Sanger Sequencing Reagents
Sequencing reagents facilitate the processes when determining the precise order of nucleotides within DNA strands through sorting the fragments based on size. Enzymes are optimized to work together for high-efficiency removal of unincorporated primers and nucleotides. These reagents produce exceptional sequencing results from difficult templates, such as those with high GC content and secondary structures. Essential for basic and applied research procedures, sequencing reagents allow researchers to discover genotypes and phenotypes of specimen.
Fragment selection kits, Axygen® AxyPrep™ FragmentSelect-I Kit
Supplier: Corning
The AxyPrep™ FragmentSelect Kits utilise a paramagnetic bead technology for quick high throughput optimised DNA size selection suitable for various next generation sequencing platforms. The AxyPrep™ FragmentSelect-I kit is optimised for Illumina next generation sequencing platforms and Life Technologies (SOLiD™) fragment size selection needs simplifying the library construction workflow.
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MagaDye™ 561 ddATP
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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MagaDye™ 588 ddTTP
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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2-Aminoethoxypropargyl ddGTP (1 µmole)
Supplier: AAT Bioquest
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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MagaDye™ 535 ddGTP
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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ddGTP [2',3'-Dideoxyguanosine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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ddTTP [2',3'-Dideoxythymidine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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MagaDye™ 613 ddCTP
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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2-Aminoethoxypropargyl ddCTP (1 µmole)
Supplier: AAT Bioquest
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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ddCTP [2',3'-Dideoxycytidine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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2-Aminoethoxypropargyl ddATP (1 µmole)
Supplier: AAT Bioquest
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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ddATP [2',3'-Dideoxyadenosine-5'-triphosphate] *10 mM in ddH₂O* (1 µmole)
Supplier: AAT Bioquest
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase.
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2-Aminoethoxypropargyl ddTTP (1 µmole)
Supplier: AAT Bioquest
Sanger method is one of the most reliable and earliest DNA sequencing methods.
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Lysine-C™, sequencing grade
Supplier: G-Biosciences
SG-Lysine-C™ endopeptidase, from Lysobacter enzymogenes, is a serine protease highly specific in cleaving peptide bonds at the carboxy side of lysine. Highly purified preparations of SG-Lysine-C™ are chemically modified making the enzyme resistant to autolysis and stabilising its enzymatic activity.
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Arginine-C™, sequencing grade
Supplier: G-Biosciences
SG-Arginine-C™ endopeptidase (Clostripain, from C. histolyticum) specifically hydrolyses the carboxy peptide bond of arginine. SG-Arginine-C™ has been modified chemically by a propriety process to render the enzyme resistant to autolysis and stabilise enzymatic activity. In addition, as a sulfhydryl enzyme, SG-Arginine-C™ is susceptible to inactivation by oxidation and as a result requires reducing agents for protection. The enzyme also requires calcium ion for maximal activity. A special reconstitution buffer is supplied, which contains reducing agents and activators to maintain enzyme activity.
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Chymotrypsin™, sequencing grade
Supplier: G-Biosciences
SG-Chymotrypsin™ is a serine endopeptidase, which predominantly cleaves peptide bonds on the carboxy side of tyrosine, phenylalanine and tryptophan. In addition, chymotrypsin has a low catalytic activity against the carboxy side of leucine, methionine, alanine, aspartic and glutamic acids. It is therefore recommended to always use the shortest digestion time possible.
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Catálogo #: 83639.320
Fornecedor: VWR Chemicals
Catálogo #: 20864.320
Fornecedor: VWR Chemicals
Catálogo #: 83640.320
Fornecedor: VWR Chemicals
Catálogo #: 31770.294
Fornecedor: VWR Chemicals
Catálogo #: 1.00030.2500
Fornecedor: Merck
Catálogo #: 83645.320
Fornecedor: VWR Chemicals