buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Running Buffers and Reagents, Protein Electrophoresis, Bio-Rad
Supplier: Bio-Rad
Bio-Rad offers a range of running buffers and reagents for many types of gels including SDS PAGE, nondenaturing, isoelectric focusing (IEF), MOPS and MES for Bis-Tris gels, and Tris-Tricine
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Endo Trap® HD Buffer Kit, BioVendor
Supplier: BioVendor
EndoTrap® HD removes endotoxins (lipopolysaccharides/LPS) from aqueous solutions of a wide range of samples such as proteins, antibodies, vaccines, nucleic acids, buffers and cell extracts with excellent sample recovery rate.
The EndoTrap® HD resin is especially designed to meet the needs of pharma and biotech industry for the efficient removal of endotoxins in biomanufacturing processes as well as any application that requires highest endotoxin removal rates.
EndoTrap® HD fulfils all requirements for industrial applications, and it can easily be integrated in/at early or late-stage purification processes. Moreover, the High-Definition Matrix is also available for small-scale purification as pre-filled columns.
Endotoxin removal with EndoTrap® HD works effectively in the pH range of 4-9 and NaCl concentrations of 50-600 mM. Buffers such as HEPES, TRIS, MOPS, MES, and PIPES are recommended. Citrate buffers and chelators of divalent cations (like EDTA) have to be avoided
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10X PBS with 0.5% Tween 20, Teknova
Supplier: Teknova
Tween 20 is a frequently used member of the polysorbate family of detergents.
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Pierce™ Electrophoresis Running Buffers, Thermo Scientific
Supplier: Invitrogen
These are space-saving stock solution that is ideal for quickly preparing running buffer used for gel electrophoresis.
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EndoToxFree™ PBS (1X), pH 7.4, USP Grade
Supplier: QUALITY BIOLOGICAL, INC.
EndoToxFree™ PBS (1X), USP Grade is a ready-to-use phosphate-buffered saline solution with endtoxin levels <0.005 EU/ml. This PBS formulation is manufactured with USP-grade ingredients. It is not a USP reference standard solution.
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Pierce™ Wash Buffers, Thermo Scientific
Supplier: Invitrogen
These wash buffers are designed for use in dialysis, cross-linking, enzyme assays, ELISAs, immunohistochemistry, protein plate-coating, biotinylation applications.
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Background Blocker, Diagnostic Biosystems
Supplier: Diagnostic Biosystems
To reduce nonspecific staining during IHC.
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Pierce™ 10X TBE Buffer, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce 10X TBE Buffer is a space-saving stock solution that is ideal for casting and preparing Tris-Borate-EDTA (pH 8.3) running buffer used for agarose and polyacrylamide gel electrophoresis of nucleic acids.
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Pierce™ RIPA Buffer, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific RIPA Buffer is a high-quality, ready-to-use and fully disclosed formulation of a popular cell lysis reagent for cultured mammalian cells.
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Poly HRP Dilution Buffer, Thermo Scientific
Supplier: Invitrogen
Thermo Scientific Pierce Poly-HRP Dilution Buffer is optimized to ensure optimal performance with Poly-HRP Streptavidin.
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Monarch® Precipitation Enhancer, New England Biolabs
Supplier: New England Biolabs (NEB)
The Monarch® precipitation enhancer solution, supplied as a component of the Monarch® HMW DNA extraction kit for cells and blood, is a salt-based solution that facilitates the precipitation of DNA onto the Monarch® DNA capture beads during HMW DNA extraction from cells and blood.
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Sample Buffers and Reagents, Protein Electrophoresis, Bio-Rad
Supplier: Bio-Rad
Sample buffers are available for numerous applications, including native (nondenaturing) PAGE, SDS-PAGE, zymograms, and IEF
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Pierce BupH™ Dry Blend Buffers, Washing, Thermo Scientific
Supplier: Invitrogen
BupH™ packs are pre-blended and pre-measured dry mixtures of buffers that are easy to prepare and used in protein purification and common laboratory techniques.
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10x EDTA buffer pH 8.0, Novus Biologicals
Supplier: Novus Biologicals
10x EDTA buffer pH 8.0.
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BLOT-QuickBlocker™ Modified Milk Protein Blocking Agent, G-Biosciences
Supplier: G-Biosciences
G-Biosciences' BLOT-QuickBlocker™ is a novel modified milk protein that is highly soluble and does not inhibit peroxidase detection
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Cell Lysis Solution (Genomic Purification), 1 liter, Promega
Supplier: Promega Corporation
An easy, solution-based method for isolation of DNA from white blood cells, tissue culture cells, animal tissue, plant tissue, yeast and Gram-positive and Gram-negative bacteria.
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