ONP) + D-Galactose. The appearance of o-nitrophenol is measured at 410 nm by spectrophotemetry. Thermal Stability: Below 50 °C (pH 7,3; 15 min) (Lit.), Optimum Temperature: 50 to 55 °C (Lit.).

Supplier: MP Biomedicals

Elastase is prepared from porcine pancreas. It is lyophilised and water soluble. Elastase is chromatographically prepared by the method of Narayanan and Anwar. In the method, 2 times crystalline elastase is adsorbed on a column of DEAE-Sephadex A50 to separate elastase and non-specific protein components. The elastase component is further purified by chromatography on a column of CM-Cellulose using a sodium chloride gradient to elute the elastase. The latter is dialysed until chloride-free and then lyophilised. During its preparation the elastase is held below a pH of 5,5 for greater than 24 hours. Two times crystallised from the euglobin fraction of porcine pancreas by the method of Lewis et al. Does not contain trypsin or chymotrypsin.

Supplier: Promega

IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). IdeZ Protease is an immunoglobulin-degrading enzyme from Streptococcus equi subspecies zooepidemicus.

Supplier: Promega

S1 Nuclease is an endonuclease that degrades ssDNA and RNA. The enzyme is used to remove protruding single-stranded termini from double-stranded DNA, for selective cleavage of single-stranded DNA and for mapping RNA transcripts.

Supplier: Promega

RNase ONE™ Ribonuclease is a 27 kDa periplasmic enzyme from E. coli that catalyses the degradation of RNA to cyclic nucleotide monophosphate (NMP) intermediates. Slower hydrolysis further catalyses the degradation of these intermediates to 3´-NMPs. RNase ONE™ Ribonuclease may be used to remove RNA from DNA preparations, for mismatch detection, RNase protection assays and mapping, or quantitation of RNA by selective cleavage of single-stranded regions.

Supplier: Promega

Alkaline Phosphatase, Calf Intestinal (CIAP), catalyses the hydrolysis of 5’-phosphate groups from nucleic acids and nucleotide triphosphates. This enzyme is used to prevent recircularisation and religation of linearised cloning vector DNA by removing phosphate groups from both 5’-termini and may also be used for the dephosphorylation of 5’ phosphorylated ends of DNA or RNA for subsequent labelling with [32P]ATP and T4 Polynucleotide Kinase. CIAP is active on 5’ overhangs, 5’ recessed and blunt ends.

Supplier: Promega

A highly-purified serine endopeptidase derived from bovine pancreas that preferentially cleaves at the carboxyl side of aromatic amino acids Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal at pH of 7.0–9.0. This sequencing grade enzyme can be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

Supplier: Promega

PNGase F is a recombinant glycosidase cloned from Elizabethkingia miricola and overexpressed in E. coli.

Supplier: Promega

Terminal deoxynucleotidyl transferase, recombinant, catalyses the repetitive addition of mononucleotides to the terminal 3´-OH of a DNA initiator accompanied by the release of inorganic phosphate.

Supplier: Promega

Bovine alpha-Chymotrypsin (from Pancreas)

Supplier: Promega

Exonuclease III catalyses the stepwise removal of mononucleotides from dsDNA starting from a 3'-OH at nicks, blunt ends, recessed ends and 3'-overhangs of less than 4 bases, yielding nucleoside 5'-phosphates.

Supplier: Promega

Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues. The stringent specificity of trypsin is essential for protein identification.

Supplier: Promega

SacI recognises the same DNA sequence but cleaves at a different nucleotide than its neoschizomer, EcoICRI. SacI recognises G AGCT^▾C and C_▴TCGA G.

Supplier: Promega

Trypsin is a serine protease that specifically cleaves at the carboxylic side of lysine and arginine residues. The stringent specificity of trypsin is essential for protein identification.

Supplier: Promega

Proteinase K cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids and is useful for general digestion of protein in biological samples.

Supplier: Promega

T4 PNK catalyzes the transfer of the gamma-phosphate from ATP to the 5'-terminus of polynucleotides or to mononucleotides bearing a 5'-hydroxyl group.

Supplier: Promega

Factor Xa protease preferentially cleaves after the arginine residue in the amino acid sequence Ile-Glu-Gly-Arg.

Supplier: Promega

BglI recognises the defined internal six bases of the SfiI recognition site and also cuts at the same position. It recognises the GCCN NNN^˅NGGC and CGGN_˄NNN NCCG.