You searched for: Cell Culture Supplements and Additives
The tissue culture media supplements and additives compensate for characteristics the base medium lacks in providing maximum cell growth conditions. Incorporating the substances during development brings stability to the cultivated cell environments for maximum activity control. The stabilizing liquids added will support cell productivity, isolation, and microorganism identification. With a variety of tissue culture supplements and additives concentrations offered, a substance possessing the correct compatibility even with sensitive mediums is guaranteed.
Autophagy library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
Structurally and mechanistically different compounds for studying the roles of pro- and anti-autophagic molecules in cells and in vitro applications.
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Yeast extract, molecular biology grade
Supplier: PanReac AppliChem
Water-soluble part of autolysed yeast. Rich of ammonia and vitamins, especially of water-soluble vitamin B-complexes; suitable for addition to media.
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Versene
Supplier: Biowest
Versene can be used instead of trypsin. It is a chelating agent that disperses the cells by cutting the cytoplasmic bridges between them. Versene has the advantage of being able to withstand autoclave temperature, which is a guarantee of sterility. In addition, it is less aggressive cells than trypsin, which is useful for studies on cell growth.
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Cryopreservation solutions, DMSO-free, pZerve™
Supplier: PROTIDE PHARMACEUTICALS
A unique cryopreservation solution that does not contain DMSO, foetal bovine serum, or animal protein. pZerve™ is ready to use and does not require any dilution or further processing.
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MEM non essential amino acids 100X
Supplier: Biowest
Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.
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TCH defined serum-free growth medium
Supplier: PROTIDE PHARMACEUTICALS
Serum replacement used primarily for human cells and the production of cell secreted proteins. TCH™ is also effective in culturing other mammalian cells without the use of animal proteins. Contains only human proteins (i.e., human serum albumin, human transferrin, recombinant insulin), which are heat treated.
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Protease inhibitor cocktail, Recom ProteaseArrest™
Supplier: G-Biosciences
Recom ProteaseArrest™ is a broad range, bacterial, 100X concentrated, ready to use protease inhibitor cocktail. Recom ProteaseArrest™ offers greater protection for recombinant proteins expressed and purified from bacteria. Recom ProteaseArrest™ inhibits bacterial serine, cysteine, metallo- and other bacterial specific proteases, including aminopeptidases and aspartic proteases.
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Protease inhibitor cocktails, ProteCEASE™
Supplier: G-Biosciences
ProteCEASE™ is a dry format version of ProteaseArrest™ for large scale preparative applications and for those users who prefer reconstitution prior to use. ProteCEASE™ is a superior general protease inhibitor cocktail that is suitable for purification from mammalian, plant, bacteria and yeast samples. The cocktail contains both irreversible and reversible protease inhibitors to inhibit serine, cysteine and other proteases. EDTA is an optional component for inhibiting metalloproteases.
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Ionotropic glutamatergic ligand library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® ionotropic glutamatergic ligands plate contains 60 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The ionotropic glutamatergic ligands plate is 1 plate of the larger neurotransmitter library which contains over 680 ligands.
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VWR® Egg Yolk Emulsion, Sterile
Supplier: VWR Chemicals
Egg yolk emulsion, sterile, VWR®
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MEM vitamins 100X concentrate
Supplier: Biowest
Concentrated solutions of amino acids, vitamins or non essential amino acids can be added to a basal medium to enrich it and the resulting medium used as a classical MEM or BME. The final concentration of the MEM or BME must be 1X.
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Epigenetics library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® epigenetics library contains 43 compounds with defined activity against enzymes which carry out epigenetic modification of lysine. It also includes DNA methylation inhibitors. Compounds are dissolved in DMSO at 10 mM and aliquoted into deep-well plates at 100 µl or 500 µl per well. The library is a useful tool for chemical genomics, assay development and other pharmacological applications. The library contains inhibitors of these important enzymes: HDACs, SIRTs, Lysine demethylases, HATs, Histone methyl transferases, DNA methyltransferases as well as SIRT activators. A variety of structurally and mechanistically different compound classes are included.
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HyClone Cell Boost™ 1 supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with amino acids, vitamins, and glucose and manufactured to meet cGMP manufacturing standards and QC specifications.
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HyClone Cell Boost™ 2 Supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with amino acids and glucose and manufactured to meet cGMP manufacturing standards and QC specifications.
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HyClone Cell Boost™ 5 supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with lipids, amino acids, vitamins, and growth factors. Manufactured to meet cGMP manufacturing standards and QC specifications.
