buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
2.5 M Ammonium citrate/ Ammonium hydroxide buffer pH 8.5 , Rigaku
Supplier: Rigaku Reagents
2.5 M Ammonium citrate/ Ammonium hydroxide buffer pH 8.5 , Rigaku
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EndoTrap® HD 1/1, Biovendor
Supplier: BioVendor
EndoTrap® HD removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate.
The EndoTrap® HD resin is especially designed to meet the needs of pharma and biotech industry for the efficient removal of endotoxins in biomanufacturing processes as well as any application that requires highest endotoxin removal rates.
EndoTrap® HD fulfils all requirements for industrial applications, and it can easily be integrated in/at early or late-stage purification processes. Moreover, the High-Definition Matrix is also available for small-scale purification as pre-filled columns.
Endotoxin removal with EndoTrap® HD works effectively in the pH range of 4-9 and NaCl concentrations of 50-600 mM. Buffers such as HEPES, TRIS, MOPS, MES, and PIPES are recommended. Citrate buffers and chelators of divalent cations (like EDTA) have to be avoided
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Pierce Triethylammonium Bicarbonate, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Triethylamonium bicarbonate (TEAB) is a 10X dissolution buffer for isobaric mass tag labeling experiments.
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PBS Endotoxin-free (sterile), AdipoGen Life Sciences
Supplier: Adipogen
Endotoxin free buffer used for the dilution of TLRpure LPS and solubilisation of endotoxin-free reagents (e.g
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pH Singles Calibration Pouches, Oakton Instruments
Supplier: OAKTON INSTRUMENTS MS
Calibrate your pH meter in the lab or in the field with convenient, portable, and highly accurate pH Singles
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Endotoxin Removal System, EndoTrap® HD, Biovendor
Supplier: BioVendor
EndoTrap® HD removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples.
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Pierce™ Blocker™ Casein in PBS, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Blocker Casein in PBS or TBS is a ready-to-use, solution of purified casein protein for blocking steps in Western blot, ELISA, IHC and nucleic acid detection methods.
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Rapid GST Inclusion Body Solubilization and Renaturation Kit, Cell Biolabs
Supplier: Cell Biolabs
Recombinant proteins expressed in bacteria often form inclusion bodies, especially when they are expressed at high levels
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StabilCoat® BSA ELISA Stabilizer/Blocker, Surmodics
Supplier: Surmodics
StabilCoat® Immunoassay Stabilizer, and the 5X concentrate, effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. The product can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper.
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StabilGuard® Immunoassay Stabilizer, Surmodics IVD, Inc.
Supplier: Surmodics
StabilGuard® Immunoassay Stabilizer (BSA-Free) effectively preserves the conformation and activity of dried proteins while simultaneously blocking the surface to reduce non-specific binding. It can be used to stabilize antibodies, antigens or enzymes on an assortment of immunoassay components including polystyrene plates, tubes, glass, membranes, and filter paper.
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StabilBlot™ Casein Blocker-5X, TBS, BioFX®, Surmodics IVD, Inc.
Supplier: Surmodics
The StabilBlot™ Blocker Family offers a range of options, both protein-based and synthetic-based, to block non-specific binding as well as enhance sensitivity when used as a blocker and antibody diluent.
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Pierce Y-PER™, Yeast Protein Extraction Reagent, Thermo Scientific
Supplier: Invitrogen
Y-PER™ reagent uses a mild detergent lysis procedure to efficiently release functionally active solubilized proteins.
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Hank's Balanced Salt Solution (HBSS), Modified (Without Ca⁺⁺ and Mg⁺⁺)
Supplier: Stemcell Technologies
Hank's Balanced Salt Solution (HBSS) can be used for diluting, washing, irrigating or as a transporting solution for cell or tissue culture. It provides a buffering system to maintain the physiological pH range and osmotic balance of culture media, and provides cells with a source of water and essential inorganic ions and a carbohydrate as an energy source.
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Pierce Subcellular Protein Fractionation Kit, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Subcellular Protein Fractionation Kit for Cultured Cells enables segregation and enrichment of proteins from five different cellular compartments.
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PIPES/ Sodium hydroxide pH 6.0, 1M, Rigaku
Supplier: Rigaku Reagents
PIPES/ Sodium hydroxide pH 6.0, 1M, Rigaku
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AdvanWash™ washing solution, Advansta
Supplier: ADVANSTA INC MS
Optimized washing solutions, for Western blotting with WesternBright systems.
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