buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Western Blot Stripping Buffers
Supplier: Li-Cor
Stripping and reprobing a western blot is a common laboratory method.
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Wizard SV 96 Cell Lysis Solution, Promega®
Supplier: Promega Corporation
Cell Lysis Solution is a component of the Wizard MagneSil, Wizard Plus and Wizard SV 96 and SV 9600 Plasmid DNA Purification Systems and the PureYield Plasmid Midiprep System.
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RNAgents® Denaturing Solution, Promega
Supplier: Promega Corporation
RNAgents Denaturing Solution lyses cells or tissue under conditions that rapidly inhibit ribonucleases.
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Luna Cell Ready Lysis Module, New England Biolabs
Supplier: New England Biolabs (NEB)
Cell cultures are often analyzed for gene expression or treatment responses as a proxy for a living organism. Traditionally, RNA is extracted and purified from treated cells via column-based or chemical methods. The Luna Cell Ready Lysis Module is a fast, convenient, and sensitive alternative for evaluating RNA expression levels without performing traditional RNA extraction steps.
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G-Biosciences Antigen Retrieval Solution, Citrate (pH 6.0) (50X)
Supplier: G-Biosciences
Solution developed to unmask antigens in paraffin embedded tissue sections.
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ZymoBIOMICS Lysis Solution, Zymo Research
Supplier: Zymo Research
ZymoBIOMICS Lysis Solution is designed to be used with the ZymoBIOMICS DNA extraction kits. Used in combination with the ZR BashingBead Lysis Tubes, it assists with microbial lysis. It is certified to be low-bioburden to ensure minimal background contaminants for low abundance microbiome samples.
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FISH-Blocker™ Non-Animal Sera Protein Blocking Agent, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' FISH-Blocker™ is uses a fish protein as the primary blocking agent to eliminate cross reactivity
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Potassium Acetate 5.0 M, Rockland Immunochemicals
Supplier: Rockland Immunochemical
5.0 M Potassium Acetate (untitrated) DEPC Treated
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GenScript® Broad Multi Color Pre-Stained Protein Standard, GenScript
Supplier: Genscript
GenScript offers a wide selection of Protein molecular weight standards for SDS-PAGE and Western blot, including prestained, unstained, peptide, Western-viewable, and other standards.
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5X Detector™ Block, KPL Inc.
Supplier: KPL
Detector Block is recommended as a general blocking agent for use in membrane-based detection assays
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GenScript® PAGE-MASTER Protein Standard (for SDS-PAGE), GenScript
Supplier: Genscript
PAGE-MASTER Protein Standard, Consists of seven bands with molecular weight of 10 kD, 20 kD, 30 kD, 40 kD, 60 kD, 80 kD and 120 kD, All bands are highly purified, Volume: Load 5 ul/well for a mini-gel, Application: For SDS-PAGE gels, Storage: -20deg C, Size: 500ul
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NAP-BLOCKER™ Non-Animal Protein Blocking Agent, G-Biosciences
Supplier: G-Biosciences
A major drawback of current blocking solutions, such as BSA, casein and milk powder, is that they are derived from animal sources
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Pierce™ SuperBlock™ Dry Blend Blocking Buffer, Thermo Scientific
Supplier: Invitrogen
SuperBlock (TBS) Blocking Buffer is an optimised TBS solution containing a protein that provides incredibly fast and effective blocking for ELISA, IHC and Western Blot Analysis.
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Pierce™ LDS Sample Buffer, Non-Reducing (4X), Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce LDS Sample Loading Buffer (4X) is a nonreducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes.
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Phosphate Buffered Saline, 10X, Advanced Biomatrix
Supplier: Advanced Biomatrix
Use With Type I Collagen to help form 3D hydrogels.
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Monarch® RNase A, New England Biolabs
Supplier: New England Biolabs (NEB)
Monarch® RNase A is a component of the Monarch® Genomic DNA Purification Kit (NEB #T3010), which can be used to purify genomic DNA from a variety of sample types.
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