129577 Results for: "cell+culture+serum&pageNo=10&view=easy"

Supplier: Immunoreagents

TRITC (Tetramethylrhodamine isothiocyanate) is a popular rhodamine derivative pink in color but emits a red-orange color upon Emission at 580nm. TRITC is frequently paired with FITC in double labeling experiments and conjugated antibodies are often used in immunofluorescence microscopy, flow cytometry, FLISA (fluorescent ELISA) and high throughput screening assays. These conjugates are best excited with the 532 nm spectral line of the He-Ne laser and are also recommended as a second color detection reagent in in situ hybridization applications. (Excitation/Emission = 555nm / 580nm Emission Color = Orange to Red (Similar Dyes: Alexa Fluor 555, Cy3, DyLight 550)

Supplier: Genetex

The INK4a-ARF locus is comprised of two tumor suppressors, p16INK4a and p14ARF. These two proteins are encoded through differential splicing of alternative first exons. The p16INK4a (exon 1alpha) protein inhibits the cyclin D-dependent kinases (CDK) that control the phosphorylation of the Rb protein and cell proliferation. The p14ARF gene product complexes with the MDM2 protein within the nucleus, thus modulating the activity of the p53 protein. P14ARF is a potent tumor suppressor in the presence of wild-type p53, while mutant p53 substantially reduces growth inhibition by p14ARF.

Supplier: Prosci

Trail Antibody: Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel member in the TNF family was recently identified and designated TRAIL (for TNF-related apoptosis-inducing ligand) and Apo-2L (for Apo-2 ligand)1, TRAIL is a type II membrane protein and expressed in a variety of human tissues. Two novel death domain containing receptors DR4 and DR5 have been identified as the receptor for TRAIL3-6. Like TNF and Fas ligand, TRAIL induces apoptosis and NF-kappa B activation in many tissues and cells.

Supplier: Genetex

This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. The N-terminus of this gene contains a mitochondrial targetting signal, essential for mitochondrial localization.

Supplier: Proteintech

14-3-3 gamma (also known as YWHAG) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody specifically recognizes gamma isoform of 14-3-3.

Supplier: Prosci

PTP mu, a R2A receptor protein tyrosine phosphatase, is composed of an extracellular segment containing a MAM domain, an immunoglobulin domain and four fibronectin type III repeats, a transmembrane segment, and two intracellular protein phosphatase domains. PTP mu has been shown to affect cell-cell aggregation, regulate cardiac myocyte Kv1.5 channel expression, and promote neurite outgrowth of retinal ganglion cells. The human PTP mu gene is localized on chromosome 18pter-q11, a region with frequent abnormalities implicated in human cancer. PTP mu may be important in responses to pathological conditions associated with the loss of cell-cell interactions in the heart.

Supplier: Prosci

TAO (thousand and one amino acid) protein kinases mediate signaling from carbachol to p38 MAPK and ternary complex transcription factors. This has led to the proposal that the TAO protein kinases play an essential role in signaling from physiological agonists to the stress-responsive p38 MAPKs. Autophosphorylation of TAO may play a role in the mechanism of TAO activation. The MEK binding domain of TAO is autophosphorylated on both serine and threonine residues and Ser181 is located within this domain.

Supplier: VWR International

3-D rotator waver is ideal for blood samples, DNA extractions, blotting techniques, and general mixing of various size tubes.

Supplier: VWR International

USB Datalogging ULT freezer thermometer that can be used in 21 CFR 11 environments raw data is locked and may not be cleared/changed on base unit.

Supplier: VWR International

3-D rotator waver is ideal for blood samples, DNA extractions, blotting techniques, and general mixing of various size tubes.

Supplier: VWR International

VWR® Series –80 °C ultra-low temperature (ULT) freezers, powered by the H-Drive refrigeration system, are designed for efficiency. The units enable the same excellent experience as upright ULT freezers, with an additional benefit of enhanced performance. As a result, the series delivers a foundational ULT experience with the reliability and performance needed to protect your work—and the sustainability to meet global compliance.

Supplier: VWR International

This microscope is a live cell microscope platform for advanced microbiology. The instrument can be upgraded with Fluorescence for research tasks, while the 'Light memory' function and the Auto ON/OFF mode enables a fast and safe throughput of samples.

Supplier: Globe Scientific

Globe Glass™ media bottles are designed with a wide mouth for ease of filling, dispensing, and pipetting. A drip-free pour ring and autoclavable polyethylene (PE) screw cap enable the sterilization of media and reagents. Globe Glass™ media bottles are available in sizes ranging from 50 to 10000 ml.

Supplier: Prosci

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer (reviewed in 1). Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNF-R1 and Fas. Several death receptors including DR3, DR4, DR5, and DR6 have been identified. Like TNF-R1, DR6 (also known as TNFRSF21) interacts with death domain containing adapter molecule TRADD. Overexpression of DR6 induces apoptosis and activates NF-kB and JNK. DR6 is widely expressed in human tissues and cell lines (2). Recently, DR6 was found to interact with an amino-terminal fragment of the Beta-amyloid protein (APP) in neurons, activating a caspase 6-dependent apoptotic event leading to axonal degeneration and pruning (3). The doublet observed in the SDS-PAGE is likely due to incomplete removal of the signal sequence.

Supplier: Prosci

Mucin-1 is a 265-400kDa glycoprotein. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit and the C-terminal beta subunit. . The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. MUC1 may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions.

