"protein purification system"
Multiple Affinity Removal Starter Reagent Kit, Agilent Technologies
Supplier: Agilent Technologies
Starter Reagent Kit for Multiple Affinity Removal LC Columns.
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Pierce™ Conjugate Purification Kit, Thermo Scientific
Supplier: Thermo Scientific
The Thermo Scientific Pierce Conjugate Purification Kit is a gentle affinity chromatography system for purifying horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated antibodies from unreacted enzyme after labeling.
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HaloTag Complete Pull-Down System, 1 each, Promega
Supplier: Promega Corporation
The HaloTag Mammalian Pull-Down Systems capture and purify intracellular protein complexes for correlative cellular localization and real-time imaging studies with the same genetic construct for further understanding of overall protein function.
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Pierce™ Protein IgG Plus Orientation Kit, Thermo Scientific
Supplier: Thermo Scientific
Pierce™ Protein IgG Plus Orientation Kits uses a crosslinker to covalently attach antibody that is bound to Protein A (or Protein G) agarose resin, thereby creating a reusable affinity column for purification of antigens.
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Pierce™ Protein G Agarose, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Protein G and Protein G Plus Agarose is a premium-quality affinity resin for antibody purification.
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PureCube Glutathione MagBeads
Supplier: Cube Biotech
The protein purification with the PureCube Glutathione MagBeads and the GST-tag exploit the specific enzyme-substrate binding to gain high levels of purity while maintaining a high protein yield.
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Profinity eXact™ Purification and Tag Cleavage Consumables, Bio-Rad
Supplier: Bio-Rad
Profinity eXact Purification Resin:
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Serum Antibody Purification Kit (Protein A), Abcam
Supplier: Abcam
Serum Antibody Purification Kit (Protein A) (ab109209) is prepared by coupling highly purified protein A to agarose beads and can therefore be used to purify IgG fractions from both serum and ascites fluid.
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PureCube Zr-NTA MagBeads
Supplier: Cube Biotech
PureCube Zr-NTA MagBeads/magnetic beads were developed for the enrichment of phosphorylated proteins. They can also be used for the purification of His-tagged proteins as a secondary function.
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Staphylococcus aureus Recombinant Protein A (from E. coli), HiLyte Fluor® 647
Supplier: Anaspec
Protein A-HiLyte™ Fluor 647 Conjugate can be used as a universal reagent to detect primary antibodies in IHC from many species including rabbit, human, some mouse IgG isotypes, and others. Fluorescence (near-infrared) Excitation/Emission wavelength: 649 nm/674 nm.
AnaSpec's recombinant Protein A consists of only IgG binding domains and was expressed using a recombinant bacterial expression system. Its apparent molecular weight is approximately 30,000 Da compared to 42,000 Da for the native Protein A that is found in Staphylococcus aureus.
Protein A conjugates are widely used as labeled reagents for a variety of experiments including immunoprecipitation, antibody detection and purification and assay development
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Accessories for BAKERBOND® Protein Precipitation and Supported Liquid Extraction Plates
Supplier: Avantor
Vacuum manifold for multi-well plates, J.T.Baker®
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Glutathione Resin for GST Purification, G-Biosciences
Supplier: G-Biosciences
Glutathione Resin is designed for the affinity purification of proteins with a glutathione S-transferase (GST) tag. The resin consists of reduced glutathione (GSH) covalently coupled to 6% cross-linked agarose, via a 10-carbon spacer arm. The resin has a binding capacity of >40mg GST/ml resin.
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Anti-Strep-Tag Rabbit Polyclonal Antibody
Supplier: Bioss
The Strep-tag system is a method which allows the purification and detection of proteins by affinity chromatography. The Strep-tag is a synthetic peptide consisting of eight amino acids (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin, a specifically engineered streptavidin and can be N- or C- terminally fused to recombinant proteins. By exploiting the highly specific interaction, Strep-tagged proteins can be isolated in one step from crude cell lysates. Because the Strep-tag elutes under gentle, physiological conditions it is especially suited for generation of functional proteins.
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NEBExpress Cell-Free E. coli Protein Synthesis System, New England Biolabs
Supplier: New England Biolabs (NEB)
The NEBExpress Cell-free E. coli Protein Synthesis System is an extract-based transcription/translation system derived from E. coli cells that have been engineered for high in vitro synthesis performance. It is designed to synthesize proteins ranging in size from 17 to 230 kDa in just 2 to 4 hours under the control of T7 RNA Polymerase.
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Staphylococcus aureus Recombinant Protein A (from E. coli), HiLyte Fluor® 555
Supplier: Anaspec
Protein A-HiLyte™ Fluor 555 Conjugate can be used as a universal reagent to detect primary antibodies in IHC from many species including rabbit, human, some mouse IgG isotypes, and others. Fluorescence (orange) Excitation/Emission wavelength: 553 nm/568 nm.
AnaSpec's recombinant Protein A consists of only IgG binding domains and was expressed using a recombinant bacterial expression system. Its apparent molecular weight is approximately 30,000 Da compared to 42,000 Da for the native Protein A that is found in Staphylococcus aureus.
Protein A conjugates are widely used as labeled reagents for a variety of experiments including immunoprecipitation, antibody detection and purification and assay development
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Pierce™ Protein A IgG Purification Kit, Thermo Scientific
Supplier: Invitrogen
The Protein A IgG Purification Kit is useful for routine purification of antibodies.
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