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HyClone Cell Boost™ 7a supplement
Supplier: HyClone products (Cytiva)
Supplements cell culture with amino acids, vitamins, salts, trace elements, and glucose. Manufactured to meet cGMP manufacturing standards and QC specifications.
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D-Glucose Monohydrate (Dextrose), cell culture tested
Supplier: Biowest
D-glucose monohydrate (Dextrose) is a common natural sugar involved in processes such as energy production, glycosylation, and formation of glycans that provide structure to cells. It is involved in a detrimental process in cells called glycation. It is used as a supplement for cell culture and in numerous cellular processes.
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Cell detachment solution, Accutase
Supplier: Biowest
Accustase is a ready to use cell detachment solution of proteolytic and collagenolytic enzymes. Accutase is a direct gentle replacement for trypsin solution. It can also be used on suspension cells to reduce their agglutination in order to ease counting.
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FDA approved drug library V2, Screen-Well®
Supplier: ENZO LIFE SCIENCES
Accelerates drug optimisation with a unique collection of compounds of known safety and bioavailability for diverse targets.
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Protease inhibitor cocktail, Yeast/Fungal ProteaseArrest™
Supplier: G-Biosciences
A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Yeast/ Fungal ProteaseArrest™ inhibits yeast and fungal serine, cysteine and metalloproteases.
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Protease inhibitor cocktail, Mammalian ProteaseArrest™
Supplier: G-Biosciences
A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Mammalian ProteaseArrest™ inhibits mammalian serine, cysteine and other mammalian specific proteases including aminopeptidases, trypsin-like and aspartic proteases.
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Cryopreservation solutions, defined, 2-8CELLsius + DMSO
Supplier: PROTIDE PHARMACEUTICALS
When combined with DMSO, 2-8CELLsius™ can be utilised as a cryopreservation solution. 2-8CELLsius™ + DMSO is a cytoprotective, protein-free solution, with bio-stabilisers that support high post thaw cell viability. Optimal recovery is achieved through a novel approach to cell physiology at ultra-low temperatures.
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Supplement Kit for MVEC Growth Medium, Advanced, FCS
Supplier: PROVITRO
The supplement kit for microvascular endothelial cell growth medium, advanced is suitable for culturing Provitro human microvascular endothelial cells after adding the supplement kit components to 500 ml basal medium. The formulation is optimised for initial seeding of 4000 cells/cm² up to confluence (approx 90%). Feeder-layer, matrix substrates or other substances are not necessary. Due to the possibility of reduced proliferative activity we recommend to use the antibiotic supplement for freshly isolated cells only.
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Short-term storage solutions, 2-8CELLsius
Supplier: PROTIDE PHARMACEUTICALS
Ready to use, balanced, cytoprotective, protein-free solution with bio-stabilisers for short-term storage, regenerative medicine, stem cell processing and transportation of biological material. Short-term storage can be achieved at 2 to 8 °C or at ambient temperature for 24 to 48 hours. Does not contain any growth factors, hormones, or antibiotics. 2-8CELLsius™ can also be utilised as a cell washing solution.
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Dopaminergic ligand library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® dopaminergic ligands plate contains 80 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The dopaminergic ligands plate is 1 plate of the larger neurotransmitter library which contains over 650 ligands.
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Serotonergic ligand library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® serotonergic ligands plate contains 79 ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The serotonergic ligands plate is 1 plate of the larger neurotransmitter library which contains over 680 ligands.
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Purinergic ligand library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® purinergic and adenosine ligands plate contains 72 CNS receptor ligands including endogenous neurotransmitters, agonists, antagonists, and marketed drugs. The library is ideal for screening or identifying recombinant orphan G protein coupled receptors, target validation, secondary screening, assay development, and other pharmacological applications. The purinergic and adenosine ligands plate is 1 plate of the larger neurotransmitter library which contains over 650 ligands.
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Cardiotoxicity library, Screen-Well®
Supplier: ENZO LIFE SCIENCES
The Screen-Well® cardiotoxicity library is a focused collection of 130 compounds with defined and diverse cardiotoxicity, including ion channel blockage, mitochondrial toxicity, arrhythmia, fibrosis, and many more. A variety of structurally and mechanistically different compound classes are included, as well as nontoxic controls. The compounds are dissolved in DMSO at 10 mM and aliquoted into deep-well plates. The library is an essential tool for predictive toxicology screening and assay development.
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Dispase II solution in PBS, Provitro
Supplier: PROVITRO
Immediately after delivery, place the passage kit in the dark at −20 °C. Prior use thaw the tube. After usage store them in the dark at 2 °C to 8 °C for a maximum of 2 weeks. Take care: After thawing shelf time of Dispase II solution is limited to 14 days.