Supplier: Quantabio

These easy-to-use and highly efficient kits are designed for the synthesis of first-strand cDNA using your choice of cDNA priming method

Supplier: Prosci

This antibody recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to ErbB2, -3 -4 or HER2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head/neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ TDE Series –80 °C ultra-low temperature (ULT) freezers, powered by the H-Drive system, are designed for efficiency. As a result, the series delivers a foundational ULT experience with the reliability and performance needed to protect your work—and the sustainability to meet global compliance.

Supplier: Transforming Technologies

The ESD Cleanroom Frock is engineered to meet Class 100 cleanroom demands for industries such as microelectronics, semi-conductors, disk drives, laser, and similar industries. Cleanroom Frocks are made with static dissipative fabric to eliminate static on the wearer. Superior workmanship and reliable panel-to-panel continuity makes the 6200 ESD Cleanroom Frock an excellent product to control contamination and static.

Supplier: Thermo Scientific

Automate solvent-free solid phase micro extraction (SPME) sample preparation by performing sample extraction and enrichment in a single step using Thermo Scientific™ SPME Arrow and SPME Fiber products. These products are used with the Thermo Scientific™ TriPlus RSH™ autosampler prior to gas chromatography (GC) or gas chromatography mass spectrometry (GC/MS) analysis.

Supplier: MP Biomedicals

SDS is an anionic detergent typically used to solubilize and denature proteins for electrophoresis.
SDS is used to solubilize and denature protein for Protein Electrophoresis & Blotting. SDS has also been used in large-scale phenol extraction of RNA to promote the dissociation of protein from nucleic acids when extracting from biological material. Surfactant used for mild, efficient, and greener dethioacetalization.
Store at Room Temperature (15-30 °C).

Supplier: ReVacc Scientific

This kit is developed for serologic test for human IgA titer of Anti-SARS-CoV-2 trimeric Spike (Wuhan Strain) antibody in serum/plasma or purified human antibody (monoclonal and polyclonal) in vitro. Antibodies of IgM and IgA class are a sign of an active infection (primary infection and reactivation) and disappear during convalescence. In some cases they can persist for several months.

Supplier: Prosci

PD-1 Antibody: Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases.

Supplier: Prosci

Thyroid-stimulating hormone (TSH, also known as thyrotropin) is a glycoprotein involved in the control of thyroid structure and metabolism, which stimulates the release of the thyroid hormones. TSH is regulated by thyroid hormone (T3) and various retinoid compounds. TSH binds to the thyroid-stimulating hormone receptor (TSHR), which is cleaved into two subunits, A and B, and plays a major role in regulating thyroid function. The third cytoplasmic loop of TSHR has been identified as critical for its role in regulating inositol phosphate and cAMP formation. In Graves disease, an autoimmune disorder, TSHR is activated by autoantibodies, which may be stimulated by the cleavage of the A and B subunits.

Supplier: Prosci

CARMA3 Antibody: CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules. CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types. As with CARMA1, the CARD domain of CARMA3 has been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappa B activation. When expressed in cells, this protein binds to BCL10 and activates NF-kappa B Recent experiments have shown that CARMA3 is required for EGF-induced NF-kappa B activation and contributes to tumor growth in vivo, suggesting that CARMA3 may serve as a new therapeutic target for the treatment of EGFR-driven tumors.

Supplier: Prosci

This mAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

Supplier: Prosci

PANX1 belongs to the innexin family. Innexin family members are the structural components of gap junctions. This protein and pannexin 2 are abundantly expressed in central nerve system (CNS) and are coexpressed in various neuronal populations. Studies in Xenopus oocytes suggest that this protein alone and in combination with pannexin 2 may form cell type-specific gap junctions with distinct properties.The protein encoded by this gene belongs to the innexin family. Innexin family members are the structural components of gap junctions. This protein and pannexin 2 are abundantly expressed in central nerve system (CNS) and are coexpressed in various neuronal populations. Studies in Xenopus oocytes suggest that this protein alone and in combination with pannexin 2 may form cell type-specific gap junctions with distinct properties.

Supplier: Electron Microscopy Sciences

For TEM and Metallography - Model MC2
The MicroPol™ Polisher is a compact, versatile, electrically controlled, precise mechanical specimen grinder/polisher designed for planar grinding, thinning and polishing of specimen in the fields of nanotechnology, semiconductors, and materials science. The parameters of polishing (speed, load, time, etc.) can be matched according to the properties of the specimen. High quality samples can be prepared with minimal effort by the operator.

Supplier: Electron Microscopy Sciences

Nominal calibrated dimensions: 300 or 700 nm.

Supplier: STEMCELL Technologies

Interleukin 2 (IL-2) is a monomeric cytokine that was originally identified as a T cell growth factor (Gaffen and Liu). It binds to heterotrimeric receptors consisting of CD25, CD122, and CD132. Upon binding, it activates JAK3-, STAT5-, and AKT-dependent signaling pathways, which results in cellular proliferation and survival (Ma et al.). The majority of IL-2 is secreted by activated CD4+ and CD8+ T cells, although B cells and dendritic cells were found to produce IL-2 in small amounts. IL-2 downregulates immune responses to prevent autoimmunity during thymic development, influences the development of CD4+CD25+ regulatory T cells, and affects development of follicular helper T cells. IL-2 also controls inflammation by inhibiting Th17 differentiation (Banchereau et al.). High IL-2 levels in serum are associated with progression of scleroderma, rheumatoid arthritis, and gastric and non-small cell lung cancer, though no known disease can be directly attributed to the lack or excess of IL-2 (Gaffen and Liu).